Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,036,629	A→G	A265A (GCA→GCG)	hchA →	glyoxalase III and Hsp31 molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,036,629	0	A	G	100.0%	134.2 / NA	39	A265A (GCA→GCG)	hchA	glyoxalase III and Hsp31 molecular chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/19);  total (20/19)

AAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTAT  >  NC_000913/2036481‑2036774                                                                                                                                                     |                                                                                                                                                  aaacGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGc                                                                                                                                                 <  2:489981/151‑1 (MQ=255)     gCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAAt                                                                                                                                             <  1:265227/151‑1 (MQ=255)          agaTTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGtt                                                                                                                                        <  1:112107/151‑1 (MQ=255)           gaTTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTg                                                                                                                                       <  2:64559/151‑1 (MQ=255)                gCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTa                                                                                                                                   >  2:427068/1‑150 (MQ=255)                 cTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTaa                                                                                                                                  >  2:249578/1‑150 (MQ=255)                    tatGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTg                                                                                                                              >  2:286673/1‑151 (MQ=255)                     atGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTgg                                                                                                                             >  2:669177/1‑151 (MQ=255)                             tCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGgcgc                                                                                                                       >  1:135581/1‑149 (MQ=255)                                    aCCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGc                                                                                                              >  1:255113/1‑151 (MQ=255)                                      cTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCt                                                                                                             >  1:26272/1‑150 (MQ=255)                                        ggTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTg                                                                                                            <  2:29650/149‑1 (MQ=255)                                              tCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCtt                                                                                                     <  2:607653/150‑1 (MQ=255)                                               cGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTAc                                                                                                   <  2:318102/151‑1 (MQ=255)                                                   gaagaaCTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCgg                                                                                               >  2:75669/1‑151 (MQ=255)                                                   gaagaaCTGAAGAAAATGGGCATGAATATCAGTAATGACGACATCGCCGGGCGAATACATAAGGACCGTAAACTTCTCACCGGCGACGGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTAcgcg                                                                                                <  1:18567/150‑1 (MQ=255)                                                       aaCTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTa                                                                                           <  2:401057/151‑1 (MQ=255)                                                                  aTGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTAc                                                                                >  1:308666/1‑151 (MQ=255)                                                                         tGAATATCATTAATGACGACATCCCCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGttt                                                                            >  1:529663/1‑148 (MQ=255)                                                                            atatCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGt                                                                      >  1:383858/1‑151 (MQ=255)                                                                                           acATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATaatcaat                                                       <  2:104780/151‑1 (MQ=255)                                                                                                   gggCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCcg                                               >  1:644565/1‑151 (MQ=255)                                                                                                       gAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCTGCCGCGtt                                           >  2:125910/1‑151 (MQ=255)                                                                                                          tACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGt                                        >  1:458749/1‑151 (MQ=255)                                                                                                           aCATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTa                                       <  1:112348/151‑1 (MQ=255)                                                                                                               aaGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCa                                   <  2:176212/151‑1 (MQ=255)                                                                                                                  gACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCacg                                 >  1:610458/1‑150 (MQ=255)                                                                                                                    ccGTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCacgac                               <  2:376095/150‑1 (MQ=255)                                                                                                                      gTAAACTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTg                            <  1:255051/151‑1 (MQ=255)                                                                                                                        aaaCTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTgg                           <  2:520767/150‑1 (MQ=255)                                                                                                                           cTTCTCACCGGCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGc                       >  2:500279/1‑151 (MQ=255)                                                                                                                                    ggCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTc               >  2:153435/1‑150 (MQ=255)                                                                                                                                    ggCGACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCa              <  2:39515/151‑1 (MQ=255)                                                                                                                                       gACAGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACgg           <  2:60459/151‑1 (MQ=255)                                                                                                                                          aGTCCTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGtt         >  1:395395/1‑150 (MQ=255)                                                                                                                                             ccTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAAt      <  2:631504/150‑1 (MQ=255)                                                                                                                                              cTTTTGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAAttt    >  2:334150/1‑151 (MQ=255)                                                                                                                                                tttGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTa   <  1:266311/150‑1 (MQ=255)                                                                                                                                                tttGCGGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTAt  >  2:202151/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AAACGCCAGAGATTGGCTATATGCCGGGTCATCTCACCTGGTACTTCGGCGAAGAACTGAAGAAAATGGGCATGAATATCATTAATGACGACATCACCGGGCGAGTACATAAGGACCGTAAACTTCTCACCGGCGACAGTCCTTTTGCAGCGAATGCGTTGGGTAAACTGGCGGCGCAGGAAATGCTGGCAGCTTACGCGGGTTAATCCCTGTTACGTTTGAATGTAAGCGCATAATCAATCGCGGCCGCGTTAGTACTCACGACGTGGCTGCGTTTTTTCACGGTTAATTTAT  >  NC_000913/2036481‑2036774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑