Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,861,807	T→C	A56A (GCA→GCG)	yeaC ←	DUF1315 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,861,807	0	T	C	100.0%	140.8 / NA	39	A56A (GCA→GCG)	yeaC	DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/21);  total (18/21)

GGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAG  >  NC_000913/1861660‑1861940                                                                                                                                                    |                                                                                                                                      ggCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTgcgc                                                                                                                                     <  2:454607/150‑1 (MQ=255)     aTCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCg                                                                                                                                 <  2:357636/150‑1 (MQ=255)       ccccTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGat                                                                                                                               >  2:381429/1‑150 (MQ=255)         ccTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGAtatt                                                                                                                            >  1:151988/1‑151 (MQ=255)             cATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATg                                                                                                                         <  1:321532/150‑1 (MQ=255)              aTAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGc                                                                                                                       >  1:614309/1‑151 (MQ=255)               tAGCCGAGGCAAAAAGGGTATGAACCACTTACTTAAACATTGCAATAGGCTTTGCTCAGATACCAAAATCTTCTTTTAACTGCTGTTTGTTCTTCATGACCATCTGACCATTTGTGTCAATTTTCATATGCTGCGATTCGAtattat                                                                                                                          <  1:192621/147‑1 (MQ=255)               tAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCg                                                                                                                      >  2:489661/1‑151 (MQ=255)                aGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCgg                                                                                                                     <  1:226615/151‑1 (MQ=255)                aGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCg                                                                                                                      <  1:549584/150‑1 (MQ=255)                   cGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCt                                                                                                                  <  2:234221/151‑1 (MQ=255)                    gAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGGtt                                                                                                                 >  2:138580/1‑151 (MQ=255)                    gAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCtt                                                                                                                 <  1:190803/151‑1 (MQ=255)                    gAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCtt                                                                                                                 <  1:240953/151‑1 (MQ=255)                          aaaaGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCcaca                                                                                                           <  1:186627/151‑1 (MQ=255)                           aaaGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGttttttac                                                                                                            >  1:105925/1‑144 (MQ=255)                                ggATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCa                                                                                                     <  2:339353/151‑1 (MQ=255)                                  aTGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGTCACAGCATCaac                                                                                                   >  2:354378/1‑149 (MQ=255)                                      aCCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGtt                                                                                               <  2:375097/151‑1 (MQ=255)                                                        gCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGTCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCttttt                                                                             <  2:196913/151‑1 (MQ=255)                                                           aTAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGCTGCAGGCAGTTCTCtttttttt                                                                          >  2:49629/1‑151 (MQ=255)                                                                  ttGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTTCCGGCCGTTCTTTTTTTTTTtctc                                                                      >  1:79096/1‑148 (MQ=255)                                                                   tGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGt                                                                   <  2:364553/150‑1 (MQ=255)                                                                    gCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTca                                                                 <  2:283060/151‑1 (MQ=255)                                                                      tGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCCTACCCCGTTGGCGGCAGTTTTCTTTTTTTTTCTCGGTcaa                                                                >  2:10786/1‑150 (MQ=255)                                                                       gagaTACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaac                                                               <  2:251094/150‑1 (MQ=255)                                                                       gagaTACCAAAATCTTCCTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTcaac                                                               <  2:89899/150‑1 (MQ=255)                                                                               aaaaTCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCACCCTCAACCCTTTCGGGCAGTTTTCTTTTTTTTTCTCCGTccaccccacccc                                                       >  1:113452/1‑142 (MQ=255)                                                                                aaaTCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGTTGCAGGCAGGTCTCTTTTTTTTCCTCGGTCAACGCAACGcc                                                       >  2:507331/1‑149 (MQ=255)                                                                                        ttttAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAATTGCAGGCCGTTCTCTTTTTTTTCCCCCGTCAACCCCACCCCCTCAAGcc                                               >  1:136643/1‑149 (MQ=255)                                                                                         tttAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCAt                                             <  1:610135/150‑1 (MQ=255)                                                                                             aCTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGGTTGCCACAGCATCACCCAGTGTAGGGCGGTTTTTTTTTTTTCCTCCGGCCACACAACGCAATCAAGCCACTTtcc                                        >  2:581942/1‑151 (MQ=255)                                                                                                    tttGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGCTGGAGGCAGGTCTTTTTTTTTTTCTCGGTCAACGCAACCCCATCAGGCCATTTCCCCCGtt                                   >  2:385916/1‑149 (MQ=255)                                                                                                     ttGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCa                                 <  2:50325/150‑1 (MQ=255)                                                                                                          ctTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTTCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTTCTCGGGCAACGCAACGCCATCAGGCCATTTCCCCCGTTCAAcggcg                           >  2:16070/1‑151 (MQ=255)                                                                                                              aTGACCATCTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTca                       <  1:526116/151‑2 (MQ=255)                                                                                                                      cTGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTTCTCGGTCAAAGCAACGCCATCAGGCCATTTCCCCACGTCAACGGCGGGCGACaaa                  >  2:4013/1‑148 (MQ=255)                                                                                                                       tGACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCCCAGCATCACCAGTTGCAGGCAGTTCTCTTTTTTTTCCTCGGGCCACGCAACGCCATCCGGCCATTTCCCCAGTTCAACGGCGGGCGCCaaaacc               >  2:26161/1‑147 (MQ=255)                                                                                                                        gACCATTTGTGTCAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTg             <  1:424314/151‑1 (MQ=255)                                                                                                                                    cAATTGTCATATGCTGCGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAg  <  2:115528/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                      GGCTATCCCCTTCATAGCCGAGGCAAAAAGGGGATGAACCACTTACTTAAACATGGCAATAGGCTTTGCTGAGATACCAAAATCTTCTTTTAACTGCTGTTTGCTCTTCATGACCATCTGACCATTTGTGTCAATTGTCATATGCTGTGCTTCGATATTATGGCGGGCTTGCCACAGCATCACCAGTTGCAGGCAGTTCTCTTTTTGTTCCTCGGTCAACGCAACGCCATCAGGCCATTTCCCCAGTTCAACGGCGGTCGACAAACGCTGGTATACCTCAG  >  NC_000913/1861660‑1861940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑