Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913213,3410AG40.9% 189.5 / 40.0 116G337G (GGA→GGG) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (9/59);  new base G (47/0);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.66e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213191‑213491
                                                                                                                                                      |                                                                                                                                                        
aGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGa                                                                                                                                                          <  2:1356517/151‑1 (MQ=255)
  aTGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAg                                                                                                                                                        <  4:620095/151‑1 (MQ=255)
     ccTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgtgt                                                                                                                                                     <  4:630368/151‑1 (MQ=255)
     ccTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgtgt                                                                                                                                                     <  4:1206511/151‑1 (MQ=255)
        cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAgtg                                                                                                                                                      >  4:855351/1‑147 (MQ=255)
             tGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGTaaa                                                                                                                                                  >  1:244367/1‑144 (MQ=255)
              gTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTaa                                                                                                                                            <  4:1550441/151‑1 (MQ=255)
                  aCGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGATGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGCCTAATGc                                                                                                                                         >  2:960219/1‑150 (MQ=255)
                   cGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCgg                                                                                                                                       >  1:343502/1‑151 (MQ=255)
                    gTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGGAGGGGACAGCCTCATGCggg                                                                                                                                      >  2:309951/1‑151 (MQ=255)
                     tttGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCggg                                                                                                                                      <  2:747194/150‑1 (MQ=255)
                       tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAg                                                                                                                                    <  1:229071/150‑1 (MQ=255)
                       tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAg                                                                                                                                    <  4:1393791/150‑1 (MQ=255)
                       tGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAg                                                                                                                                    <  2:1357172/150‑1 (MQ=255)
                        gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAgg                                                                                                                                   <  2:790604/150‑1 (MQ=255)
                        gggCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGc                                                                                                                                  <  1:326773/151‑1 (MQ=255)
                           cgcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGTGGAGAGGGCAGCTTTAtcagtggggtt                                                                                                                                 >  4:642376/1‑139 (MQ=255)
                            gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGGGTGTAACGCGTAAAGCgggggggcgt                                                                                                                               >  2:731726/1‑146 (MQ=255)
                            gcgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGATTAATgggggggggg                                                                                                                                 >  2:70171/1‑146 (MQ=255)
                             cgTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGatat                                                                                                                             <  3:48986/151‑1 (MQ=255)
                              gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTCGGGAGGCGATAtt                                                                                                                            >  4:1001244/1‑151 (MQ=255)
                              gTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAAGGTACAGCTTAATGCGGGAGGGGATAtt                                                                                                                            >  3:77769/1‑151 (MQ=255)
                                ttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTc                                                                                                                           <  1:25221/150‑1 (MQ=255)
                                 tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGCGGGGGGCGATAtt                                                                                                                            >  4:338019/1‑148 (MQ=255)
                                 tGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGCCGGGTACCGCTTAAATCGGGGGGc                                                                                                                                  >  4:186846/1‑142 (MQ=255)
                                    aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGGCAACCTAATAAGGGAGGGGGTAtt                                                                                                                            >  4:639065/1‑145 (MQ=255)
                                    aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCt                                                                                                                      <  2:435769/151‑1 (MQ=255)
                                       ccGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTcc                                                                                                                    <  3:415255/150‑1 (MQ=255)
                                         gACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGGGGGGAGGGGACAGCTTAAATCGGGGGGGGGTATTCGcccccccc                                                                                                                  >  2:1070777/1‑148 (MQ=255)
                                            gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTAAAGCTTTATGCGGGGGGGGGTATTCTCCCCCCCCGTg                                                                                                               >  2:1177479/1‑150 (MQ=255)
                                            gCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCGTAAAGCGGGGGGCGATATTCTGCCTcccc                                                                                                                  >  4:1410342/1‑145 (MQ=255)
                                              tATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGGTAttttcccccccc                                                                                                                  >  2:339172/1‑135 (MQ=255)
                                               aTCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAg                                                                                                            <  2:626255/150‑1 (MQ=255)
                                                 cAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAAGCGGGGGGGGGTATTTGcccccccc                                                                                                                  >  1:300060/1‑140 (MQ=255)
                                                  tgTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGGGTACAGCCTAATGCGGGAGGCGGTATTCGCCCTCCCCCTGCAGACg                                                                                                         >  2:428797/2‑150 (MQ=255)
                                                  aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGGGTACAGCTTAATGCGGGGGGGGGTATTTGCCCCCCGCCTGCAg                                                                                                            >  2:104322/1‑147 (MQ=255)
                                                   gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGaa                                                                                                       <  2:580325/151‑1 (MQ=255)
                                                   gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGaa                                                                                                       <  2:452655/151‑1 (MQ=255)
                                                    tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGGGAGGAAAGCGTAATGGGGGGGGCGGTATTTGCCCGCCCCGGGCGGaag                                                                                                         >  4:84790/1‑146 (MQ=255)
                                                     cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAg                                                                                                      <  4:1413952/150‑1 (MQ=255)
                                                          cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCCGGGGAGAGGACACCTTTATTCGGGGGGGGAGATTCGCCCTCCCCGGGCAGACGCAGAgggt                                                                                                 >  1:364926/1‑148 (MQ=255)
                                                            gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAg                                                                                               <  4:1090174/150‑1 (MQ=255)
                                                               gtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGNNNAgc‑gtg                                                                                          <  2:737067/151‑1 (MQ=255)
                                                                 ggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAgc‑gtgc‑g                                                                                       <  1:384003/151‑1 (MQ=255)
                                                                   aTTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGTGTACAGCTTAAAGCGGGGGGCGGTATTCGCCCTCCGCGTGCAGACGCAATGGGCAgc‑gtgc‑g                                                                                       >  4:124455/1‑149 (MQ=255)
                                                                    ttAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGAAAGCTTAATGCGGGGGGGGGTATTTGCCCCCCCCGTGCAGACGAAGAGGGCAGC‑GGGC‑Ggtt                                                                                    >  2:434429/1‑151 (MQ=255)
                                                                      aaaTCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTc                                                                                   <  4:1527613/150‑1 (MQ=255)
                                                                         tCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTTAAGCGGGAGGGGATATTTGCCCTCCCCGTGGAGACGAAGCGGGCAGC‑GTGC‑GGTTcca                                                                                 >  2:976183/1‑147 (MQ=255)
                                                                          ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGAGACCTTACAGCGGGGGGGGGTATTCGCCCCCCCCCTGGAGACGGAGCGGTGAGG‑GTGG‑GTGTTaaa                                                                                >  1:315901/1‑149 (MQ=255)
                                                                          ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAgc                                                                              <  2:1650392/151‑1 (MQ=255)
                                                                              cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCa                                                                          <  2:1005711/151‑1 (MQ=255)
                                                                                ccGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAgg                                                                        <  2:1357923/151‑1 (MQ=255)
                                                                                 cGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGa                                                                       <  4:1011701/151‑1 (MQ=255)
                                                                                  gggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCTTAATGCGGGAGGGGATATTTCCCCTCCGCGGGCAGACGAAGCGGGCAGG‑GGGC‑GGTTTAAAACGCCAgg                                                                        >  2:58618/1‑149 (MQ=255)
                                                                                  gggCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGAt                                                                      <  2:1552822/151‑1 (MQ=255)
                                                                                     cAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAACTTAATTCCGGGGGGGACATTTTCCCCCCCCGGGGAGGCGGAGGGGGCCGG‑GGGCGGTTTCAAA‑CGCCAggggt                                                                     >  2:861698/1‑146 (MQ=255)
                                                                                      aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgct                                                                  <  3:335491/151‑1 (MQ=255)
                                                                                       aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGCGGTATTCCCCCCCCCCGTGCAGACGAAGCGGGCAGC‑GTGC‑GGTTCTAAGCGCCAGGATtgct                                                                  >  2:209259/1‑150 (MQ=255)
                                                                                       aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgctg                                                                 <  4:1155652/151‑1 (MQ=255)
                                                                                        aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATtgctgc                                                                <  1:270303/151‑1 (MQ=255)
                                                                                          cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGAAGAGTAAAGCTTAATACGGGGGGCGATATTCGCCCTCCCCGGGCAGAAGAAACGGGCAGC‑GGGC‑GGTTCAAAACGCCAGGATTGCTGCat                                                              >  4:128050/1‑151 (MQ=255)
                                                                                          cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCATAATGCGGGAGGCGGGATTCGCCCTCCGCGGGGCGACGAAGAGGGCAGC‑GCGG‑GGTTCAAAGAGCCAGGGTtgttgcct                                                              >  4:1340493/1‑149 (MQ=255)
                                                                                          cGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCat                                                              <  4:612316/151‑1 (MQ=255)
                                                                                            aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGCGGGGGGGGGTATTCGCCCCCCGCGTGCAGACGAAGCGGGCAGC‑GGGC‑GGTTCAAAGCGCCAGGATTGGTGCat                                                              >  4:1321830/1‑149 (MQ=255)
                                                                                            aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCatat                                                            <  3:464121/151‑1 (MQ=255)
                                                                                               aCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTAAAGCTTTATGCGGGGGGGGGTATTTGCCCTCCCCGTGCAGACGAAGCGGTCAGC‑GGGG‑GTTTCAAAGCGCCAGGATTGCTGCATAtttt                                                         >  1:122879/1‑149 (MQ=255)
                                                                                                cATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTc                                                        <  2:791621/151‑1 (MQ=255)
                                                                                                  ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGGGGGGATATTCTCCCCCCGCGGGCAGACGAAGCGGGCAGC‑GGGC‑GTTTCAAAGCGCCAGGATTGGTGCCTATTTTCgg                                                      >  3:137157/1‑151 (MQ=255)
                                                                                                     tgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGGCAGATTAATGCGGGGGGCGCTAATCGCCCTCCCCCGGCAGAAGAAACGGGCGGC‑GCGC‑GCTTCCAAACGGCAGGGCTGCTGCATCATTTCggg                                                     >  2:1338310/1‑149 (MQ=255)
                                                                                                      gCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGt                                                  <  4:1576667/151‑1 (MQ=255)
                                                                                                         gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAc                                               <  4:1238161/151‑1 (MQ=255)
                                                                                                            gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                            <  4:1519116/151‑1 (MQ=255)
                                                                                                             cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTTCAGCTTTATGCGGGAGGCGATATTTGCCCCCCCCGTGCAGACGAAGCGGGCAGC‑GGGC‑GTTTCAAAACGCCCCGGGTGCTGCATATTGTCGGGCGGAAcg                                              >  4:83594/1‑148 (MQ=255)
                                                                                                             cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAATGCGGGGGGGGATATTTTCCCCCCCCGGGCAGACGAAGCGGGCAGG‑GTGG‑GGTTTAAAGCGCCCGGATTGCTGCCTATTTTCgggggggaagg                                             >  2:1191992/1‑141 (MQ=255)
                                                                                                               gACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGAGTACAGCTTAATGCGGGGGGGGGTAATTACCCCCCCCGCGCAGACGAAAGGGGGAGC‑GTGC‑GGTTTAAAACGCCAGGGTTGCTGCCTATTGTCGGGCGGAAcggcgg                                          >  4:208556/1‑150 (MQ=255)
                                                                                                                 cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACg                                       <  3:228567/151‑1 (MQ=255)
                                                                                                                 cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACg                                       <  2:625058/151‑1 (MQ=255)
                                                                                                                   tGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGTCAGCTTAATGCGGGGGGCGGTATTCTCCCTCCCCGTGGCGACGAAAGGGGCAGC‑GGGG‑GTTTTCAAACGCCACGATTGCTGGCTATTGTTGGGCGtgagcgcggg                                         >  2:1196133/1‑138 (MQ=255)
                                                                                                                    ggCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTTATTCGGGAGGGGGTATTTGCCCCCCGCGGGGAGACGAAGCGGGCAGCGGGGG‑GTTTCAAA‑CGCCAGGGATGGTGCCTATTTTCGGGCGGAAaggcggg                                         >  4:1042758/1‑145 (MQ=255)
                                                                                                                        tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGc                                  <  3:56716/149‑1 (MQ=255)
                                                                                                                        tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGc                                  <  4:396993/149‑1 (MQ=255)
                                                                                                                           aCCGCTGGAATTACCGGCGGGGCTGGGGAGAGGACAGCTTAATGCGGGGGGCGATATTTGCCCCCCGCGTGCCGACGAAACGGTCAGC‑GGGC‑GGTTCAAAACGCCAGGATTGCTGCATATTTTCGGGCGTAACGGCGGGCGGCAGCTaaa                              >  1:444352/1‑150 (MQ=255)
                                                                                                                             cGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGCGGGAGGGGATATTCGCCCTCCGCGTGCAGACGAAACGGGCAGC‑GTGC‑GTTTTAAAACGCCAGGATTGCTGCATATTGTCGGGGGTAACGGCGGGCGTAAGCTaaa                              >  3:407390/1‑148 (MQ=255)
                                                                                                                                 ggAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGaaaa                         <  4:1191230/149‑1 (MQ=255)
                                                                                                                                  gAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCt                      <  1:387770/151‑1 (MQ=255)
                                                                                                                                    aTTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTgg                    <  2:115875/151‑1 (MQ=255)
                                                                                                                                         cggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGAAACGGCGGACGTAAGCTAAAGAAAATCTGGCAaga               <  2:1104411/151‑1 (MQ=255)
                                                                                                                                          ggcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAagag              <  4:1357961/151‑1 (MQ=255)
                                                                                                                                           gcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAagaga             <  2:1408581/151‑2 (MQ=255)
                                                                                                                                           gcggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGc             >  2:705005/1‑151 (MQ=255)
                                                                                                                                            cggGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCt            <  3:52683/151‑1 (MQ=255)
                                                                                                                                              gggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg          >  2:408090/1‑151 (MQ=255)
                                                                                                                                               ggCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTggg         >  4:1503889/1‑151 (MQ=255)
                                                                                                                                                gCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGc        <  4:70304/151‑1 (MQ=255)
                                                                                                                                                gCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGc        >  4:807252/1‑151 (MQ=255)
                                                                                                                                                   gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGt      <  2:963350/150‑1 (MQ=255)
                                                                                                                                                   gggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg     <  4:104498/151‑1 (MQ=255)
                                                                                                                                                    ggAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgc    >  4:1062531/1‑151 (MQ=255)
                                                                                                                                                      aaGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccg  >  1:253903/1‑151 (MQ=255)
                                                                                                                                                      aaGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTgccg  >  3:434399/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGC‑GTGC‑GTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCG  >  NC_000913/213191‑213491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑