Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,214	(A)6→5	coding (590/1680 nt)	dauA ←	C4‑dicarboxylic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,209	0	A	.	100.0%	389.8 / NA	81	coding (595/1680 nt)	dauA	C4‑dicarboxylic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (43/38);  total (43/38)

ACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCA  >  NC_000913/1260063‑1260355                                                                                                                                                   |                                                                                                                                                   aCCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCaaaaa                                                                                                                                                <  2:78892/151‑1 (MQ=255) aCCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCaaaaa                                                                                                                                                <  2:267985/151‑1 (MQ=255) aCCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCaaaa                                                                                                                                                 <  2:206473/150‑1 (MQ=255)    cTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCaaaaaca                                                                                                                                              >  2:1625365/1‑148 (MQ=255)        gaACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAAt                                                                                                                                        <  2:1569803/151‑1 (MQ=255)           ccATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATAcc                                                                                                                                     <  3:344366/151‑1 (MQ=255)             aTCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTa                                                                                                                                   <  2:467340/151‑1 (MQ=255)               cGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAg                                                                                                                                  <  3:316297/150‑1 (MQ=255)                   cAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGt                                                                                                                               >  2:1427899/1‑149 (MQ=255)                        cGTAGTGGAATTGCGAACCGATGGAAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAA‑GAATGCCCAAACGCCGCC‑CAAAACAAAAAAACCTAGGCTCACaa                                                                                                                          >  2:1555694/1‑148 (MQ=255)                           aGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGcc                                                                                                                      <  4:1467809/150‑1 (MQ=255)                              ggAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAAt                                                                                                                   <  4:702985/150‑1 (MQ=255)                                aaTTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGgc                                                                                                                >  4:673343/1‑151 (MQ=255)                                 aTTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGgc                                                                                                                >  2:1510911/1‑150 (MQ=255)                                  ttGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGgc                                                                                                                >  4:803789/1‑149 (MQ=255)                                         ccGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCAcc                                                                                                       >  2:1437568/1‑151 (MQ=255)                                           gATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCAcac                                                                                                      >  2:387702/1‑148 (MQ=255)                                              ggTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACAt                                                                                                  >  2:1185742/1‑151 (MQ=255)                                               gTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACAtt                                                                                                 <  1:86602/151‑1 (MQ=255)                                                   cAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCTCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATg                                                                                             <  1:104394/151‑1 (MQ=255)                                                   cAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATg                                                                                             <  4:1103474/151‑1 (MQ=255)                                                      cATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTc                                                                                          >  4:1590629/1‑151 (MQ=255)                                                       aTGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGt                                                                                           >  4:503571/1‑149 (MQ=255)                                                         gTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCgg                                                                                        <  2:419600/150‑1 (MQ=255)                                                         gTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGc                                                                                       >  2:1385412/1‑151 (MQ=255)                                                             gccgAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAgcg                                                                                   >  2:580941/1‑151 (MQ=255)                                                              ccgAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAgcgc                                                                                  >  1:100577/1‑151 (MQ=255)                                                              ccgAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAgcgc                                                                                  >  2:1092569/1‑151 (MQ=255)                                                                   cAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCata                                                                              >  3:269025/1‑150 (MQ=255)                                                                   cAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCata                                                                              >  2:1636442/1‑150 (MQ=255)                                                                     agTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCataaat                                                                           >  2:1105157/2‑151 (MQ=255)                                                                      gTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCataaata                                                                          <  2:579023/151‑1 (MQ=255)                                                                        tAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCataaataa                                                                         <  3:192615/150‑1 (MQ=255)                                                                         aaCAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATg                                                                       >  2:1071740/1‑151 (MQ=255)                                                                            aaTCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATgcgc                                                                    >  2:1360571/1‑151 (MQ=255)                                                                             aTCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGcc                                                                   <  3:283055/151‑1 (MQ=255)                                                                             aTCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGcc                                                                   >  4:1534312/1‑151 (MQ=255)                                                                              tCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCg                                                                  >  2:238161/1‑151 (MQ=255)                                                                                  cATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACtt                                                              <  2:733800/151‑1 (MQ=255)                                                                                   aTCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACttt                                                             >  2:762827/1‑151 (MQ=255)                                                                                   aTCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACttt                                                             <  2:263340/151‑1 (MQ=255)                                                                                     cACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACtttt                                                            <  2:647057/150‑1 (MQ=255)                                                                                      aCCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTg                                                          >  2:79921/1‑151 (MQ=255)                                                                                       ccGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGt                                                         >  1:283999/1‑151 (MQ=255)                                                                                        cgcgCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGt                                                         >  1:182599/1‑150 (MQ=255)                                                                                        cgcgCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTa                                                        <  1:235374/151‑1 (MQ=255)                                                                                         gcgcAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGt                                                         >  4:152108/1‑149 (MQ=255)                                                                                             aaCCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATa                                                    >  3:175857/1‑150 (MQ=255)                                                                                              aCCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAAt                                                  >  2:8423/1‑151 (MQ=255)                                                                                                cagccagcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGt                                                <  4:1215582/151‑1 (MQ=255)                                                                                                   ccagcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTcc                                             <  2:587075/151‑1 (MQ=255)                                                                                                   ccagcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAAAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTcc                                             <  4:486143/151‑1 (MQ=255)                                                                                                    cagcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCg                                            >  4:224482/1‑151 (MQ=255)                                                                                                     agcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCgg                                           >  4:134891/1‑151 (MQ=255)                                                                                                     agcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCgg                                           <  4:1310499/151‑1 (MQ=255)                                                                                                     agcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCgg                                           <  4:1187237/151‑1 (MQ=255)                                                                                                        aaGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGAc                                        <  3:165939/151‑1 (MQ=255)                                                                                                        aaGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGAc                                        <  4:441448/151‑1 (MQ=255)                                                                                                        aaGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGAc                                        >  4:251070/1‑151 (MQ=255)                                                                                                          ggCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACAt                                      <  1:465065/151‑1 (MQ=255)                                                                                                              ggAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGc                                  <  4:1429685/151‑1 (MQ=255)                                                                                                              ggAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGc                                  >  3:378280/1‑151 (MQ=255)                                                                                                               gAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGcc                                 >  4:530312/1‑151 (MQ=255)                                                                                                               gAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGcc                                 <  2:672418/151‑1 (MQ=255)                                                                                                                 aGGTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCAt                               <  4:771102/151‑1 (MQ=255)                                                                                                                  ggTGACCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCAtt                              >  2:1268802/1‑151 (MQ=255)                                                                                                                      aCCGGGTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCa                          <  2:271126/151‑1 (MQ=255)                                                                                                                         gggTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGAc                       <  4:121926/151‑1 (MQ=255)                                                                                                                         gggTAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGAc                       >  2:139590/1‑151 (MQ=255)                                                                                                                            tAAACGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCg                     >  3:287749/1‑150 (MQ=255)                                                                                                                             aaaCGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCga                    <  2:569761/150‑1 (MQ=255)                                                                                                                             aaaCGAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCg                     >  4:7370/1‑149 (MQ=255)                                                                                                                                 gAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGaaaa               <  2:164345/151‑1 (MQ=255)                                                                                                                                 gAATGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGaaa                >  4:1661061/1‑150 (MQ=255)                                                                                                                                   aTGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATc             >  4:1005380/1‑151 (MQ=255)                                                                                                                                    tGCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCt            >  2:1089738/1‑151 (MQ=255)                                                                                                                                     gCCCAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCtt           <  3:389560/151‑1 (MQ=255)                                                                                                                                      cccAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCttt          <  2:207522/151‑1 (MQ=255)                                                                                                                                        cAGACGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTa         >  2:176514/1‑150 (MQ=255)                                                                                                                                           aCGCGGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCt     >  2:643329/1‑151 (MQ=255)                                                                                                                                            cgcgGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTg    <  4:653368/151‑1 (MQ=255)                                                                                                                                            cgcgGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTg    <  2:1450513/151‑1 (MQ=255)                                                                                                                                             gcgGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGc   >  2:1169029/1‑151 (MQ=255)                                                                                                                                             gcgGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGc   <  2:1440455/151‑1 (MQ=255)                                                                                                                                              cgGCC‑AAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCa  <  4:1580205/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   ACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCA  >  NC_000913/1260063‑1260355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑