Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,956,520	T→C	N163S (AAC→AGC)	torZ ←	trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,956,520	0	T	C	100.0%	144.8 / NA	39	N163S (AAC→AGC)	torZ	trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/22);  total (17/22)

CAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTCCCA  >  NC_000913/1956372‑1956665                                                                                                                                                     |                                                                                                                                                  caGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTc                                                                                                                                                 >  2:537252/1‑151 (MQ=255)          aaCCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGt                                                                                                                                        <  2:433267/151‑1 (MQ=255)            ccTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGtt                                                                                                                                       <  2:322057/150‑1 (MQ=255)                   gTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTa                                                                                                                                 <  1:522740/149‑1 (MQ=255)                      ttCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGtt                                                                                                                            >  2:210691/1‑151 (MQ=255)                             aTCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCtt                                                                                                                     <  2:38314/151‑1 (MQ=255)                              tCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCttt                                                                                                                    >  2:612981/1‑151 (MQ=255)                               cAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTa                                                                                                                   <  1:480389/151‑1 (MQ=255)                                  cGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGc                                                                                                                >  2:370876/1‑151 (MQ=255)                                      cAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAaca                                                                                                            <  2:534391/151‑1 (MQ=255)                                         gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGc                                                                                                         >  1:117782/1‑151 (MQ=255)                                         gaggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGc                                                                                                         >  1:55604/1‑151 (MQ=255)                                           ggTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGcc                                                                                                        >  2:541938/1‑150 (MQ=255)                                            gTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGcc                                                                                                        >  2:287685/1‑149 (MQ=255)                                                     tCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGcc                                                                                             >  1:519154/1‑151 (MQ=255)                                                            ccTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTa                                                                                      <  1:237612/151‑1 (MQ=255)                                                                     aaCCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGc                                                                             >  2:276040/1‑151 (MQ=255)                                                                       ccGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCa                                                                            >  1:503745/1‑150 (MQ=255)                                                                       ccGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCa                                                                            <  2:32641/150‑1 (MQ=255)                                                                           ccACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCaaaaa                                                                        <  1:399594/150‑1 (MQ=255)                                                                           ccACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAAt                                                                       >  2:224028/1‑151 (MQ=255)                                                                              caTGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATgg                                                                     <  1:210690/150‑1 (MQ=255)                                                                                 gCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGcc                                                                   <  1:87821/149‑1 (MQ=255)                                                                                           cACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGtt                                                        <  1:52833/150‑1 (MQ=255)                                                                                           cACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGtt                                                        <  2:468442/150‑1 (MQ=255)                                                                                           cACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTa                                                       <  2:434860/151‑1 (MQ=255)                                                                                                   ctgcCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACg                                               >  2:41377/1‑151 (MQ=255)                                                                                                     gcCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGa                                              <  1:146797/150‑1 (MQ=255)                                                                                                            ttGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATc                                      >  2:39685/1‑151 (MQ=255)                                                                                                                          cTATGCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGa                         >  1:319314/1‑150 (MQ=255)                                                                                                                              gCCCGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTT‑CGAATACGATCATGTTGCTCATGAAt                       <  2:425382/147‑1 (MQ=255)                                                                                                                               cccGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGt                   <  2:520405/151‑1 (MQ=255)                                                                                                                                ccGGAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGtt                  <  2:654028/151‑1 (MQ=255)                                                                                                                                  ggAATAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGttt                 <  1:252540/150‑1 (MQ=255)                                                                                                                                      tAACCGCCCGCCAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATg            >  2:484659/1‑151 (MQ=255)                                                                                                                                          cgcccgccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTg        <  2:357788/151‑1 (MQ=255)                                                                                                                                            cccgccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGtt      <  1:28766/151‑1 (MQ=255)                                                                                                                                                ccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTccc   >  1:609273/1‑150 (MQ=255)                                                                                                                                                ccAGGCTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTCCCa  <  1:224028/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAGCACCACAACCTGGCTGTTTTCCAGAATCAGCGGCCAGGAGGTCTGCTGTTCATACACCTCTACAGAACCGACCACATGCGGCATGATCACCTGTGCTGCCCCGGTTGAATAATCGCCGCTATGCCCGGAATAACCGCCCGCCAGGTTCATATAACGTTGCAGTAAGGTTTGCGCTTTATGCAACACGCCAGAAGAGCGCCAGCCGTAAGATCCGGCAAAAATGGCCGATGGTCCGTTAGCTTTACGAATACGATCATGTTGCTCATGAATCAGTTTTAATGCCTGTTCCCA  >  NC_000913/1956372‑1956665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑