Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,456	T→C	M1M (ATG→GTG) †	ydaG ←	Rac prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,456	0	T	C	100.0%	165.8 / NA	44	M1M (ATG→GTG) †	ydaG	Rac prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

ATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTG  >  NC_000913/1419310‑1419599                                                                                                                                                   |                                                                                                                                                aTTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTa                                                                                                                                             <  1:291770/151‑1 (MQ=255)     tGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTAtctc                                                                                                                                         >  2:204259/1‑151 (MQ=255)      gCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTAtctct                                                                                                                                        >  2:296982/1‑151 (MQ=255)         aaTCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTccc                                                                                                                                     >  2:410711/1‑151 (MQ=255)               acaTCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAAc                                                                                                                               <  2:472211/151‑1 (MQ=255)                caTCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACg                                                                                                                              <  1:210291/151‑1 (MQ=255)                       ttgCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACg                                                                                                                              >  2:532934/1‑144 (MQ=255)                       ttgCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACg                                                                                                                              <  1:532931/144‑1 (MQ=255)                             cGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGa                                                                                                                  >  2:333836/1‑150 (MQ=255)                                ataGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGaact                                                                                                               <  1:130852/150‑1 (MQ=255)                                ataGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGaact                                                                                                               <  2:225301/150‑1 (MQ=255)                                ataGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGaact                                                                                                               >  1:456701/1‑150 (MQ=255)                                ataGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGaact                                                                                                               <  1:410706/150‑1 (MQ=255)                                          ctgcctgcCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGctgcac                                                                                                    >  1:354508/1‑151 (MQ=255)                                            gcctgccAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGctgcactg                                                                                                  >  2:457819/1‑151 (MQ=255)                                            gcctgccAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGctgca                                                                                                     <  2:600018/148‑1 (MQ=255)                                             cctgccAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGctgcactgc                                                                                                 >  1:109066/1‑151 (MQ=255)                                                   agagATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAAtt                                                                                           >  2:278556/1‑151 (MQ=255)                                                      gaTCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGa                                                                                        <  2:40846/151‑1 (MQ=255)                                                       aTCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGaa                                                                                       <  1:445293/151‑1 (MQ=255)                                                                 ttGCTGCGAAAAGCCTGCACAGCCGGGTTGTAAGTGATCCCCAAAAAACCCATAAAATACTGAACTACTTCAAAATGCACCACCTTATCTCTCCCCTTACCGCCGGTTGGCGAAACTACCTGCTGCACTGCAAAATTTGATTCCCGCCGt                                                                              <  1:333833/150‑1 (MQ=255)                                                                  tgctgcGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTc                                                                             >  2:177260/1‑150 (MQ=255)                                                                      gcGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATg                                                                          <  2:150917/149‑1 (MQ=255)                                                                      gcGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGtt                                                                        <  2:650461/151‑1 (MQ=255)                                                                       cGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTc                                                                       >  2:63746/1‑151 (MQ=255)                                                                                  cacaGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCgg                                                            <  2:422855/151‑1 (MQ=255)                                                                                       ccTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGggctgg                                                       >  2:182550/1‑151 (MQ=255)                                                                                          ggTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTa                                                    <  1:296980/151‑1 (MQ=255)                                                                                             tGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACtt                                                 >  1:604237/1‑151 (MQ=255)                                                                                               tAAGTGATACCGCAACAATCCATAAAATACTGATCTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACtt                                                 <  1:286208/149‑1 (MQ=255)                                                                                                     aTACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCtt                                         >  1:70880/1‑151 (MQ=255)                                                                                                           caacaaTCCATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACt                                   <  2:211379/151‑1 (MQ=255)                                                                                                                  ccATCAAATACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGt                            <  2:226822/151‑1 (MQ=255)                                                                                                                       aaaTACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTc                       >  1:109194/1‑151 (MQ=255)                                                                                                                         aTACTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGa                     <  1:28509/151‑1 (MQ=255)                                                                                                                           aCTGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGaa                    <  1:457815/150‑1 (MQ=255)                                                                                                                             tGAACTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGt                  >  2:318943/1‑150 (MQ=255)                                                                                                                               aaCTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTAtt               >  1:513962/1‑151 (MQ=255)                                                                                                                               aaCTACTTCGTAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTAtt               <  1:17122/151‑1 (MQ=255)                                                                                                                                        gTAATGCACCACCTTATCTCTCCCCTCAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAATATTTGCATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGAATTGCCCGGCGt      >  1:174361/1‑151 (MQ=255)                                                                                                                                         tAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGtt     >  2:588612/1‑151 (MQ=255)                                                                                                                                         tAATGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGtt     >  1:142156/1‑151 (MQ=255)                                                                                                                                           aTGCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCt   <  2:132781/151‑1 (MQ=255)                                                                                                                                             gCACCACCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCtg  <  1:312273/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                ATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGGGTGGCGGAACTAACTGCTGCACTGCAAAATTTGAATCCCGCCGTCATGTTCATACGCCTCGGGCTGGCTACTTAACCCCTTACCACTGCCTGGTAACTCGAAGTATTGCCCGGCGTTCTG  >  NC_000913/1419310‑1419599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑