Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,605	T→C	G320G (GGA→GGG)	fbaB ←	fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,605	0	T	C	100.0%	131.8 / NA	37	G320G (GGA→GGG)	fbaB	fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

CCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTG  >  NC_000913/2177470‑2177742                                                                                                                                        |                                                                                                                                          ccTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGcccc                                                                                                                            <  2:113834/151‑1 (MQ=255)         gTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGcc                                                                                                                    >  2:540343/1‑151 (MQ=255)          tCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCt                                                                                                                   >  1:404399/1‑151 (MQ=255)           ccTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTg                                                                                                                  <  2:642334/151‑1 (MQ=255)           ccTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTg                                                                                                                  <  2:128390/151‑1 (MQ=255)              aCGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTgcgc                                                                                                               >  2:292222/1‑151 (MQ=255)                   gtggtgTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTttg                                                                                                          <  2:2152/151‑1 (MQ=255)                      gtgtTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTttgttg                                                                                                       <  1:632356/151‑1 (MQ=255)                       tgttgtTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGcgcg                                                                                                              <  2:42838/143‑1 (MQ=255)                       tgttgtTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGcgcg                                                                                                              >  1:42839/1‑143 (MQ=255)                              cGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGc                                                                                               <  2:642118/151‑1 (MQ=255)                                          aTCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGc                                                                                   >  1:582039/1‑151 (MQ=255)                                                  aTAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGa                                                                           <  1:15729/151‑1 (MQ=255)                                                  aTAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGa                                                                           <  2:52310/151‑1 (MQ=255)                                                   tAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTg                                                                            >  1:634548/1‑149 (MQ=255)                                                    aGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAgg                                                                         <  1:171289/151‑1 (MQ=255)                                                        aTTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTc                                                                       >  1:462096/1‑149 (MQ=255)                                                         ttttGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGt                                                                    >  1:80665/1‑151 (MQ=255)                                                         ttttGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGt                                                                    >  1:109932/1‑151 (MQ=255)                                                              cTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAc                                                               >  1:238560/1‑151 (MQ=255)                                                                aTCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAcc                                                              <  2:78669/150‑1 (MQ=255)                                                                    agaTAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAccgcccg                                                         <  1:513132/151‑1 (MQ=255)                                                                       tAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCAccgcccgc                                                        >  2:201246/1‑149 (MQ=255)                                                                          aCGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCAc                                                   >  2:150922/1‑151 (MQ=255)                                                                             tCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCAccgc                                                >  2:361612/1‑151 (MQ=255)                                                                              ccTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCAgcgcc                                               <  2:73278/151‑1 (MQ=255)                                                                                   aCGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAg                                           >  2:215824/1‑150 (MQ=255)                                                                                   aCGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGt                                          >  1:565024/1‑151 (MQ=255)                                                                                    cGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGtt                                         >  1:335589/1‑151 (MQ=255)                                                                                       cGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTAt                                      <  2:179577/151‑1 (MQ=255)                                                                                          tAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCa                                    <  2:326018/150‑1 (MQ=255)                                                                                               aGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAg                              <  2:246205/151‑1 (MQ=255)                                                                                                      cGCCGTCAGCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGAcc                       <  1:312736/151‑1 (MQ=255)                                                                                                              gCCATCGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGc               >  1:626436/1‑151 (MQ=255)                                                                                                                   cGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGtt           >  1:436111/1‑150 (MQ=255)                                                                                                                   cGATTTCTTGAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGtt           >  2:631674/1‑150 (MQ=255)                                                                                                                            gAACGCTTTACGCCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTg  >  2:103628/1‑150 (MQ=255)                                                                                                                                        |                                                                                                                                          CCTTATCCGTCCTACGGGGTGGTGTTGTTCGTATAAGGCGTATCAGGCGATAGTAATTTTGCTATCGAGATAAACGTCCTGCACGGCGTTAATCAGTTTCACGCCGTCAGCCATCGATTTCTTGAACGCTTTACGTCCAAGAATCAGCCCCATTCCGCCTGCGCGTTTGTTGATAACCGCAGTACGCACTGCATCGCTGAGGTCAGTTTCACCGCCCGCAGCACCGCCGGAGTTTATCAACCCAGCCCGACCCATATAGCAGTTAGCTAACTG  >  NC_000913/2177470‑2177742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑