Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,234,629	A→G	I30T (ATC→ACC)	nhaB ←	sodium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,234,629	0	A	G	100.0%	101.2 / NA	28	I30T (ATC→ACC)	nhaB	sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

ATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCA  >  NC_000913/1234482‑1234770                                                                                                                                                    |                                                                                                                                              aTGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGtt                                                                                                                                             >  2:225858/1‑150 (MQ=255)                 cTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGc                                                                                                                            >  2:590269/1‑151 (MQ=255)                                 accaccGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTct                                                                                                            <  1:625846/151‑2 (MQ=255)                                              agcGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGccc                                                                                                <  1:290516/150‑1 (MQ=255)                                              agcGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGccc                                                                                                >  2:433329/1‑150 (MQ=255)                                                        aTTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTgcg                                                                                     >  2:166375/1‑151 (MQ=255)                                                             aGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCAt                                                                                 >  2:482157/1‑150 (MQ=255)                                                             aGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATa                                                                                <  2:219243/151‑1 (MQ=255)                                                             aGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGATTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCAt                                                                                 >  2:447150/1‑150 (MQ=255)                                                               ggCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAgc                                                                              <  1:225858/151‑1 (MQ=255)                                                                 ccATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAgcgc                                                                            <  1:507670/151‑1 (MQ=255)                                                                                aaataaatTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTCCCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGAt                                                             <  2:316886/151‑1 (MQ=255)                                                                                    aaatTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTcc                                                         >  2:105861/1‑151 (MQ=255)                                                                                     aatTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTcc                                                         >  1:669305/1‑150 (MQ=255)                                                                                             tACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGtt                                                >  1:114324/1‑151 (MQ=255)                                                                                                       cAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCccttacct                                      <  2:135693/151‑1 (MQ=255)                                                                                                       cAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCccttacct                                      <  1:547692/151‑1 (MQ=255)                                                                                                         gCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCccttacctta                                    <  2:629060/151‑1 (MQ=255)                                                                                                              cgacgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaat                               >  2:125651/1‑151 (MQ=255)                                                                                                                 cgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaataa                             <  2:554362/150‑1 (MQ=255)                                                                                                                              gaGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGttttt               <  1:101053/151‑1 (MQ=255)                                                                                                                              gaGGAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGtttt                <  1:645276/150‑1 (MQ=255)                                                                                                                                 gAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATg            >  2:19562/1‑151 (MQ=255)                                                                                                                                 gAAAATTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATg            <  2:369976/151‑1 (MQ=255)                                                                                                                                  aaaaTTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGt           >  2:626887/1‑151 (MQ=255)                                                                                                                                     aTTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGttt         >  1:154668/1‑150 (MQ=255)                                                                                                                                     aTTAACGGGTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTGTATGTTTa        <  1:363755/151‑1 (MQ=255)                                                                                                                                           gggTTTACGGTTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCa  <  1:613060/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              ATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAGTGAAAATAAATTCCGCTACCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATGTTTAAACGCA  >  NC_000913/1234482‑1234770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑