Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,028,056	T→C	intergenic (‑39/+132)	yedQ ← / ← yodC	putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,028,056	0	T	C	100.0%	153.3 / NA	41	intergenic (‑39/+132)	yedQ/yodC	putative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/17);  total (24/17)

GAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTT  >  NC_000913/2027908‑2028198                                                                                                                                                     |                                                                                                                                               gAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACaa                                                                                                                                              <  2:194143/151‑1 (MQ=255)   aaaaaGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAAcc                                                                                                                                            <  1:107172/151‑1 (MQ=255)   aaaaaGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAAcc                                                                                                                                            <  2:130838/151‑1 (MQ=255)         aaaTACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCg                                                                                                                                       >  1:125294/2‑150 (MQ=255)           aTACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATa                                                                                                                                    <  1:407379/151‑1 (MQ=255)                 cGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTAc                                                                                                                              >  2:101795/1‑151 (MQ=255)                              aTTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCg                                                                                                                  >  1:113139/1‑150 (MQ=255)                                 aaCGACTTGCCCAGGCCCCAGCGGGCGGGCGA‑TTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATaa                                                                                                              <  2:652825/150‑1 (MQ=255)                                 aaCGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATa                                                                                                               >  2:253285/1‑150 (MQ=255)                                   cGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAAtt                                                                                                            >  1:320164/1‑151 (MQ=255)                                   cGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAAtt                                                                                                            <  2:214576/151‑1 (MQ=255)                                    gACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAAttt                                                                                                           <  1:608653/151‑1 (MQ=255)                                      cATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAAtttt                                                                                                          >  1:235257/1‑150 (MQ=255)                                       aTGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTtat                                                                                                        <  1:221826/151‑1 (MQ=255)                                        tGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTtat                                                                                                        >  2:224749/1‑150 (MQ=255)                                          aCCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGAt                                                                                                                >  1:191280/1‑140 (MQ=255)                                          aCCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGAt                                                                                                                <  2:191281/140‑1 (MQ=255)                                                  ccAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCAt                                                                                             >  1:322124/1‑151 (MQ=255)                                                     ggcggcGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGAt                                                                                          >  1:185803/1‑151 (MQ=255)                                                      gcggcgTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTTCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGAt                                                                                          >  2:360194/1‑150 (MQ=255)                                                      gcggcgTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGAt                                                                                          >  1:290171/1‑150 (MQ=255)                                                         gcgTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATa                                                                                         >  1:379449/1‑148 (MQ=255)                                                             gCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGAcgc                                                                                   <  2:2930/150‑1 (MQ=255)                                                                                     ttttCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCAtt                                                            >  2:30079/1‑149 (MQ=255)                                                                                      tttCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGc                                                         >  2:107030/1‑151 (MQ=255)                                                                                             ttttGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACAcc                                                    >  1:426679/1‑149 (MQ=255)                                                                                                  tctcGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCgg                                             >  1:282156/1‑151 (MQ=255)                                                                                                   ctcGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCgg                                             >  1:632671/1‑150 (MQ=255)                                                                                                   ctcGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTAATTGCAGGCATTAGCACACCAGGCCgg                                             >  1:516450/1‑150 (MQ=255)                                                                                                    tcGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCCAAACAGCGATAATTTTATACTGGCGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAt                                           >  1:633963/1‑151 (MQ=255)                                                                                                      gTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgt                                         >  2:615586/1‑151 (MQ=255)                                                                                                              cccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                  <  1:469406/150‑1 (MQ=255)                                                                                                                cTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                               >  1:207682/1‑151 (MQ=255)                                                                                                                     gacTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                          <  1:16954/151‑1 (MQ=255)                                                                                                                      acTCCCAACCTGCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                         <  1:87501/151‑1 (MQ=255)                                                                                                                                 gCTTTTTTATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                <  1:88148/149‑1 (MQ=255)                                                                                                                                    tttttATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc           >  1:2171/1‑151 (MQ=255)                                                                                                                                       ttATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac         >  1:442400/1‑150 (MQ=255)                                                                                                                                       ttATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac         <  1:3788/150‑1 (MQ=255)                                                                                                                                        tATGATTCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcaca        <  1:473307/150‑1 (MQ=255)                                                                                                                                              tCTGGCACAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtctt  <  1:152968/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               GAAAAAAGCAATACCACGATAAAGCAGAGATTAACGACATGACCAGGCCCCAGGCGGCGTGCGAGTTTTTTCAACCAGCTCTGGTTTTCCATTTTTGTCTCGTGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTT  >  NC_000913/2027908‑2028198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑