Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,630,461	A→G	N151N (AAT→AAC)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,630,461	0	A	G	100.0%	157.6 / NA	45	N151N (AAT→AAC)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/21);  total (24/21)

GCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTA  >  NC_000913/1630315‑1630600                                                                                                                                                   |                                                                                                                                            gCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGtttt                                                                                                                                         <  2:117384/151‑1 (MQ=255) gCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGtttt                                                                                                                                         <  1:365749/151‑1 (MQ=255)       gAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGt                                                                                                                                   <  2:547960/151‑1 (MQ=255)               aaCGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCt                                                                                                                           <  2:336972/151‑1 (MQ=255)               aaCGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCt                                                                                                                           >  1:42008/1‑151 (MQ=255)                        cATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCggg                                                                                                                  >  2:393365/1‑151 (MQ=255)                        cATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCggg                                                                                                                  <  1:550775/151‑1 (MQ=255)                             cATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATc                                                                                                             >  1:237581/1‑151 (MQ=255)                                gACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCg                                                                                                            >  2:125235/1‑149 (MQ=255)                                    gTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAtt                                                                                                      <  1:283843/151‑1 (MQ=255)                                       ttCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAAc                                                                                                   >  1:346339/1‑151 (MQ=255)                                         cTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACt                                                                                                  >  1:247983/1‑150 (MQ=255)                                             cATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCg                                                                                             <  1:301546/151‑1 (MQ=255)                                                gttgtCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTGCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGt                                                                                           <  1:564658/150‑1 (MQ=255)                                                      acatgacatgACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCgg                                                                                     >  1:300666/1‑150 (MQ=255)                                                       catgacatgaCGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGGGGGGGTTTTGCACGCCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCgg                                                                                     >  2:253174/1‑149 (MQ=255)                                                         tgacatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGgag                                                                                   >  2:339374/1‑149 (MQ=255)                                                           acatgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTg                                                                               >  2:538097/1‑151 (MQ=255)                                                            catgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTgg                                                                              <  1:364129/151‑1 (MQ=255)                                                             atgacGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTgg                                                                              >  2:29420/1‑150 (MQ=255)                                                                   gTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAcc                                                                       <  1:233659/151‑1 (MQ=255)                                                                         gCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCtttt                                                                  <  1:584515/150‑1 (MQ=255)                                                                          cGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTc                                                                >  2:350666/1‑151 (MQ=255)                                                                           gggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCg                                                               <  2:264260/151‑1 (MQ=255)                                                                             gAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGt                                                             >  1:557710/1‑151 (MQ=255)                                                                                      ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCag                                                    <  2:192276/151‑1 (MQ=255)                                                                                       cGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTc                                                      >  2:627172/1‑148 (MQ=255)                                                                                       agCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGt                                                       >  1:607980/2‑147 (MQ=255)                                                                                         cTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCag                                                    >  1:558959/1‑148 (MQ=255)                                                                                             aCGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACa                                             <  2:346342/151‑1 (MQ=255)                                                                                                gCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAAt                                           <  2:463959/150‑1 (MQ=255)                                                                                                    gCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcgcg                                       >  1:602548/1‑150 (MQ=255)                                                                                                     ccAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAt                                     <  2:642233/151‑1 (MQ=255)                                                                                                      cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAtt                                    >  2:88792/1‑151 (MQ=255)                                                                                                        gcgcCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg                                  <  1:578062/151‑1 (MQ=255)                                                                                                              caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTc                            <  2:654851/151‑1 (MQ=255)                                                                                                               aacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTc                            >  2:38445/1‑150 (MQ=255)                                                                                                                acaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTcac                          <  2:620051/151‑1 (MQ=255)                                                                                                                       ccccTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCg                    <  1:125232/150‑1 (MQ=255)                                                                                                                       ccccTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGc                   >  1:599081/1‑151 (MQ=255)                                                                                                                          cTGTTGCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGc                >  1:605820/1‑151 (MQ=255)                                                                                                                               gCTGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAac            >  2:132169/1‑150 (MQ=255)                                                                                                                                 tGTTTTCGGCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg         >  1:484690/1‑151 (MQ=255)                                                                                                                                 tGTTTTCGGCTGGGGGGGGTTTTGCACGTCGGCGGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg         >  1:62104/1‑151 (MQ=255)                                                                                                                                         gCTGGTGGGGGTTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGGATTAACTGTCCGGTCGCCGGAGAGTTGAAATACCCTTTTTCGGTGATTGTCAGATAGACAATCGCGATTTGCTGTTCCCACATCGCTGCCAACACGGTTTCTa  <  1:106660/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            GCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTA  >  NC_000913/1630315‑1630600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑