Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,936,375	A→G	intergenic (‑61/‑277)	zwf ← / → yebK	glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,375	0	A	G	100.0%	136.4 / NA	37	intergenic (‑61/‑277)	zwf/yebK	glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/12);  total (25/12)

AAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTT  >  NC_000913/1936230‑1936521                                                                                                                                                  |                                                                                                                                                   aaGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTg                                                                                                                                               >  2:100212/1‑151 (MQ=255)       gcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTg                                                                                                                                          <  1:530236/150‑1 (MQ=255)                  cgcgCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTa                                                                                                                               <  1:476363/150‑1 (MQ=255)                   gcgcAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACg                                                                                                                             >  1:270028/1‑151 (MQ=255)                   gcgcAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACg                                                                                                                             >  2:583981/1‑151 (MQ=255)                            cGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCg                                                                                                                     >  1:639320/1‑150 (MQ=255)                            cGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCg                                                                                                                     >  2:481061/1‑150 (MQ=255)                                                aCCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCa                                                                                                <  1:670481/151‑1 (MQ=255)                                                              cTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACg                                                                                  >  1:56745/1‑151 (MQ=255)                                                                 ggCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACggg                                                                                >  2:603843/1‑150 (MQ=255)                                                                       ttGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAgc                                                                          <  1:204046/150‑1 (MQ=255)                                                                        tGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAgcgc                                                                        >  2:518955/1‑151 (MQ=255)                                                                           gTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAgcgcgc                                                                      >  2:224555/1‑150 (MQ=255)                                                                                 gCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGc                                                               >  2:23136/1‑151 (MQ=255)                                                                                   cATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCgg                                                             >  1:340256/1‑151 (MQ=255)                                                                                          ttCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACt                                                      >  2:39338/1‑151 (MQ=255)                                                                                                   aGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATaaaaa                                              >  1:343830/1‑150 (MQ=255)                                                                                                       aactaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAatca                                         <  1:603837/151‑1 (MQ=255)                                                                                                       aactaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAatca                                         <  1:205979/151‑1 (MQ=255)                                                                                                        actaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAatcat                                        <  1:323269/151‑1 (MQ=255)                                                                                                        actaacCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAatcat                                        >  2:158388/1‑151 (MQ=255)                                                                                                             cccGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGttt                                   >  2:151896/1‑151 (MQ=255)                                                                                                              ccGGTTCTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGtttt                                  <  1:126691/151‑1 (MQ=255)                                                                                                              ccGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGc                                                               <  1:526199/122‑1 (MQ=255)                                                                                                              ccGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGc                                                               >  2:526202/1‑122 (MQ=255)                                                                                                              ccGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGtttt                                  >  1:278530/1‑151 (MQ=255)                                                                                                                      tAAGCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTg                            <  2:645809/149‑1 (MQ=255)                                                                                                                         gCCAGGGTATACTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTa                        >  1:60581/1‑150 (MQ=255)                                                                                                                                 ataCTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTg                >  2:421572/1‑150 (MQ=255)                                                                                                                                    cTTGTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGacac            >  2:515907/1‑151 (MQ=255)                                                                                                                                       gTAATTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTg          >  2:478060/1‑150 (MQ=255)                                                                                                                                         aaTTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATc       <  1:191230/151‑1 (MQ=255)                                                                                                                                         aaTTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATc       >  2:222824/1‑151 (MQ=255)                                                                                                                                          aTTTTCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATc       >  1:310619/1‑150 (MQ=255)                                                                                                                                           ttttCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCAt     >  1:201824/1‑151 (MQ=255)                                                                                                                                             ttCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATg    <  1:64697/150‑1 (MQ=255)                                                                                                                                              tCTTGCGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGtt  >  2:115259/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTT  >  NC_000913/1936230‑1936521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑