Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	240,865	G→A	A813A (GCC→GCT)	fadE ←	acyl coenzyme A dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	240,865	0	G	A	100.0%	218.6 / NA	56	A813A (GCC→GCT)	fadE	acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/31);  total (25/31)

GAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCC  >  NC_000913/240717‑241007                                                                                                                                                     |                                                                                                                                               gAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGc                                                                                                                                              >  2:311302/1‑151 (MQ=255)  aaTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCt                                                                                                                                             <  2:418025/151‑1 (MQ=255)     gtgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCtt                                                                                                                                            <  1:512587/149‑1 (MQ=255)     gtgAACGATGCGCTTTCGATTGATGGTAAAACGGAGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTc                                                                                                                                           >  2:117159/1‑150 (MQ=255)            aTGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTcc                                                                                                                                   <  1:403422/151‑1 (MQ=255)             tGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCg                                                                                                                                  >  2:317233/1‑151 (MQ=255)                    tCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACttt                                                                                                                            <  1:431541/150‑1 (MQ=255)                      gATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTtctc                                                                                                                         >  2:27553/1‑151 (MQ=255)                       aTTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTcc                                                                                                                        >  1:165346/1‑151 (MQ=255)                         tGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCGCTTTCTCCg                                                                                                                       <  1:343320/150‑1 (MQ=255)                                aaaaCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACttt                                                                                                               >  1:461357/1‑151 (MQ=255)                                 aaaCGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACttt                                                                                                               >  1:555972/1‑150 (MQ=255)                                    cGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcct                                                                                                           <  1:509795/151‑2 (MQ=255)                                      gtgtTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGc                                                                                                         >  2:514641/1‑151 (MQ=255)                                        gttgttCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCt                                                                                                        <  1:542683/150‑1 (MQ=255)                                              cgcgGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtcg                                                                                                  <  1:534258/150‑1 (MQ=255)                                              cgcgGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGc                                                                                                 >  2:197045/1‑151 (MQ=255)                                                 ggcgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGcc                                                                                                >  2:498890/1‑149 (MQ=255)                                                         ccGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCg                                                                                      <  1:579681/151‑1 (MQ=255)                                                          cGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCgg                                                                                     <  1:22648/151‑1 (MQ=255)                                                          cGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCgg                                                                                     <  2:638732/151‑1 (MQ=255)                                                             cAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATc                                                                                  <  1:577281/151‑1 (MQ=255)                                                                  tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGt                                                                             >  1:176519/1‑151 (MQ=255)                                                                   ggAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtc                                                                            <  1:113255/151‑1 (MQ=255)                                                                         ccATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAAc                                                                      <  2:513758/151‑1 (MQ=255)                                                                          cATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAAc                                                                      >  1:367293/1‑150 (MQ=255)                                                                           aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGt                                                                    <  2:332442/151‑1 (MQ=255)                                                                            tCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGtt                                                                   >  1:294536/1‑151 (MQ=255)                                                                             ccGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCTTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                                                  <  1:458546/151‑1 (MQ=255)                                                                             ccGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                                                  <  1:514901/151‑1 (MQ=255)                                                                                       ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcg                                                          <  1:333231/149‑1 (MQ=255)                                                                                        tAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                                         >  1:578989/1‑149 (MQ=255)                                                                                         aaTTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAg                                                       <  2:313756/150‑1 (MQ=255)                                                                                              tAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGc                                                  <  1:200776/150‑1 (MQ=255)                                                                                               aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                                         >  1:428252/1‑142 (MQ=255)                                                                                               aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgcgc                                                         <  2:428257/142‑1 (MQ=255)                                                                                                   aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttctt                                            >  2:103215/1‑151 (MQ=255)                                                                                                   aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttct                                             <  2:287825/150‑1 (MQ=255)                                                                                                     ttAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttcttc                                           >  2:350640/1‑150 (MQ=255)                                                                                                      tAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCa                                          <  2:305562/150‑1 (MQ=255)                                                                                                      tAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCa                                          <  1:666427/150‑1 (MQ=255)                                                                                                        gCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCt                                       <  1:119572/151‑1 (MQ=255)                                                                                                          ggATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                     <  2:41792/151‑1 (MQ=255)                                                                                                          ggATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                     <  1:77688/151‑1 (MQ=255)                                                                                                            aTAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCa                                   <  2:511363/151‑1 (MQ=255)                                                                                                                             ttCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCtt                    <  2:88487/149‑1 (MQ=255)                                                                                                                             ttCGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTc                   >  2:427982/1‑150 (MQ=255)                                                                                                                               cGGCTCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                >  2:189760/1‑151 (MQ=255)                                                                                                                                   tCCGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt             >  2:299435/1‑150 (MQ=255)                                                                                                                                     cGTTATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatc          >  2:601445/1‑151 (MQ=255)                                                                                                                                       ttATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatca         >  2:630615/1‑150 (MQ=255)                                                                                                                                        tATTCATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatcaat       >  2:239907/1‑151 (MQ=255)                                                                                                                                            cATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAg    <  1:463096/150‑1 (MQ=255)                                                                                                                                            cATTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGc   >  2:90970/1‑151 (MQ=255)                                                                                                                                             aTTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGcc  <  2:606738/151‑1 (MQ=255)                                                                                                                                             aTTTACGCAGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGcc  >  1:209547/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                               GAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCC  >  NC_000913/240717‑241007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑