Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,078	T→C	intergenic (‑58/+189)	ydiV ← / ← nlpC	anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,078	0	T	C	100.0%	129.2 / NA	36	intergenic (‑58/+189)	ydiV/nlpC	anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/14);  total (22/14)

ACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGG  >  NC_000913/1791933‑1792221                                                                                                                                                  |                                                                                                                                                 aCTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGAc                                                                                                                                             >  1:475647/1‑151 (MQ=255)  cTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGAc                                                                                                                                             >  1:328443/1‑150 (MQ=255)    cAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACAt                                                                                                                                           >  2:388389/1‑150 (MQ=255)        gCCGACAAGAACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCATCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACaa                                                                                                                                      <  1:529365/151‑1 (MQ=255)          cGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAAtt                                                                                                                                    <  2:150787/151‑1 (MQ=255)          cGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAAtt                                                                                                                                    >  1:616891/1‑151 (MQ=255)              aaGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTAc                                                                                                                                  >  2:64883/1‑149 (MQ=255)                            tGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACttt                                                                                                                   <  1:212417/150‑1 (MQ=255)                                 ctTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCAtt                                                                                                               <  2:459627/149‑1 (MQ=255)                                  ttATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGt                                                                                                            >  1:316487/1‑151 (MQ=255)                                   tATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGt                                                                                                            <  1:116861/150‑1 (MQ=255)                                          aGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGtt                                                                                                    >  2:636883/1‑151 (MQ=255)                                                tAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCAc                                                                                              >  2:404137/1‑151 (MQ=255)                                                         gAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAgg                                                                                     <  1:602498/151‑1 (MQ=255)                                                             gATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTc                                                                                 >  1:577215/1‑151 (MQ=255)                                                                aaaGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑tt                                                                             <  2:494145/151‑1 (MQ=255)                                                                       ctcCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGATAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑tttttt                                                                         >  1:210675/1‑148 (MQ=255)                                                                         ccAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑ttttttt                                                                        >  2:461670/1‑147 (MQ=255)                                                                          cAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGAtt                                                                   >  2:371776/1‑151 (MQ=255)                                                                             aaaaaTCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATc                                                                >  2:633308/1‑151 (MQ=255)                                                                               aaaTCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCt                                                              >  1:503721/1‑151 (MQ=255)                                                                                     tCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGa                                                            <  2:317416/147‑1 (MQ=255)                                                                                            aaCCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaa                                                 <  1:449171/151‑1 (MQ=255)                                                                                              ccGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaata                                               <  2:328445/151‑1 (MQ=255)                                                                                                gTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaataat                                             >  2:487027/1‑151 (MQ=255)                                                                                                gTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGaataat                                             >  1:369452/1‑151 (MQ=255)                                                                                                   ccTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTTAGATTATCCTGATTATAAACGAATAATcc                                           >  1:179910/1‑151 (MQ=255)                                                                                                   ccTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATc                                            >  1:280928/1‑149 (MQ=255)                                                                                                                gTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAAttt                              <  1:404133/150‑1 (MQ=255)                                                                                                                gTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAAttt                              <  1:210833/150‑1 (MQ=255)                                                                                                                          aTCCGGCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCaa                    >  2:374206/1‑150 (MQ=255)                                                                                                                              ggCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtac               >  2:435906/1‑151 (MQ=255)                                                                                                                               gCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtact              >  2:187189/1‑151 (MQ=255)                                                                                                                               gCTAAAAGGGATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtact              <  1:89705/151‑1 (MQ=255)                                                                                                                                       ggATGAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTAtt      >  1:60852/1‑151 (MQ=255)                                                                                                                                           gAGGCCACAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAgg  <  1:87137/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 ACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCC‑TTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGG  >  NC_000913/1791933‑1792221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑