Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,602	T→C	Y330C (TAC→TGC)	narZ ←	nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,602	0	T	C	100.0%	103.9 / NA	32	Y330C (TAC→TGC)	narZ	nitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

ACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTA  >  NC_000913/1541455‑1541748                                                                                                                                                    |                                                                                                                                                   aCTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCagcagt                                                                                                                                                 <  2:502730/151‑1 (MQ=255)    gCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGtt                                                                                                                                                <  1:453203/149‑1 (MQ=255)       aCGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATaa                                                                                                                                           <  2:324409/151‑1 (MQ=255)       aCGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATa                                                                                                                                            <  1:578022/150‑1 (MQ=255)          gTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATaaa                                                                                                                                          >  1:117475/1‑149 (MQ=255)          gTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAag                                                                                                                                         >  1:26867/1‑150 (MQ=255)            tttCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCCGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCGGTTGATaaa                                                                                                                                          >  1:75290/1‑147 (MQ=255)               ccACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTc                                                                                                                                    <  2:66048/150‑1 (MQ=255)               ccACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTc                                                                                                                                    <  2:273389/150‑1 (MQ=255)               ccACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCg                                                                                                                                   <  1:546854/151‑1 (MQ=255)                cACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGc                                                                                                                                  >  1:397180/1‑151 (MQ=255)                      ggATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCCGTTGGTAAAGGAGTCGATggg                                                                                                                              >  1:325846/1‑149 (MQ=255)                           gttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCg                                                                                                                       <  1:559838/151‑1 (MQ=255)                           gttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCg                                                                                                                       <  1:603625/151‑1 (MQ=255)                            ttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATc                                                                                                                        <  2:453604/149‑1 (MQ=255)                                        aGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCtt                                                                                                          >  2:134084/1‑151 (MQ=255)                                           ccatccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTa                                                                                                       <  1:122640/151‑1 (MQ=255)                                               ccaccaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAAt                                                                                                   >  2:667388/1‑151 (MQ=255)                                                 accaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATc                                                                                                  >  2:618975/1‑150 (MQ=255)                                                    aGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGGTAAAGGAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACAt                                                                                              >  1:394204/1‑151 (MQ=255)                                                               gcgGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGcc                                                                                   >  1:633853/1‑151 (MQ=255)                                                                  gATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATc                                                                                <  2:492294/151‑1 (MQ=255)                                                                              gggAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATAGCCCGGgcgc                                                                      >  1:669130/1‑149 (MQ=255)                                                                                    gTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGAAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGt                                                               >  2:288023/1‑150 (MQ=255)                                                                                       gCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCt                                                           >  2:212341/1‑151 (MQ=255)                                                                                             aTCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTc                                                     <  1:608116/151‑1 (MQ=255)                                                                                                  tgcgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCCGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTg                                                 >  1:148799/2‑150 (MQ=255)                                                                                                      aGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgccagc                                            >  1:506499/1‑151 (MQ=255)                                                                                                           ccAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTg                                       >  1:204284/1‑151 (MQ=255)                                                                                                                                    gCTGTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCagag              <  1:68996/151‑1 (MQ=255)                                                                                                                                       gTAGCGGCGGCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGagta            <  2:524010/150‑1 (MQ=255)                                                                                                                                           cggcggCAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAg       <  1:490876/151‑1 (MQ=255)                                                                                                                                               ggcAGCAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGt   <  2:633863/151‑1 (MQ=255)                                                                                                                                                gcagcaGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTa  <  2:371900/151‑1 (MQ=255)                                                                                                                                                gcagcaGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTa  <  2:512961/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ACTGCTACGGTTTTCCACTGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTA  >  NC_000913/1541455‑1541748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑