Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,363,578	A→G	D279G (GAC→GGC)	puuC →	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,363,578	0	A	G	100.0%	176.9 / NA	52	D279G (GAC→GGC)	puuC	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase; succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

GCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGC  >  NC_000913/1363432‑1363721                                                                                                                                                   |                                                                                                                                                gcTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGc                                                                                                                                             >  1:540781/1‑151 (MQ=255)   tGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGccc                                                                                                                                           >  1:518817/1‑151 (MQ=255)       gcgTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGAt                                                                                                                                       >  2:587945/1‑151 (MQ=255)       gcgTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGAt                                                                                                                                       >  1:266954/1‑151 (MQ=255)        cgTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGAtt                                                                                                                                      >  1:181557/1‑151 (MQ=255)            aTAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCa                                                                                                                                  <  2:136356/151‑1 (MQ=255)                 gATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAgg                                                                                                                             >  1:467580/1‑151 (MQ=255)                       gACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCaa                                                                                                                       <  2:480778/151‑1 (MQ=255)                        aCGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCa                                                                                                                        >  2:5805/1‑149 (MQ=255)                            cATTGCCTTTACCGTTTCAACCCGTACCGGGAAACAGCTGCTGAAAGAGGCGGGCGACAGCAACATGAAACGCGTCTGTCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAgcg                                                                                                                    <  1:658826/149‑1 (MQ=255)                                 ccTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCgc                                                                                                             <  2:117313/151‑1 (MQ=255)                                    ttACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCgca                                                                                                            <  1:581839/149‑1 (MQ=255)                                            tCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCAtt                                                                                                   <  2:228754/150‑1 (MQ=255)                                             cAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCAttt                                                                                                  <  1:564478/150‑1 (MQ=255)                                                  cGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCa                                                                                                                        <  1:444507/123‑1 (MQ=255)                                                  cGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCa                                                                                                                        >  2:444512/1‑123 (MQ=255)                                                   gTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACaa                                                                                           <  1:126197/151‑1 (MQ=255)                                                      ccGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAAcc                                                                                         >  1:489046/1‑150 (MQ=255)                                                        gggAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAgg                                                                                      >  2:203065/1‑151 (MQ=255)                                                            aaCAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGAc                                                                                   >  1:665412/1‑150 (MQ=255)                                                            aaCAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACa                                                                                  <  2:608342/151‑1 (MQ=255)                                                             aCAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAg                                                                                 <  2:247825/151‑1 (MQ=255)                                                              cAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAgg                                                                                >  2:334723/1‑151 (MQ=255)                                                                  tgctgAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGtgtg                                                                            >  2:417857/1‑151 (MQ=255)                                                                    ctgAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGc                                                                           >  2:398419/1‑150 (MQ=255)                                                                           aTGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCgg                                                                   <  2:173013/151‑1 (MQ=255)                                                                               gggCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAAcg                                                               >  1:516553/1‑151 (MQ=255)                                                                                        cAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTg                                                      <  1:485999/151‑1 (MQ=255)                                                                                          aCATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCt                                                    <  1:298955/151‑1 (MQ=255)                                                                                                 aCGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAagag                                             <  2:588423/151‑1 (MQ=255)                                                                                                 aCGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAagag                                             <  2:540786/151‑1 (MQ=255)                                                                                                     gTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCa                                         <  2:294516/151‑1 (MQ=255)                                                                                                      tCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCAt                                        >  2:374695/1‑151 (MQ=255)                                                                                                       ctggctggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATc                                       >  1:439857/1‑151 (MQ=255)                                                                                                            tggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCg                                   >  2:653531/1‑150 (MQ=255)                                                                                                             ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCg                                   <  1:508671/149‑1 (MQ=255)                                                                                                             ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGAt                                 <  2:549239/151‑1 (MQ=255)                                                                                                             ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGAt                                 <  2:516556/151‑1 (MQ=255)                                                                                                                  cgggcggCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAAtt                            <  1:288163/151‑1 (MQ=255)                                                                                                                  cgggcggCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAAtt                            >  2:648249/1‑151 (MQ=255)                                                                                                                  cgggcggCAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAAtt                            >  2:325757/1‑151 (MQ=255)                                                                                                                    ggcggcAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCt                          <  1:346189/151‑1 (MQ=255)                                                                                                                         cAAAAGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGcc                     >  1:128924/1‑151 (MQ=255)                                                                                                                          aaaaGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGcct                    <  1:157284/151‑2 (MQ=255)                                                                                                                          aaaaGCGCCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGccc                    <  2:303957/151‑1 (MQ=255)                                                                                                                                gcCAACATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTaa              <  2:310604/151‑1 (MQ=255)                                                                                                                                   aaCATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAAc           >  1:425621/1‑151 (MQ=255)                                                                                                                                   aaCATCGTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAAc           >  2:667647/1‑151 (MQ=255)                                                                                                                                         gTTTTCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAgg     <  2:407212/151‑1 (MQ=255)                                                                                                                                           tttCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGgcg   >  1:259605/1‑151 (MQ=255)                                                                                                                                            ttCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGgcgc  >  1:259011/1‑151 (MQ=255)                                                                                                                                             tCGCTGGCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGgcgc  >  1:396105/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                GCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGC  >  NC_000913/1363432‑1363721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑