Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,110,993	T→C	L9L (TTA→TTG)	rfbA ←	glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,110,993	0	T	C	100.0%	96.0 / NA	27	L9L (TTA→TTG)	rfbA	glucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

GAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGC  >  NC_000913/2110845‑2111140                                                                                                                                                     |                                                                                                                                                    gAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCaa                                                                                                                                                   <  1:272663/151‑1 (MQ=255)                       atCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATtttc                                                                                                                             <  2:296136/151‑1 (MQ=255)                                   cGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTc                                                                                                                 >  2:537966/1‑151 (MQ=255)                                    gCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTcc                                                                                                                >  1:45685/1‑151 (MQ=255)                                         cATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCtttt                                                                                                            >  1:502258/1‑150 (MQ=255)                                         cATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTa                                                                                                           <  1:282097/151‑1 (MQ=255)                                               tgtAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCa                                                                                                      <  1:476931/150‑1 (MQ=255)                                                     gagCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCt                                                                                               >  2:516537/1‑151 (MQ=255)                                                         gggTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTccac                                                                                           <  2:245813/151‑1 (MQ=255)                                                                aGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCAc                                                                                     <  2:363354/150‑1 (MQ=255)                                                                 gATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACg                                                                                    >  2:179920/1‑150 (MQ=255)                                                                       cGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGa                                                                             <  1:551363/151‑1 (MQ=255)                                                                             aTCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCaa                                                                        >  1:445884/1‑150 (MQ=255)                                                                                  aaaTAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTa                                                                  <  2:237978/151‑1 (MQ=255)                                                                                   aaTAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTAt                                                                 >  2:273128/1‑151 (MQ=255)                                                                                    aTAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTAtt                                                                >  1:648129/1‑151 (MQ=255)                                                                                      aGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTa                                                               >  1:597412/1‑150 (MQ=255)                                                                                          aaTAGCTGTTTACTGACAGCCATATTCACAGGATAAAGACGTTTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAAt                                                            <  1:7549/149‑1 (MQ=255)                                                                                          aaTAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTg                                                          <  1:548975/151‑1 (MQ=255)                                                                                                  tttACTGACAGCCATAGTCACAGTATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGt                                                   <  1:258891/150‑1 (MQ=255)                                                                                                   ttACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTc                                                  <  1:506292/150‑1 (MQ=255)                                                                                                           atccATAGTCACAGGATAAAGACGTGTACCCGAACCACCCGCCCAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAaataat                                         >  2:504989/3‑151 (MQ=255)                                                                                                                     acaGGATAAAGACGTGTACCAGAACCACCCGCCAAAATAATGCCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGc                                <  1:297134/150‑1 (MQ=255)                                                                                                                       aGGATAAATGCTTGTACCAGCTCAACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCTCCATCACGAACAAGATGCAAAACCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAg                                 <  1:285028/147‑1 (MQ=255)                                                                                                                                    gtACCAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCa                 >  1:13867/1‑150 (MQ=255)                                                                                                                                       ccAGAACCACCCGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATCGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCt             <  1:537345/151‑1 (MQ=255)                                                                                                                                                  cGCCAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGc  >  2:517518/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GAGGTGTACTGATAATCAAAATATCGCGAATACCCGCCAACATCAGTGTAGAGAGCGGGTAATAGATCATCGGTTTATCATAAATAGGTAATAGCTGTTTACTGACAGCCATAGTCACAGGATAAAGACGTGTACCAGAACCACCCGCTAAAATAATACCTTTACGCATTTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGC  >  NC_000913/2110845‑2111140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑