Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,483,905	T→C	V282A (GTA→GCA)	hrpA →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,483,905	0	T	C	100.0%	111.3 / NA	35	V282A (GTA→GCA)	hrpA	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

CCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACA  >  NC_000913/1483760‑1484048                                                                                                                                                  |                                                                                                                                                ccGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACg                                                                                                                                            >  2:504022/1‑151 (MQ=255)         cTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGt                                                                                                                                    >  2:510514/1‑151 (MQ=255)                       ttAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATg                                                                                                                       <  1:646843/150‑1 (MQ=255)                         aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGc                                                                                                                     <  1:181999/150‑1 (MQ=255)                         aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCg                                                                                                                    <  1:376975/151‑1 (MQ=255)                         aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCg                                                                                                                    <  1:619608/151‑1 (MQ=255)                         aataatGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGGGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCg                                                                                                                    >  1:135010/1‑151 (MQ=255)                               gcgcCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTc                                                                                                              >  2:555509/1‑151 (MQ=255)                                       tattGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCttt                                                                                                      >  1:216950/1‑151 (MQ=255)                                         ttGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTAt                                                                                                    >  1:261309/1‑151 (MQ=255)                                            aaGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATg                                                                                                   >  1:155289/1‑149 (MQ=255)                                            aaGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAg                                                                                                 <  1:436868/151‑1 (MQ=255)                                                                        aGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATAccgcc                                                                     <  2:434093/151‑1 (MQ=255)                                                                              cTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgc                                                                >  2:458359/1‑150 (MQ=255)                                                                               tATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgcgc                                                              >  2:636930/1‑151 (MQ=255)                                                                                aTCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATgcg                                                               <  2:384609/149‑1 (MQ=255)                                                                                aTCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCt                                                             <  2:314940/151‑1 (MQ=255)                                                                                   gCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGa                                                           >  1:483378/1‑150 (MQ=255)                                                                                    cccGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAAc                                                         <  2:155292/151‑1 (MQ=255)                                                                                     ccGATTGTTGAAGAAGCCGATGACCCCGGGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTAGGAGCGGCGAGCGGAAAATCCGCGATACCGCCGATGCGCTGAACa                                                        <  1:251062/151‑1 (MQ=255)                                                                                         ttgttgAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCt                                                    >  2:177323/1‑151 (MQ=255)                                                                                                    gCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGc                                          <  2:621479/150‑1 (MQ=255)                                                                                                              ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGaaa                                <  1:579237/150‑1 (MQ=255)                                                                                                              ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAAt                               <  2:657541/151‑1 (MQ=255)                                                                                                              ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAAt                               <  2:216951/151‑1 (MQ=255)                                                                                                              ccGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAAt                               <  1:510511/151‑1 (MQ=255)                                                                                                                    gcgATCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGcc                         >  2:373078/1‑151 (MQ=255)                                                                                                                       aTCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGc                       <  1:396604/150‑1 (MQ=255)                                                                                                                       aTCAGTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCt                      <  1:555504/151‑1 (MQ=255)                                                                                                                           gTTGCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTAt                  >  2:389782/1‑151 (MQ=255)                                                                                                                              gCAGGCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATgcg               >  1:218340/1‑151 (MQ=255)                                                                                                                                  gCGATTTTTGACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGc           >  2:317329/1‑151 (MQ=255)                                                                                                                                           gACGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACa  <  2:270584/151‑1 (MQ=255)                                                                                                                                            aCGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGCAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAAc   >  1:39432/1‑149 (MQ=255)                                                                                                                                            aCGCCGCAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAAc   >  1:206688/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                CCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTATCGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGACGCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAGCGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACA  >  NC_000913/1483760‑1484048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑