Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,691	T→C	intergenic (‑144/‑65)	dacD ← / → sbcB	D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b/exodeoxyribonuclease I; exonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,691	0	T	C	100.0%	154.9 / NA	43	intergenic (‑144/‑65)	dacD/sbcB	D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b/exodeoxyribonuclease I; exonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/20);  total (23/20)

CAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGC  >  NC_000913/2082545‑2082836                                                                                                                                                   |                                                                                                                                                  cAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCaaa                                                                                                                                                <  1:187089/150‑1 (MQ=255)   aCAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCt                                                                                                                                             >  1:457994/1‑151 (MQ=255)    cAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTg                                                                                                                                            <  2:215642/151‑1 (MQ=255)       cAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGAtt                                                                                                                                         <  1:457171/151‑1 (MQ=255)        aaaCCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGAtt                                                                                                                                         >  2:432975/1‑150 (MQ=255)           cccTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCa                                                                                                                                     <  2:47987/151‑1 (MQ=255)                aGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTg                                                                                                                                <  2:85672/151‑1 (MQ=255)                   aGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGata                                                                                                                             <  1:160264/151‑1 (MQ=255)                        cAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTg                                                                                                                         >  1:633862/1‑150 (MQ=255)                         aaaTAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGAt                                                                                                                       <  2:278911/151‑1 (MQ=255)                                    cTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGc                                                                                                               >  1:4748/1‑148 (MQ=255)                                               aaTACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATa                                                                                                 <  2:120979/151‑1 (MQ=255)                                               aaTACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATACTTGATACTCTGGCAGACAGCAGAAATa                                                                                                 <  2:253371/151‑1 (MQ=255)                                                aTACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATa                                                                                                 >  2:529633/1‑150 (MQ=255)                                                       tGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGAttt                                                                                         >  2:331090/1‑151 (MQ=255)                                                       tGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGAttt                                                                                         >  2:175995/1‑151 (MQ=255)                                                        gAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTa                                                                                        <  2:249318/151‑1 (MQ=255)                                                         aaCTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTa                                                                                        >  1:538873/1‑150 (MQ=255)                                                             ggctggAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAAcc                                                                                     <  1:4136/149‑1 (MQ=255)                                                                    aTAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAatgatg                                                                             >  1:290876/1‑150 (MQ=255)                                                                       aaGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGatgaatg                                                                         <  2:420892/151‑1 (MQ=255)                                                                             aaGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGt                                                                    >  2:531485/1‑150 (MQ=255)                                                                                   aTGTGACTGCCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGcaac                                                             <  2:4748/151‑1 (MQ=255)                                                                                   aTGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGcaa                                                              >  1:478953/1‑150 (MQ=255)                                                                                       gACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATc                                                         >  2:568151/1‑151 (MQ=255)                                                                                       gACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATc                                                         <  1:12984/151‑1 (MQ=255)                                                                                          tACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTAc                                                      >  2:337227/1‑151 (MQ=255)                                                                                             ccTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCtt                                                   >  2:191156/1‑151 (MQ=255)                                                                                               tctcATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCtttt                                                 >  1:264294/1‑151 (MQ=255)                                                                                                   aTTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGtt                                             <  2:669421/151‑1 (MQ=255)                                                                                                              aCATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGa                                       <  1:166139/146‑1 (MQ=255)                                                                                                              aCATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACg                                  <  2:77799/151‑1 (MQ=255)                                                                                                                aTGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGaa                                <  1:422595/151‑1 (MQ=255)                                                                                                                       gTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAAcc                             <  2:314915/147‑1 (MQ=255)                                                                                                                       gTTGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCttt                          <  1:55095/150‑1 (MQ=255)                                                                                                                         tGCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTgg                        >  1:570581/1‑150 (MQ=255)                                                                                                                          gCCACTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGgc                       >  2:291442/1‑150 (MQ=255)                                                                                                                             aCTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGgcacgc                   >  1:79832/1‑151 (MQ=255)                                                                                                                             aCTCGCTGCCAAATTGTGGCGCCAAAGCTGATTAGCACGGAGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGgcacgc                   >  2:360593/1‑151 (MQ=255)                                                                                                                                         aTTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGtt         >  2:475226/1‑149 (MQ=255)                                                                                                                                         aTTGTGGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGtt         >  1:230600/1‑149 (MQ=255)                                                                                                                                           tgtgGCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGtt         >  1:66684/1‑147 (MQ=255)                                                                                                                                              ggCGCCAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGc  >  2:219273/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTGGAATAAAGTGCAAGAAAATGTGACTACCCTCTCATTTTTATCTGACATGATCTGTTGCCACTCGCTGCCAAATTGTGGCGCTAAAGCTGATTAGCACGGTGATATTTGATACTCTGGCAGACAGCAGAAATAACGGATTTAACCTAATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGC  >  NC_000913/2082545‑2082836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑