Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,590	T→C	V298A (GTT→GCT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,590	0	T	C	100.0%	188.9 / NA	50	V298A (GTT→GCT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

GTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACA  >  NC_000913/1756442‑1756736                                                                                                                                                     |                                                                                                                                                   gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTg                                                                                                                                                  >  2:123068/1‑151 (MQ=255)  tAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGc                                                                                                                                                 >  2:153749/1‑151 (MQ=255)          ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGcc                                                                                                                                          >  1:297985/1‑150 (MQ=255)                gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCg                                                                                                                                   <  2:529431/151‑1 (MQ=255)                  agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGAc                                                                                                                                 <  2:278727/151‑1 (MQ=255)                    aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACCCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACgg                                                                                                                               <  1:261389/151‑1 (MQ=255)                         aTCTTCGCACAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTg                                                                                                                          >  2:368003/1‑151 (MQ=255)                               gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGc                                                                                                                      >  1:189283/1‑149 (MQ=255)                                      agaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCt                                                                                                             >  2:16746/1‑151 (MQ=255)                                          gatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc                                                                                                          <  1:469171/150‑1 (MQ=255)                                          gatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCt                                                                                                         >  2:380588/1‑151 (MQ=255)                                              atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATCCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTg                                                                                                     >  2:128945/1‑151 (MQ=255)                                                 gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAAt                                                                                                  <  1:661566/151‑1 (MQ=255)                                                  aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATc                                                                                                 <  1:411133/151‑1 (MQ=255)                                                  aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATc                                                                                                 <  1:601714/151‑1 (MQ=255)                                                   aaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATcc                                                                                                <  2:6747/151‑1 (MQ=255)                                                       tGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCa                                                                                             >  2:226485/1‑150 (MQ=255)                                                              gTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTa                                                                                      >  1:643031/1‑150 (MQ=255)                                                               tGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTaa                                                                                     >  2:582660/1‑150 (MQ=255)                                                                gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAAt                                                                                   >  2:87235/1‑151 (MQ=255)                                                                gCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATCCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTaaa                                                                                    <  1:128941/150‑1 (MQ=255)                                                                     tAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCg                                                                              >  1:393659/1‑151 (MQ=255)                                                                      aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGc                                                                             >  2:308210/1‑151 (MQ=255)                                                                           cGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGa                                                                         <  1:582367/150‑1 (MQ=255)                                                                            gTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAg                                                                       <  1:422703/151‑1 (MQ=255)                                                                                  aCTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGttt                                                                 >  2:1179/1‑151 (MQ=255)                                                                                    tGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCt                                                               >  2:6118/1‑151 (MQ=255)                                                                                           cAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATgg                                                        >  1:668178/1‑151 (MQ=255)                                                                                           cAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATgg                                                        >  2:504082/1‑151 (MQ=255)                                                                                             gATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCg                                                      >  2:9789/1‑151 (MQ=255)                                                                                                   ggATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCAt                                                 <  2:420357/150‑1 (MQ=255)                                                                                                    gATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCt                                               <  2:460556/151‑1 (MQ=255)                                                                                                    gATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCt                                               >  2:316964/1‑151 (MQ=255)                                                                                                       tCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGc                                             <  1:118249/150‑1 (MQ=255)                                                                                                       tCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCg                                            >  2:363967/1‑151 (MQ=255)                                                                                                        ccATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGa                                           <  1:110293/151‑1 (MQ=255)                                                                                                          aTGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAAc                                         <  2:363817/151‑1 (MQ=255)                                                                                                          aTGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAAc                                         <  2:131026/151‑1 (MQ=255)                                                                                                          aTGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAAc                                         <  1:264079/151‑1 (MQ=255)                                                                                                            gATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACg                                        >  1:481442/1‑150 (MQ=255)                                                                                                               cAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTacc                                    >  2:261023/1‑151 (MQ=255)                                                                                                                   aaCCCACGCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTaccgacc                                <  1:587483/151‑1 (MQ=255)                                                                                                                          gCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                         >  2:368651/1‑151 (MQ=255)                                                                                                                          gCCCGACTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                         >  1:172402/1‑151 (MQ=255)                                                                                                                               aCTCGCGCAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACa                    <  1:368647/151‑1 (MQ=255)                                                                                                                                   gcgcAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCg                >  1:125893/1‑151 (MQ=255)                                                                                                                                    cgcAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGt               >  2:527925/1‑151 (MQ=255)                                                                                                                                      cAGAAGCCGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtct             <  1:547016/151‑1 (MQ=255)                                                                                                                                             cGGTGACGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTc      <  1:198522/151‑1 (MQ=255)                                                                                                                                                  aCGCTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGAGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaaca  >  2:137841/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACA  >  NC_000913/1756442‑1756736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑