Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,252,698	A→G	V333V (GTA→GTG)	yeiI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,698	0	A	G	100.0%	112.5 / NA	38	V333V (GTA→GTG)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

CGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGC  >  NC_000913/2252554‑2252841                                                                                                                                                 |                                                                                                                                                  cGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTgc                                                                                                                                             <  2:539145/151‑1 (MQ=255)      aGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAACTTTTTTTTAGGGGAAAGTTTTCGCGACAGCGCCCGGTTTTTGGGGGGCTGCGC‑gg                                                                                                                                        >  2:46255/1‑150 (MQ=255)         aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAAt                                                                                                                                    <  2:17295/151‑1 (MQ=255)            gagaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTc                                                                                                                                 <  2:532872/151‑1 (MQ=255)                     ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATTTAATTTTTTTTTAGGGTAAAGTTTTCTCGGCAGCGCCCCGTTTTCGGTGGGCCGCGCGGGCAATTT‑GCGCGcc                                                                                                                          >  1:382813/1‑149 (MQ=255)                      tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTGTATAACTTTTTTTTAGGGTACAGTTTTCGCGGCAACGCCCCCTTTGCGGGGGGCTGCGC‑GGCCATTTTGCGCGCCCGc                                                                                                                       >  1:349157/1‑151 (MQ=255)                       ttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGc                                                                                                                       <  2:361507/150‑1 (MQ=255)                        tGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTCTTTAAGGATAAAGTTTTCGCCACAGCGCCCGCTTTTCGGGGGGCTGCGC‑GGCCATTTCTCGCGCCCGC‑gg                                                                                                                    >  2:156401/1‑151 (MQ=255)                                 cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTaaa                                                                                                           <  2:294263/151‑1 (MQ=255)                                 cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATAGCTTTTTTTGAGGGTACAGTTTTCGCGACAACGCCCGCTTTTCGGTGGGCTGCGC‑GGCAATTTTTCGCGCCAAC‑GGCCGCGTaa                                                                                                            >  2:378744/1‑150 (MQ=255)                                      gcgcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAaacaac                                                                                                       <  1:53646/150‑1 (MQ=255)                                        gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGCTTTTTTTTAGGAAAAAATTTTCGCGGCAGCGCCCGGTTTTTGGTGGGCTGCGC‑GGGGAATTTTCGCGCCCGCGGGCGCCTTAAAAAacc                                                                                                      >  1:153104/1‑150 (MQ=255)                                           tACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTTTTTAAGGGTACAGATTTCCCGACAGCGCCCGCTTTGCGGTGGGCCGCGC‑GGCAATTTTGCGCGCCCGC‑GGCCGCCTAAACAaacccacc                                                                                                 >  1:523803/1‑151 (MQ=255)                                            aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAaccctaccc                                                                                                <  2:157021/151‑1 (MQ=255)                                                  gTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTg                                                                                          <  2:242800/151‑1 (MQ=255)                                                          ttttGGTGCTGACGATGGTTTTATGGCGGGGCCGGTGTATAACTTTTTTTTAGGGGAAAGGTTTTGCGGCCGCGCCCCCTTTTTTGTGGGCCGGGC‑GGCCATTTTTCCCGCCCCCGGGCGGCTTAAAAAACCC‑CCCCCGTCTGCCGATaa                                                                                   >  1:506864/1‑150 (MQ=255)                                                                             tttATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCa                                                                <  2:307508/150‑1 (MQ=255)                                                                                aTGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg                                                            <  2:311651/151‑1 (MQ=255)                                                                                 tGGCGGGCCTGGAATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCATGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg                                                            >  2:545645/1‑150 (MQ=255)                                                                                    cGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtgt                                                         >  1:27478/1‑150 (MQ=255)                                                                                    cGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa                                                        <  1:19506/151‑1 (MQ=255)                                                                                     gggCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTaa                                                       <  2:382817/151‑1 (MQ=255)                                                                                     gggCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa                                                        >  2:614402/1‑150 (MQ=255)                                                                                              atataGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg                                               >  2:60091/1‑150 (MQ=255)                                                                                              atataGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg                                               <  1:149300/150‑1 (MQ=255)                                                                                               tataGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGcc                                             >  1:426453/1‑151 (MQ=255)                                                                                                   gCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAt                                         <  2:349482/151‑1 (MQ=255)                                                                                                                  aCAGTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAAcgc                           <  2:153107/150‑1 (MQ=255)                                                                                                                     gTTTCCGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCt                       >  2:301272/1‑151 (MQ=255)                                                                                                                         ccGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTAt                    <  1:196497/150‑1 (MQ=255)                                                                                                                         ccGCGACAGCGCCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATc                   <  1:244171/151‑1 (MQ=255)                                                                                                                                 gcgcCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTAc           <  2:628106/151‑1 (MQ=255)                                                                                                                                   gcCCGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACa          >  2:347353/1‑150 (MQ=255)                                                                                                                                      cGTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATc      >  1:614630/1‑151 (MQ=255)                                                                                                                                       gTTTTGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCa     <  1:617915/151‑1 (MQ=255)                                                                                                                                         tttGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAg    <  2:364979/150‑1 (MQ=255)                                                                                                                                         tttGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAg    <  2:20770/150‑1 (MQ=255)                                                                                                                                          ttGCGGTGGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGc  >  2:593376/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGC‑GGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGCGACGCTTAACGCGTCTTATCCGGCCTACAAATCAGGC  >  NC_000913/2252554‑2252841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑