Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,045	T→C	Q288Q (CAA→CAG)	puuA ←	glutamate‑‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,045	0	T	C	100.0%	146.9 / NA	38	Q288Q (CAA→CAG)	puuA	glutamate‑‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/17);  total (21/17)

CGATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACG  >  NC_000913/1359911‑1360189                                                                                                                                       |                                                                                                                                                 cgATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTgatatgga                                                                                                                                  <  1:227483/151‑1 (MQ=255)     aCGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTgatatggatatg                                                                                                                              >  2:159216/1‑151 (MQ=255)           tCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTc                                                                                                                         <  1:26096/150‑1 (MQ=255)               gTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTg                                                                                                                    <  2:633221/151‑1 (MQ=255)                       ccAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTg                                                                                                            >  1:326287/1‑151 (MQ=255)                             aCGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTc                                                                                                      >  1:117395/1‑151 (MQ=255)                              cGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTc                                                                                                      >  1:652836/1‑150 (MQ=255)                                ccATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATa                                                                                                   <  2:496968/151‑1 (MQ=255)                                ccATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATa                                                                                                   <  1:654600/151‑1 (MQ=255)                                          cgGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGcc                                                                                         <  2:326289/151‑1 (MQ=255)                                                   gTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTg                                                                                >  2:255779/1‑151 (MQ=255)                                                          atcCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGc                                                                          >  1:16848/1‑150 (MQ=255)                                                               ggCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTa                                                                    <  1:44276/151‑1 (MQ=255)                                                               ggCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTa                                                                    <  1:569811/151‑1 (MQ=255)                                                                 cGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATg                                                                  >  2:269184/1‑151 (MQ=255)                                                                    gCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCtt                                                               <  1:201907/151‑1 (MQ=255)                                                                      aTCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCtttt                                                             >  2:196274/1‑151 (MQ=255)                                                                               agcagcGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATcg                                                    >  1:448790/1‑150 (MQ=255)                                                                                      gcgAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCAc                                             <  2:203294/151‑1 (MQ=255)                                                                                        gAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCAcc                                            >  2:393648/1‑150 (MQ=255)                                                                                        gAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCa                                           <  1:139327/151‑1 (MQ=255)                                                                                           tCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAgg                                         >  1:168368/1‑150 (MQ=255)                                                                                              tCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAgg                                         <  1:52227/147‑1 (MQ=255)                                                                                              tCGCCTTCCGCGTCAGAAAGCACGTTCTAGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGATCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGtt                                     >  1:446414/1‑151 (MQ=255)                                                                                               cGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGttt                                    <  2:618699/151‑1 (MQ=255)                                                                                                   ttCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGt                                >  1:156282/1‑151 (MQ=255)                                                                                                    tCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTg                               >  1:210455/1‑151 (MQ=255)                                                                                                     ccGCGTCAGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCCTCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGc                              >  2:335610/1‑151 (MQ=255)                                                                                                            aGAAAGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGca                       <  1:337399/151‑1 (MQ=255)                                                                                                              aaaGCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGcatc                     >  2:71792/1‑151 (MQ=255)                                                                                                                 gCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCatcatc                  <  1:535721/151‑1 (MQ=255)                                                                                                                 gCACGTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCatcatc                  >  2:659805/1‑151 (MQ=255)                                                                                                                     gTTCTCGCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGc                >  1:386757/1‑149 (MQ=255)                                                                                                                           gCCACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTcc        <  2:515453/151‑1 (MQ=255)                                                                                                                             cACGATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTcc        >  1:652983/1‑149 (MQ=255)                                                                                                                                gATTATTCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCAc   >  1:174521/1‑151 (MQ=255)                                                                                                                                 attattCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACg  <  2:39048/151‑1 (MQ=255)                                                                                                                                  ttattCTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACg  >  1:231871/1‑150 (MQ=255)                                                                                                                                       |                                                                                                                                                 CGATACGAGTTCACGTTTGGTGCCAGCAACGCCATCGACGACGGCATCAGGTCAATCATCCCGGCGAGCATCTTTTTCAGCAGCGGCGAATCTTCGCCTTCCGCGTCAGAAAGCACGTTCTCGCCACGATTATTTTGCATACTGATATGGATATGCATTCCGCTGCCCGCGTGCTCTTCATACGGCTTCGCCATAAAAGTGGCGTGCATCTTATGCTTTTCTGCCATCAGACGCACCAGGCGTTTTAGTGCCAGCGCATCATCGCAGGCTTCCAGCACG  >  NC_000913/1359911‑1360189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑