Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC42.1% 216.2 / 31.2 114V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (62/4);  new base C (0/48);  total (62/52)
Fisher's exact test for biased strand distribution p-value = 7.19e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427
                                                                                                                                                        |                                                                                                                                                      
   aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTAcccc                                                                                                                                                    >  2:178731/1‑151 (MQ=255)
   aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTAcccc                                                                                                                                                    >  4:598385/1‑151 (MQ=255)
    cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTAccccc                                                                                                                                                   >  2:277683/1‑151 (MQ=255)
     gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCg                                                                                                                                                  >  3:404663/1‑151 (MQ=255)
      cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGc                                                                                                                                                 <  2:1535351/151‑1 (MQ=255)
       aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGcc                                                                                                                                                >  2:1411821/1‑151 (MQ=255)
          gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCat                                                                                                                                              >  2:1247216/1‑150 (MQ=255)
             tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTc                                                                                                                                          >  2:354979/1‑151 (MQ=255)
                    tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTc                                                                                                                                    >  2:977093/1‑150 (MQ=255)
                     aaaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTc                                                                                                                                    >  4:791506/1‑149 (MQ=255)
                      aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGc                                                                                                                                 >  4:512676/1‑151 (MQ=255)
                        gCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                               >  1:30277/1‑151 (MQ=255)
                          ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                               >  4:479341/1‑149 (MQ=255)
                          ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                              >  4:901234/1‑150 (MQ=255)
                              gCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  1:11927/1‑151 (MQ=255)
                              gCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         >  4:164311/1‑151 (MQ=255)
                               cccAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                        >  1:463402/1‑151 (MQ=255)
                                   ggctaaaaaTAAAGCGAAATTGTTGT‑CAGCCAGTCCCACCCATACCCCCCCCCAAA‑CAATGCCGGGGTGGCCCCCGGGAAAAGAATAATGCGGGAGGCAAATTTTGCGGGAA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                    <  2:367360/145‑1 (MQ=255)
                                   ggCTGCAAATACAGAAAATTTGTTTT‑CCGCCAGTTCCACCCGGCCCCCCCCCCAAC‑CCATGACGCGGTGGCCGCCGGAAAAAAAATAATACTGGAAGGGAAGTTTGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                                     <  2:504576/150‑1 (MQ=255)
                                    ggcacaaaacacaggAAATTTTTGGCCAGCCTGTTCCACCCCG‑ACCCCCCCCGAACCCATGCCGGGATGGTCGCGGGAAAAAAAATAATTTGGGAAGAAAAGAGGGCGGGAA‑TCCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         <  2:1554997/132‑1 (MQ=255)
                                    gCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   >  3:371189/1‑151 (MQ=255)
                                       gCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAATCAGTACCGCCCCCGAA‑CCATGACGCGGTGGTCGCCGGGAACAGAATAATAATGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                >  4:1035632/1‑151 (MQ=255)
                                       gCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                >  2:603220/1‑151 (MQ=255)
                                         aaaaACAGCGAATTTGTTCG‑CAGCCGTTCCCACCCAGCCCCCCCCCCGAC‑CCGTGAGGCGAGGGTCCCGGGGAAGAGAAGAAGACTGGAACCGATGTTGGCCGGGC‑TCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   <  4:1664316/146‑1 (MQ=255)
                                         aaaTACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                              >  4:1245406/1‑151 (MQ=255)
                                         aaaTAAAGCAAATTTTTTTC‑CACCCGGTCCCAACCAGCCCCCCCCCCGAA‑CCAGGCCGCGTGGGTCGCCGGAAAAAAAAAAATTCTGGAAGCGAAGATGGCGGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  <  4:207395/147‑1 (MQ=255)
                                             aCAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                          >  1:286321/1‑151 (MQ=255)
                                                gCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:1122888/1‑150 (MQ=255)
                                                gCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  4:1637596/1‑150 (MQ=255)
                                                 cGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  3:52479/1‑149 (MQ=255)
                                                   aaaTTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    >  1:204509/1‑151 (MQ=255)
                                                        gttgt‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                                >  4:457380/1‑150 (MQ=255)
                                                         ttgt‑CAGCCAGTTCCACCCAGTCCCCCCCCCAAA‑CCATGACGCGATGGCCGCGGGAAACAGAAAAATACGGGAAGCGAAGATTGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                                 <  4:1206485/148‑1 (MQ=255)
                                                         ttgc‑CAGCCAGTCCCACCCAGTCCCCCCCCCGAC‑CCATGACGGGATGGTCGCGGGAAAAAAAATAATACTGGAAGCGAAGTTTGCCGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                                 <  3:34308/148‑1 (MQ=255)
                                                         ttct‑CACCCATTTCCACCCAGTCCCGCCCCCAAA‑CCATGACGCGATGGTCGCCGGAAACAGAATAATACTGGAAGCGAAGTTGGCGGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               <  4:1039996/147‑1 (MQ=255)
                                                          tgt‑CAGCCAGTTCCAACCAGTCCCCCCCCCGAC‑CCATGCCGGGAGGGCCGCGGGAAACAAAATAATACTGGAGGGGAAGATGGCCGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                              <  3:204523/150‑1 (MQ=255)
                                                          tgt‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                              >  1:155513/1‑150 (MQ=255)
                                                           gt‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                                                                            >  3:466912/1‑151 (MQ=255)
                                                              cggCCATTACCAACAGGTCCCGCCCCCGAC‑CCATGGGGGAATGGTCGCGGGAAAAAGAATAATACGGGAAGAGAAGGTTGGGGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                              <  4:1353051/145‑1 (MQ=255)
                                                              cAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                          >  4:1611946/1‑151 (MQ=255)
                                                                  cAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                      >  3:68024/1‑151 (MQ=255)
                                                                    gTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     >  1:133755/1‑150 (MQ=255)
                                                                        cAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                >  1:192095/1‑151 (MQ=255)
                                                                        cAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                >  2:1395319/1‑151 (MQ=255)
                                                                         acccagccccccccccAAC‑CCAGGCCGGGATGGTCGCGGGAAAAAAAAAAATACGGAAAGCAAAGTTGGCGGGTA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                   <  1:436853/136‑1 (MQ=255)
                                                                         acccAGTCCCCCCCCCAAC‑CCATGACGCGATGGCCGCGGGGAAAAAAATAATACTGGAAGCAAAGATGGCGGGAA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                        <  4:1516917/140‑1 (MQ=255)
                                                                         aaCCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                      >  1:178872/1‑144 (MQ=255)
                                                                         aaCCAGCCCCCCCCCCGAA‑CCATGCCGCGATGGCCGCCGGAAAAAAAAAAATACTGGAAGGGAAGATTGCCGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                               <  4:785712/151‑1 (MQ=255)
                                                                           ccAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                             >  4:984166/1‑151 (MQ=255)
                                                                                cccccccccAAC‑CCATGGCGCGTGGGTCCCGGGAAACAAAAAAATACTGGAAGCGAGGTTTGCGGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                         <  4:1407121/146‑1 (MQ=255)
                                                                                 ccccccccGAA‑CCATGACGCGATGGTCGCCGGAAAAAAAATAATCCTGGAAGCGAAGATTGCCGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                         <  4:221200/146‑1 (MQ=255)
                                                                                 ccGCCCCCGAA‑CAAAGACGCGTTGGACGCCGGAAACAAAATAATACTGGAAGCAAATATTCCGGTAA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                          <  1:110991/148‑1 (MQ=255)
                                                                                  cccccccGAA‑CCAGGGCGCGTTGGTCGCGGGGAAAAAAATAATGCGGGAAGGGAAGATTGCGGGTT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      <  1:103057/149‑1 (MQ=255)
                                                                                   cccccaaaa‑CCAGGGCGGGATGGTCGCCGGAAAAAAAATAATACTGGAAGGGAAGATGGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     <  2:597560/144‑1 (MQ=255)
                                                                                      cccGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                  >  2:1368344/1‑151 (MQ=255)
                                                                                            cctaTGGCGCGATGGCGCCCGGGAAAAAAATAATACGAAAGGAAAAGATGGCGGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             <  2:318036/148‑1 (MQ=255)
                                                                                             ccATGCCGCGATGGTCGCGGGAAAAAAAATAATACTGAAAGCAAAGTTGGCGGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             <  2:1262699/150‑1 (MQ=255)
                                                                                             ccATGACGCGATGGCCGCGGGAAAAAAAATAATACTGGAAGCAAATATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                               <  4:1462489/148‑1 (MQ=255)
                                                                                               aggcggcgATGGTCGCGGGAAAAAAAAAAATACTGGAAGCGAAGTTTGCCGGTA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                            <  2:971641/144‑1 (MQ=255)
                                                                                                  aCGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                       >  1:411731/1‑151 (MQ=255)
                                                                                                  aCGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                       >  4:389645/1‑151 (MQ=255)
                                                                                                   cgcgATGGTCGCCGGAAAAAAAAAAATACTGGAAGGGAAGATGGCGGGTA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                       <  2:1371944/150‑1 (MQ=255)
                                                                                                    gcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                       >  4:498300/1‑149 (MQ=255)
                                                                                                    gcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                     >  2:1158834/1‑151 (MQ=255)
                                                                                                      gttGGCCGCCGGAAAAAAAATAATGCTGGAAGCGAATTTTGCGGGTA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                    <  1:314920/148‑1 (MQ=255)
                                                                                                      gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                    >  1:288978/1‑150 (MQ=255)
                                                                                                      gATGGTCGCCGGAAAAAAAAAAATACGGGAAGCGAAGTTTGCCGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                        <  4:179031/146‑1 (MQ=255)
                                                                                                        tGGTCGCGGGAAACAAAAAAATACTGGAAGCGAAGTTTGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGa                                                   <  4:255565/149‑1 (MQ=255)
                                                                                                         ggTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                                 >  4:656865/1‑150 (MQ=255)
                                                                                                         ggTCGCCGAAAAAAAAATAATACTGGAAGCGAAGTTTGCCGGAA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                    <  4:1352887/147‑1 (MQ=255)
                                                                                                              cgggaaaaaGAAAAATACTGGAGGCGAAGATGGCGGTGA‑TCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            <  2:579076/142‑1 (MQ=255)
                                                                                                               cGGAAACAAAATAATACGGGAAGCAAAGATTGCCGGTT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            <  4:1043781/149‑1 (MQ=255)
                                                                                                                gggAACATAATAATACGGGAAGCAAAGATTACGGGGA‑TCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                          <  4:1487795/150‑1 (MQ=255)
                                                                                                                 ggAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                        >  4:691734/1‑151 (MQ=255)
                                                                                                                   aaaaaaataatACAAAAAGCGAAGTTTGCGGGAA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                      <  2:493150/146‑1 (MQ=255)
                                                                                                                   aaCAAAAAAATACGGGAAGCGAAGATTGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       <  4:202398/150‑1 (MQ=255)
                                                                                                                      aaaataatGCGGGAGGCTAGGATGGCGGGAA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  1:360055/149‑1 (MQ=255)
                                                                                                                       gAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  1:146163/1‑151 (MQ=255)
                                                                                                                        aataatACGGGAAGCGAAGATTGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 <  4:172702/151‑1 (MQ=255)
                                                                                                                           aataCTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               >  2:1165616/1‑150 (MQ=255)
                                                                                                                                 ggAAGCGAAGTTTGCGGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        <  2:511967/151‑1 (MQ=255)
                                                                                                                                 ggAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        >  2:1522960/1‑151 (MQ=255)
                                                                                                                                 ggAAGAAAAGTTTCCCGGGT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                          <  4:355514/149‑1 (MQ=255)
                                                                                                                                 gaaaGAGAAGTTTGCGGGTA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                          <  2:587287/147‑1 (MQ=255)
                                                                                                                                  gAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                       >  4:1268050/1‑151 (MQ=255)
                                                                                                                                   aaGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                      >  4:521074/1‑151 (MQ=255)
                                                                                                                                      ggaaagttGCCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                       <  2:1446903/142‑1 (MQ=255)
                                                                                                                                       gAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  >  3:16438/1‑151 (MQ=255)
                                                                                                                                        atggttGCGGCGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  <  2:790464/146‑1 (MQ=255)
                                                                                                                                        aaGATTGCGGGAT‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  <  2:806432/150‑1 (MQ=255)
                                                                                                                                         aGATTGCCGGGA‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                <  4:908825/151‑1 (MQ=255)
                                                                                                                                         aGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                >  2:1212158/1‑151 (MQ=255)
                                                                                                                                           aTTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa               >  4:178823/1‑150 (MQ=255)
                                                                                                                                           aTTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa               >  1:68312/1‑150 (MQ=255)
                                                                                                                                              gcgggta‑TTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa               <  2:1029540/141‑1 (MQ=255)
                                                                                                                                              gCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat            >  2:107520/1‑150 (MQ=255)
                                                                                                                                               cggggA‑TCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat            <  3:259775/147‑1 (MQ=255)
                                                                                                                                                   gA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat      >  4:1152367/1‑151 (MQ=255)
                                                                                                                                                       tACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact   >  1:295131/1‑151 (MQ=255)
                                                                                                                                                        aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  4:1015883/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGT‑CAGCCAGTTCCAACCAGTACCGCCCCCGAA‑CCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGA‑TTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑