Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,0550AC43.6% 199.4 / 22.5 110V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (51/11);  new base C (0/48);  total (51/59)
Fisher's exact test for biased strand distribution p-value = 6.98e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTT  >  NC_000913/3260908‑3261204
                                                                                                                                                   |                                                                                                                                                     
tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAccc                                                                                                                                                    >  4:1344321/1‑151 (MQ=255)
 aGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                                                                                   <  3:335832/151‑1 (MQ=255)
 aGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                                                                                   <  4:304654/151‑1 (MQ=255)
  gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                                                                                  >  3:220777/1‑151 (MQ=255)
   tGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgc                                                                                                                                                 >  1:242322/1‑151 (MQ=255)
   tGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccg                                                                                                                                                  <  1:20492/150‑1 (MQ=255)
            ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATACAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                                                                                         >  3:16701/1‑150 (MQ=255)
                gcgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                     >  2:1394885/1‑150 (MQ=255)
                 cgcgcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGt                                                                                                                                     >  3:243292/1‑149 (MQ=255)
                  gcgcTGATGTCCTGCCCCCCCAGCCAGCCCAGGAAGTGATGGAGGCTGTCCATCACTTTGTCGTAACCCAGCACGTCATCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTag                                                                                                                                   <  4:29386/150‑1 (MQ=255)
                    gcTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                                >  2:559844/1‑151 (MQ=255)
                     cTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                                >  4:117753/1‑150 (MQ=255)
                      tgttGTACTGCCCCGCCACCCAGTCCATGAAGTGATCGGGGCTGTCCATCATTTTGTCGTAATCCAGCACGTCGTCCACCAGCGGTGCTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTaga                                                                                                                                  <  3:320554/144‑1 (MQ=255)
                           tACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGttt                                                                                                                         >  4:1225103/1‑151 (MQ=255)
                             ctacACCGCCACCCGGTCCATGGAGTGACCGACGCTTCCCGTCTTTTTCTGAAACTCCATCACCCCTTCCTCAATCGGTTTTTTTTGCGGCCCTCCCGCTATCTTCAGCTTCTCTTCCACCCCGCCGTTAATTGCGTAGAGCAGCGtttt                                                                                                                        <  4:1390180/147‑1 (MQ=255)
                              tGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCg                                                                                                                      >  1:401827/1‑151 (MQ=255)
                                ccccGCCACCCAGTCCATAAAGTGATCGAGGCTGTCCATCATTTTGTCGTAACCCAGCCCGTCGCCCACCAGCGGTGCTGTTTTCGCCCCGCCCTAAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc                                                                                                                     <  4:549424/148‑1 (MQ=255)
                                cACCGCCAGCCAGTCCATGAAGTGATCGGGGCTGCCCATCCCTTTGTCGTAACCCAGCACGTCGTCCATCAGCGTTGTTTTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGttt                                                                                                                         <  2:1616258/146‑1 (MQ=255)
                                   cGCCAGCCGGTCCATGAAGTGATCGAGGCTGTCCATCCTTTTGTCGTAATCCAGCCCGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGACCTGATTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAgg                                                                                                                 <  4:62383/151‑1 (MQ=255)
                                    gccagccaGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                                >  4:133273/1‑151 (MQ=255)
                                    gccagccaGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGt                                                                                                                >  4:1026747/1‑151 (MQ=255)
                                     ccgccccgcccATGAATTGACCGGGCCTGTCCATCCCTTTGCCGTACCCCAGCCCGTCGCCCACCGCCGGTGTTTTTTTCGCCCCGACCTGAATCTCCAGCTTCTCCTCCCCCCCGCCGTTAATTTCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                               <  2:63347/142‑1 (MQ=255)
                                       agccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                               >  2:124699/1‑149 (MQ=255)
                                         ccagTCCATGAAGTGATCGGGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGTTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAg                                                                                                             <  4:615340/149‑1 (MQ=255)
                                         ccagTCCATGAAGTGATCGAGGCTGTCCATCCTTTTGTCGTAACCCAGCACGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                              <  4:546661/148‑1 (MQ=255)
                                         ccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAACCCAGCACGTCGTCCACCAGCGTTGCTTTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                              <  3:95621/148‑1 (MQ=255)
                                           agTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg                                                                                                         >  4:1158634/1‑151 (MQ=255)
                                            gTCCAAGAAGTGATCCAGTCTGTCAATCTTTTGCTCGTAACCCACCGCATCGCCCATCAGGGGTTCTTTTTTCGCCCCGACCTAACTCTTCTGTTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                              <  2:544800/145‑1 (MQ=255)
                                            gTCAATGAAGTGATCGAGGCTGTCCATCATTTTGTCGTAACCCAGCACGTCGTCCATCAGCGGTGCTTTTTTCGCCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGt                                                                                                        <  2:1352037/151‑1 (MQ=255)
                                              ccATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGc                                                                                                      >  4:1616765/1‑151 (MQ=255)
                                              ccAGGAGGTGATCGAGCATTTCATTCACTTTGTCTTAACCCAGCCTCTTGTCAACCGGCGGTTCTTTTTCCGGCCCACCCAGAATCTCCCGCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcgc                                                                                                          <  4:144097/147‑1 (MQ=255)
                                                  gAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcc                                                                                                   >  4:1614073/1‑150 (MQ=255)
                                                   aaGTGATGGAGGCTTTCCATCCCTTTGTCGTACTCCAGCCCGTCTTCCATCAGGGGTGCTGTTTTCGCCCCCACTTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAc                                                                                                    <  1:342097/148‑1 (MQ=255)
                                                    aGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                                 >  1:61037/1‑150 (MQ=255)
                                                     gTGATCGGGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaa                                                                                                <  3:72355/150‑1 (MQ=255)
                                                       gATCGAGGCTGTCCACCACTTTGTCGAAACCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGATTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGaa                                                                                             <  4:387115/151‑1 (MQ=255)
                                                          cGAGGCTGTCCATCACTTTGTCGTAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg                                                                                          <  4:885260/151‑1 (MQ=255)
                                                           gAGGCTGCCCATCACTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                                                                                         <  4:1283275/151‑1 (MQ=255)
                                                              gCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAtt                                                                                      >  2:436468/1‑151 (MQ=255)
                                                                   ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAttt                                                                                     <  3:401844/147‑1 (MQ=255)
                                                                   ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                 >  1:440441/1‑151 (MQ=255)
                                                                   ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                 >  4:725005/1‑151 (MQ=255)
                                                                   ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                 >  2:1291915/1‑151 (MQ=255)
                                                                   ccATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCATCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                 >  1:90290/1‑151 (MQ=255)
                                                                    cATCGCTTTGTCGTAATCCGCCCCGTCGCCCATCGGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCACCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTa                                                                                <  2:1544851/151‑1 (MQ=255)
                                                                    cATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                 >  3:316566/1‑150 (MQ=255)
                                                                    cATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCtt                                                                                 >  4:364672/1‑150 (MQ=255)
                                                                     aTCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc                                                                               >  2:1128733/1‑151 (MQ=255)
                                                                      tCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGGGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                                                                              >  2:266478/1‑151 (MQ=255)
                                                                       cACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                                                                              >  1:278499/1‑150 (MQ=255)
                                                                        aCTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                                                                             >  2:945657/1‑150 (MQ=255)
                                                                          tttGTCTTAATCCAGCACGCCGCCCATCGGGGGTGTTGTTTTCGCCCCGACTTGAATTTCCACCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc                                                                               <  2:1223722/146‑1 (MQ=255)
                                                                          tttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                          >  3:115065/1‑151 (MQ=255)
                                                                          tttGTCGTAACCCACCACGCCGTCCATCAGCGGTGCTTTTTTCGTCCCGACCTTATTCTTCAGCTTCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAAt                                                                           <  4:1130170/150‑1 (MQ=255)
                                                                           ttGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                          >  4:1316906/1‑150 (MQ=255)
                                                                           ttGTCGTAATCCACCACGTCGTCCATCAGCGGTTCTGTTTGCGGCCCGCCCTGAATCTCCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGc                                                                                   <  2:661798/141‑1 (MQ=255)
                                                                           ttGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGTTTTTTTCGCCCCGCCCTGATTCTTCAGCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATc                                                                          <  4:1435977/150‑1 (MQ=255)
                                                                            tGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCAc                                                                        >  2:117973/1‑151 (MQ=255)
                                                                            tGTCGTAATCCAGCACGCCGTCCACCAGGGGTCCTGTTTTCGGCCCGCCGTGATTCTTCGGCTTCCCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCa                                                                         <  4:1055408/150‑1 (MQ=255)
                                                                                 tAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTg                                                                   >  3:358908/1‑151 (MQ=255)
                                                                                  aaTCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTgg                                                                  >  1:57246/1‑151 (MQ=255)
                                                                                     ccAGCACGTCGTCCATCGGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGc                                                                <  2:418090/150‑1 (MQ=255)
                                                                                     ccAGCACGTCGTCCATCAGCGGTGCTGTTTTCGCCCCGCCCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTggg                                                                 <  4:1468449/149‑1 (MQ=255)
                                                                                       agaccgactcccATCAGGGTTGTTTTTTTCGGCCCGACTTGAATCTTCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAt                                                                     <  2:1602334/133‑1 (MQ=255)
                                                                                         cccgtcgcCCATCAGCGGTGCTGTTTTCGCCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGAc                                                            <  4:841581/148‑1 (MQ=255)
                                                                                           cgtcgtCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGAc                                                            <  4:27562/148‑1 (MQ=255)
                                                                                           cgtcgtCCATCAGCGGTGCTGTTTGCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgca                                                         <  3:200915/151‑1 (MQ=255)
                                                                                               gtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc                                                       >  1:117552/1‑149 (MQ=255)
                                                                                                 ccATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAgg                                                    <  4:62161/150‑1 (MQ=255)
                                                                                                       gggttGCTGTTTTCTGCGTGTGTTGGATCTCCAGCTTCTCTCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATc                                               <  4:950803/145‑1 (MQ=255)
                                                                                                         ggggCTGTTTTCGGCCCGCCTTGAATCTAAACTTCCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg                                            <  4:1069194/147‑1 (MQ=255)
                                                                                                         ggTGTTGTTTTCGGCCCGACCTGAATCTTCAGCTCCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg                                            <  4:1517193/150‑1 (MQ=255)
                                                                                                         ggTGCTGTTTTCGGCCCGACCTAAATCTTCACCTTCTCTTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATc                                               <  4:1396633/147‑1 (MQ=255)
                                                                                                         ggTGCTGTTTCCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg                                            <  4:88613/150‑1 (MQ=255)
                                                                                                           tgttGTTTTCGGCCCGACCTGAATCTTCAGTTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATc                                               <  1:51667/142‑1 (MQ=255)
                                                                                                              tGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtc                                       <  4:3368/150‑1 (MQ=255)
                                                                                                                ttttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                    >  1:319879/1‑151 (MQ=255)
                                                                                                                tttcCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcg                                      <  2:777054/149‑1 (MQ=255)
                                                                                                                tttcCGCCCCGCCCTAAACCTTCATCTTCCCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgt                                     <  2:838445/150‑1 (MQ=255)
                                                                                                                  ttCGCCCCGCCCTGATTCTTCAGCTCCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtc                                    <  2:1140246/149‑1 (MQ=255)
                                                                                                                     ggCCCGCCCTGAATTTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                 <  2:1470836/149‑1 (MQ=255)
                                                                                                                     ggCCCGCCCGGAATCTCCAGCTTCCCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                 <  2:1095789/149‑1 (MQ=255)
                                                                                                                         cGACCAGAATCTTCAGCTTCCCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCGCCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGaa                           <  2:1342640/151‑1 (MQ=255)
                                                                                                                          gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGa                            >  2:1462550/1‑149 (MQ=255)
                                                                                                                          gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGa                            >  4:20692/1‑149 (MQ=255)
                                                                                                                            ccgaaaTCTTCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                        <  1:358891/147‑1 (MQ=255)
                                                                                                                            ccTGATTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAg                          <  2:1157166/149‑1 (MQ=255)
                                                                                                                             cTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                        >  4:1627807/1‑150 (MQ=255)
                                                                                                                              tGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                        >  4:562711/1‑149 (MQ=255)
                                                                                                                              tGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc                        >  1:88062/1‑149 (MQ=255)
                                                                                                                                aaTCTTCATCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTa                    <  2:764022/151‑1 (MQ=255)
                                                                                                                                 aTCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTAc                   >  4:725561/1‑151 (MQ=255)
                                                                                                                                  tCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg                  >  2:38875/1‑151 (MQ=255)
                                                                                                                                     tCACCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCa                <  2:1153182/150‑1 (MQ=255)
                                                                                                                                       aGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc              >  4:751080/1‑150 (MQ=255)
                                                                                                                                       aGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc              >  3:302868/1‑150 (MQ=255)
                                                                                                                                       aGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCa             <  2:724029/151‑1 (MQ=255)
                                                                                                                                          ttCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAg            <  1:250851/149‑1 (MQ=255)
                                                                                                                                               gTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTc      >  4:1288700/1‑150 (MQ=255)
                                                                                                                                                 ccACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtt   >  4:4587/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTT  >  NC_000913/3260908‑3261204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑