Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,313	T→C	A181A (GCT→GCC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,313	0	T	C	100.0%	125.1 / NA	34	A181A (GCT→GCC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/12);  total (22/12)

AAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCG  >  NC_000913/1863165‑1863460                                                                                                                                                     |                                                                                                                                                    aaGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCAc                                                                                                                                                   >  2:98742/1‑151 (MQ=255)        tAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGaa                                                                                                                                             >  2:560677/1‑150 (MQ=255)         aaCTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGaaa                                                                                                                                            >  1:548093/1‑150 (MQ=255)            ttCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCg                                                                                                                                        >  1:319427/1‑151 (MQ=255)                  aaaTATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGAt                                                                                                                                   <  2:212888/150‑1 (MQ=255)                       tGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCg                                                                                                                             >  2:454639/1‑151 (MQ=255)                                        tttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcg                                                                                                            >  2:300803/1‑151 (MQ=255)                                         ttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcgc                                                                                                           >  2:99234/1‑151 (MQ=255)                                         ttCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGgcgc                                                                                                           >  1:630988/1‑151 (MQ=255)                                            cAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCgcggc                                                                                                        >  2:664816/1‑151 (MQ=255)                                             aaCGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCgcggcg                                                                                                       >  2:28783/1‑151 (MQ=255)                                                  ttCCTGCACCCCCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTGGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGcc                                                                                                    <  2:402351/149‑1 (MQ=255)                                                         ccccccaACTGCCTGGCTCCGCGGGATAAAGTTATCAACTATAACTTGGGCATCATCTAGGTTCTGAGGCCCCCCGTTCCCGCAACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCtt                                                                                           <  2:578667/147‑1 (MQ=255)                                                                aCTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGa                                                                                     >  1:14842/1‑150 (MQ=255)                                                                           ccGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGt                                                                         >  2:392901/1‑151 (MQ=255)                                                                             gCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTcc                                                                       >  2:64673/1‑151 (MQ=255)                                                                                ggCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGCCCACCGTTCCCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCctct                                                                    <  1:392897/151‑1 (MQ=255)                                                                                      aGTTTTCAACGATAAATTCGGCATCATCGAAGGGCTGATGACCACCCTTCACGCTACTACCCCCACCCAGAAAAACGTTGATAGCCCCTCTCACAAAAACTGGCGCGGCGGCCGCCGCGCTTCCCAGAACATCATCCCGCCCTCTACCgg                                                               >  1:409456/1‑150 (MQ=255)                                                                                             aaCGATAACTTCGGCATCATCGAAGGTCTGATGCCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGtgctgc                                                         <  2:37928/149‑1 (MQ=255)                                                                                               cGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTaa                                                      >  2:149930/1‑150 (MQ=255)                                                                                                    aCTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTAACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGt                                                >  1:209119/1‑151 (MQ=255)                                                                                                      ttCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGt                                                >  1:91007/1‑149 (MQ=255)                                                                                                       tCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAgg                                             >  2:99185/1‑151 (MQ=255)                                                                                                                   aaGGTTTGAGGACCCCCGTCCACGTTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACt                                    <  2:91008/148‑1 (MQ=255)                                                                                                                   aaGGTCTGATGACCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGcc                                 >  1:98036/1‑151 (MQ=255)                                                                                                                         tggtccccccgtttCACGCTATTGCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGTCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAg                               <  1:379163/135‑1 (MQ=255)                                                                                                                          gatgcCCACCGTTCACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAAc                            <  1:240654/149‑1 (MQ=255)                                                                                                                                     ttaaccctactcCCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCaaa                 <  1:198606/143‑1 (MQ=255)                                                                                                                                       cACGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactg              >  2:376911/1‑150 (MQ=255)                                                                                                                                        aCGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgac            >  2:234220/1‑151 (MQ=255)                                                                                                                                         cGCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactga             <  2:423367/149‑1 (MQ=255)                                                                                                                                          gCTACTACCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgactg          >  1:357244/1‑151 (MQ=255)                                                                                                                                            tactacCGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgactg          <  2:548098/149‑1 (MQ=255)                                                                                                                                                  cGCCACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGgcg  <  1:98740/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AAGGCGCTAACTTCGACAAATATGCTGGCCAGGACATCGTTTCCAACGCTTCCTGCACCACCAACTGCCTGGCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCG  >  NC_000913/1863165‑1863460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑