Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,241,779	A→G	L98P (CTG→CCG)	cvrA ←	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,241,779	0	A	G	100.0%	145.0 / NA	41	L98P (CTG→CCG)	cvrA	putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/15);  total (26/15)

TTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACG  >  NC_000913/1241641‑1241925                                                                                                                                           |                                                                                                                                                   ttACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCg                                                                                                                                        >  1:518224/1‑151 (MQ=255)  tACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGc                                                                                                                                         <  1:434442/149‑1 (MQ=255)   accaccCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGAc                                                                                                                                      >  2:134058/1‑151 (MQ=255)                       ccGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                                                                                  >  2:429542/1‑151 (MQ=255)                       ccGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCcac                                                                                                                  >  2:135560/1‑151 (MQ=255)                          cTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACg                                                                                                               >  2:330866/1‑151 (MQ=255)                           tGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACg                                                                                                               >  2:320883/1‑150 (MQ=255)                                         agCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTg                                                                                                >  2:65085/1‑151 (MQ=255)                                             gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTg                                                                                            >  1:555548/1‑151 (MQ=255)                                             gacgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTg                                                                                            >  2:165677/1‑151 (MQ=255)                                               cgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGt                                                                                             >  1:454174/1‑148 (MQ=255)                                               cgaTAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTgcg                                                                                          >  1:527864/1‑151 (MQ=255)                                                   aGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCAt                                                                                       <  2:143007/150‑1 (MQ=255)                                                        cGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCccgcc                                                                                 >  1:102666/1‑151 (MQ=255)                                                            taataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTc                                                                              <  2:527100/150‑1 (MQ=255)                                                            taataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCg                                                                             >  2:489643/1‑151 (MQ=255)                                                             aataaGCCTTCAACCAAATCAAAATTAAACATCCACGCCGCCATCATGCCGGTTAAGCCAGATGTGATAAGCTCGCCCGGCGGCGCCGTCGACAGTCCCTTTCCTAACGCCACACGAAAGGAATTGGCTGGAGTGCGCATCTCGCCGTCgt                                                                            <  1:204574/151‑2 (MQ=255)                                                              ataaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAg                                                                           <  1:479004/151‑1 (MQ=255)                                                                aaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGaa                                                                                                        <  2:22456/120‑1 (MQ=255)                                                                aaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGaa                                                                                                        >  1:22457/1‑120 (MQ=255)                                                                aaGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAg                                                                           >  1:15960/1‑149 (MQ=255)                                                                      tcaatcaaATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAaata                                                                    >  2:562470/1‑150 (MQ=255)                                                                        aatcaaatcaAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATACCGCCGTCGAGCAAAATAATc                                                                 >  1:492870/1‑151 (MQ=255)                                                                                   gATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAg                                                      <  2:638481/151‑1 (MQ=255)                                                                                    aTTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAg                                                      <  1:429537/150‑1 (MQ=255)                                                                                       aaaCAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGtt                                                   >  2:233791/1‑150 (MQ=255)                                                                                          cAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttactt                                               >  1:441196/1‑151 (MQ=255)                                                                                            gCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGttacttac                                             <  1:261877/151‑1 (MQ=255)                                                                                               aCGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCAt                                          <  2:322130/151‑1 (MQ=255)                                                                                                 gccgccATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGt                                        >  2:164904/1‑151 (MQ=255)                                                                                                      catcatGCCGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCg                                   >  2:251998/1‑151 (MQ=255)                                                                                                             ccGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAgggg                             >  1:234608/1‑150 (MQ=255)                                                                                                             ccGGTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGt                            <  1:134054/151‑1 (MQ=255)                                                                                                                gTTAAACCAGAGGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAAt                         <  2:102676/151‑1 (MQ=255)                                                                                                                          agGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACgg               <  2:102669/151‑1 (MQ=255)                                                                                                                          agGTGATAAGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACgg               <  1:419351/151‑1 (MQ=255)                                                                                                                                 aaGCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAGCGGGATAccg        >  2:86079/1‑151 (MQ=255)                                                                                                                                   gCACGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATAccgcc      <  1:575558/151‑1 (MQ=255)                                                                                                                                     aCGCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATAccgccg     >  2:401638/1‑150 (MQ=255)                                                                                                                                       gCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGAc   >  2:651172/1‑150 (MQ=255)                                                                                                                                       gCCCGGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACg  >  2:160984/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                   TTACCACCCAGCAGAGAAAAGACCGCTGCAGCATCGGTAGAGCCGACGATAGCGCCGATTAATAAGCCTTCAATCAAATCAAGATTAAACAGCCACGCCGCCATCATGCCGGTTAAACCAGAGGTGATAAGCACGCCCAGCGTCGCCAGCGACAGTGCCGGTCCTAACGCCACACGAAAGGAACTGGCCTGAGTGCGCATCCCGCCGTCGAGCAAAATAATCGCCAGTGCCAGGTTACTTACCATGTAGGCGAAGGGGTAATTATCAAACGGGATACCGCCGACG  >  NC_000913/1241641‑1241925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑