Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,185	G→A	G87S (GGC→AGC)	paaA →	ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,185	0	G	A	100.0%	133.8 / NA	35	G87S (GGC→AGC)	paaA	ring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/18);  total (17/18)

CATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAAT  >  NC_000913/1454038‑1454322                                                                                                                                                    |                                                                                                                                          caTGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCt                                                                                                                                        <  1:332067/151‑1 (MQ=255)     cgcACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTgcgc                                                                                                                                    >  2:449484/1‑151 (MQ=255)            cGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATCCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAgaga                                                                                                                              >  2:20325/1‑150 (MQ=255)             gagaTTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGagaag                                                                                                                            <  2:146154/151‑1 (MQ=255)                  tgttgGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATc                                                                                                                       >  2:221707/1‑151 (MQ=255)                     tgGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTa                                                                                                                     <  2:362227/150‑1 (MQ=255)                     tgGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTa                                                                                                                     <  2:179513/150‑1 (MQ=255)                                     ggTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacg                                                                                                    <  1:400088/151‑1 (MQ=255)                                      gTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATg                                                                                                           <  2:641641/143‑1 (MQ=255)                                      gTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATg                                                                                                           >  1:641631/1‑143 (MQ=255)                                        aaCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacggac                                                                                                 >  1:513294/1‑151 (MQ=255)                                        aaCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacgga                                                                                                  <  1:197537/150‑1 (MQ=255)                                        aaCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacgga                                                                                                  <  1:570880/150‑1 (MQ=255)                                         aCTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCgacggacg                                                                                                <  1:403094/151‑1 (MQ=255)                                              aTCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGa                                                                                           <  1:253303/151‑1 (MQ=255)                                                      cgcACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATtcc                                                                                   >  1:489229/1‑151 (MQ=255)                                                            aaCCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATc                                                                             >  1:48465/1‑151 (MQ=255)                                                                 tGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTa                                                                         >  1:492561/1‑150 (MQ=255)                                                                 tGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTa                                                                         <  1:562369/150‑1 (MQ=255)                                                                               aTTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTg                                                           >  1:304048/1‑150 (MQ=255)                                                                                 tCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAg                                                         <  1:151868/150‑1 (MQ=255)                                                                                    gTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTg                                                      <  1:449479/150‑1 (MQ=255)                                                                                           aaaGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGata                                              <  1:20326/151‑1 (MQ=255)                                                                                           aaaGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGat                                               >  1:602652/1‑150 (MQ=255)                                                                                                             ggTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCggctggc                            >  1:107164/1‑151 (MQ=255)                                                                                                               tCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGgctggctg                          >  1:37754/1‑151 (MQ=255)                                                                                                                 aTGGTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGctggctgg                         >  2:547571/1‑150 (MQ=255)                                                                                                                   ggTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCtggtgg                      >  1:395854/1‑151 (MQ=255)                                                                                                                   ggTCTGTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCtggtg                       <  1:522578/150‑1 (MQ=255)                                                                                                                        gTATCTCTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATgg                  <  2:199083/150‑1 (MQ=255)                                                                                                                           tctcTATAGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGgcgc               >  1:526997/1‑150 (MQ=255)                                                                                                                                ataGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGc            <  1:516517/148‑1 (MQ=255)                                                                                                                                 taGCGCCGCCGAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATc        >  1:461097/1‑151 (MQ=255)                                                                                                                                       cgccgAAACACTGAGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAAt  >  2:326627/1‑151 (MQ=255)                                                                                                                                           gAAACTCTGAGCAGCGACAGAGAAGTCATCTACCAAAAAATGCTCGACGGAGGGGTGAGATGTTCCTCGTTCTTTAGTTATCCGGCAGTGAGGTGGGCTGATATCAGTGTTATCGGCTGGCTGGTGGATGGCCTAGTGATCGt      <  1:156101/143‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                          CATGCGCACTCCGAGATTGTTGGTATGTTGCCTGAAGGTAACTGGATCACTCGCGCACCAACCTTGCGGCGTAAAGCCATTCTGTTGGCCAAAGTGCAGGATGAAGCCGGTCATGGTCTGTATCTCTATAGCGCCGCCGAAACACTGGGCTGCGCCAGAGAAGACATCTACCAAAAAATGCTCGACGGACGGATGAAATATTCCTCCATCTTTAATTATCCGACATTGAGTTGGGCTGATATCGGTGTTATCGGCTGGCTGGTGGATGGCGCAGCGATCGTCAAT  >  NC_000913/1454038‑1454322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑