Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,977,549 T→C Y99C (TAC→TGC)  flhC ← flagellar class II regulon transcriptional activator, with FlhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,977,5490TC100.0% 79.0 / NA 22Y99C (TAC→TGC) flhCflagellar class II regulon transcriptional activator, with FlhD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/8);  total (14/8)

GTGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGG  >  NC_000913/1977404‑1977695
                                                                                                                                                 |                                                                                                                                                  
gTGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCt                                                                                                                                               >  1:630635/1‑151 (MQ=255)
  gggTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCttt                                                                                                                                             >  2:215894/1‑151 (MQ=255)
         aaaaTTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCAc                                                                                                                                       <  2:588263/150‑1 (MQ=255)
                 cgccgcAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGAc                                                                                                                              >  1:334852/1‑151 (MQ=255)
                  gccgcAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACg                                                                                                                             >  1:582832/1‑151 (MQ=255)
                     gcagcaGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGcc                                                                                                                           >  2:280522/1‑150 (MQ=255)
                                 gCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCg                                                                                                              <  1:90384/151‑1 (MQ=255)
                                                    ccACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGcc                                                                                           <  2:88193/151‑1 (MQ=255)
                                                              caaACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTAc                                                                                  >  1:490810/1‑150 (MQ=255)
                                                              caaACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTAc                                                                                  >  2:251676/1‑150 (MQ=255)
                                                                                      gggTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTg                                                         <  2:360386/151‑1 (MQ=255)
                                                                                           aaTGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTccc                                                    >  2:4930/1‑151 (MQ=255)
                                                                                                cagcagTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTc                                               >  1:566955/1‑151 (MQ=255)
                                                                                                     gTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATaa                                           >  1:190189/1‑150 (MQ=255)
                                                                                                       ggTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAAcc                                        >  2:191742/1‑151 (MQ=255)
                                                                                                             tcttctGCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTc                                    <  2:248423/149‑1 (MQ=255)
                                                                                                                   gCTTGTGGGCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGTTg                               <  2:279304/148‑1 (MQ=255)
                                                                                                                           gCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGt                                 >  2:17882/1‑138 (MQ=255)
                                                                                                                           gCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGt                                 <  1:17883/138‑1 (MQ=255)
                                                                                                                           gCACTGTTCAAGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGgcagc                    >  1:263582/1‑151 (MQ=255)
                                                                                                                                    aaGGTATAAACGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTc           <  1:191741/151‑1 (MQ=255)
                                                                                                                                             aCGGCAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTggg  >  2:150125/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GTGGGTAATAAAATTGCCGCCGCAGCAGTTGCAGCTGGAAAGTTGCAGTAATCCACTTTCAACAAACCGCACCAATGTCCAGGCACGGGTTAATGCCAGCAGTGGTCCTTCTTCTGCTTGTGGGCACTGTTCAAGGTATAAACGGTAGGCTTTGATCACCGCATCGACGCCATTACACAAACCGGTTTTCAGTAAAAACTGCCATGCATTACAGAACATCGAAGCATGAACGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGG  >  NC_000913/1977404‑1977695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: