Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,974,562	T→C	T255A (ACG→GCG)	cheA ←	fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,974,562	0	T	C	100.0%	124.5 / NA	36	T255A (ACG→GCG)	cheA	fused chemotactic sensory histidine kinase in two‑component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

CATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATC  >  NC_000913/1974424‑1974709                                                                                                                                           |                                                                                                                                                    cATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTc                                                                                                                                         >  2:188512/1‑151 (MQ=255)       tAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCAcgcg                                                                                                                                   >  1:574662/1‑151 (MQ=255)          ccGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGcc                                                                                                                                <  2:224594/151‑1 (MQ=255)                          ggAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTc                                                                                                                 <  1:501331/150‑1 (MQ=255)                            aaCGATGGGCACGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGGGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGc                                                                                                              >  1:529182/1‑151 (MQ=255)                                   ggCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGt                                                                                                       <  2:456310/151‑1 (MQ=255)                                    gCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGtt                                                                                                      <  2:511133/151‑1 (MQ=255)                                            ggACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACt                                                                                              <  2:610805/151‑1 (MQ=255)                                             gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                             <  2:624164/151‑1 (MQ=255)                                             gACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtg                                                                                             <  2:515930/151‑1 (MQ=255)                                                 gggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGTTGGATTCATTGCTACGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggt                                                                                           <  1:11815/149‑1 (MQ=255)                                                 gggTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACtggtgg                                                                                         <  2:517281/151‑1 (MQ=255)                                                                            taattaatTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTAc                                                              >  1:59527/1‑151 (MQ=255)                                                                               ttaattGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGt                                                           >  1:26311/1‑151 (MQ=255)                                                                                   ttGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTc                                                        >  2:382401/1‑150 (MQ=255)                                                                                    tGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCa                                                       >  2:199072/1‑150 (MQ=255)                                                                                       tCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTATTGTTTCAAAgg                                                   >  2:246223/1‑151 (MQ=255)                                                                                        cAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAgg                                                   <  2:589883/150‑1 (MQ=255)                                                                                         aaCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 <  2:458135/151‑1 (MQ=255)                                                                                         aaCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 <  1:551384/151‑1 (MQ=255)                                                                                          aCCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTa                                                 >  2:260593/1‑150 (MQ=255)                                                                                           ccTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAAt                                               <  2:71040/151‑1 (MQ=255)                                                                                                tCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGAt                                          >  1:489529/1‑151 (MQ=255)                                                                                                    ccGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGACGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGc                                      >  1:70994/1‑151 (MQ=255)                                                                                                     cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCt                                     >  1:51913/1‑151 (MQ=255)                                                                                                     cGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGCGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCt                                     >  1:377631/1‑151 (MQ=255)                                                                                                      gCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCtt                                    <  1:450649/151‑1 (MQ=255)                                                                                                       cTACACGGATGCTGGCGGATTCGGTACTGCGCGTCGCATTTTCCCTCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGGGGGGTGGGTATTTTTGGCGAGACTTCTACTGTTTCAAAGGCAATCTGTTCGGCTTc                                   >  1:445573/1‑151 (MQ=255)                                                                                                         acacGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCa                                  <  1:193262/150‑1 (MQ=255)                                                                                                           acGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATc                               >  1:6066/1‑151 (MQ=255)                                                                                                            cGGATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAAt                                >  1:550529/1‑149 (MQ=255)                                                                                                              gATGCTGGTGGATTCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATcac                             >  1:635644/1‑150 (MQ=255)                                                                                                                          ttCATTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTAcc                >  2:236174/1‑151 (MQ=255)                                                                                                                             aTTGCTGCGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCt             <  1:524983/151‑1 (MQ=255)                                                                                                                                 ctgcGCGTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCtgtg          >  2:241639/1‑150 (MQ=255)                                                                                                                                    cgcgTCGCTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGt      <  1:176861/151‑1 (MQ=255)                                                                                                                                       gtcgtTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCAt   >  2:334448/1‑151 (MQ=255)                                                                                                                                        tcgcTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATc  >  1:641047/1‑151 (MQ=255)                                                                                                                                           |                                                                                                                                                    CATGATTAACCGGGTCCAGTTCGCTGGAACGCTGGGCAAGCATGGACTGGGTGATAACCAGCTCGCCGACGAGGTTAATTAATTGATCAACCTTTTCTACCGCTACACGGATGCTGGTGGATTCATTGCTGCGCGTCGTTTTTTCCCGCTCCACGCGGCCGGTTGGCGCTTGTTCGGCTGCCAGTTTAAGCACTGGTGGGGTGGATATTTTTGGCGAGACTTCTACTGTTTCAAAGGTAATCTGATCGGCTTCAATCACAAAACAGAGTACCGCTGTGATGTCATC  >  NC_000913/1974424‑1974709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑