Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG44.0% 200.4 / 34.3 116V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (9/56);  new base G (51/0);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 6.31e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354138‑354437
                                                                                                                                                       |                                                                                                                                                       
aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGt                                                                                                                                                         <  3:149299/151‑1 (MQ=255)
   ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGa                                                                                                                                                       <  4:518647/150‑1 (MQ=255)
    gggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGaaa                                                                                                                                                     <  3:239516/151‑1 (MQ=255)
       cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGAt                                                                                                                                                  <  1:358170/151‑1 (MQ=255)
        aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATg                                                                                                                                                 <  4:551762/151‑1 (MQ=255)
            gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCt                                                                                                                                             <  1:210227/151‑1 (MQ=255)
             tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATgcg                                                                                                                                               >  3:412211/1‑148 (MQ=255)
              gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑g                                                                                                                                           <  4:1425635/150‑1 (MQ=255)
                cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGCT‑gga                                                                                                                                         >  4:631226/1‑148 (MQ=255)
                cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTAGCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑Gaaa                                                                                                                                        <  3:330868/151‑1 (MQ=255)
                 gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAg                                                                                                                                       <  4:1292690/151‑1 (MQ=255)
                 gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAg                                                                                                                                       <  4:1155411/151‑1 (MQ=255)
                       ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GCAAggggggg                                                                                                                                 >  2:1403559/1‑147 (MQ=255)
                       ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTTGGGGGGAAGATGCGCT‑GAAAGGGCCgg                                                                                                                                 >  3:265254/1‑151 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtg                                                                                                                               <  2:718169/150‑1 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtg                                                                                                                               <  2:385027/150‑1 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtg                                                                                                                               <  4:1416419/150‑1 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGTGAAAGATGCGGTGGAAAGGGCggggg                                                                                                                               >  1:193405/1‑149 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGC‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGGAggggg                                                                                                                              >  4:701342/1‑151 (MQ=255)
                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcg                                                                                                                            <  1:107877/150‑1 (MQ=255)
                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGCG‑GGAAGGGGAgggggggg                                                                                                                           >  2:1236713/1‑151 (MQ=255)
                                  tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGACGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtggcggtggc                                                                                                                       <  4:1030874/150‑1 (MQ=255)
                                     tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCT‑GATAGGGGcgggggcggggggggtt                                                                                                                   >  2:171741/1‑146 (MQ=255)
                                      ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑gtt                                                                                                                  >  3:105619/1‑150 (MQ=255)
                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                                                                               <  4:254585/151‑1 (MQ=255)
                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                                                                               <  4:753856/151‑1 (MQ=255)
                                            cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAGGGCCGGGGGCGGGGGCG‑TTTGTCAttt                                                                                                           >  4:1304573/1‑150 (MQ=255)
                                             gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTc                                                                                                           <  1:403576/150‑1 (MQ=255)
                                              cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCT‑GAAAGGGGAGGTGGCGGGGGCG‑TTTGTCCTTcc                                                                                                          >  4:163672/1‑150 (MQ=255)
                                               aCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGa                                                                                                        <  3:339346/151‑1 (MQ=255)
                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAaga                                                                                                    <  4:729140/151‑1 (MQ=255)
                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGCT‑GGAAGGGCGGGGGGCGGGGGGG‑GTTGTCATTCCGaaa                                                                                                      >  2:746352/1‑149 (MQ=255)
                                                     gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAagaga                                                                                                  <  4:130885/151‑1 (MQ=255)
                                                     gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAagaga                                                                                                  <  4:878380/151‑1 (MQ=255)
                                                       tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAGATGGCGT‑GAAAGGGCAGGGGGCGGGGGGG‑GTTTTCCTTCCGAAaggggg                                                                                                 >  4:447996/1‑147 (MQ=255)
                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGa                                                                                              <  4:357166/150‑1 (MQ=255)
                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑AGATGGGGGGCAAACTGCGCT‑GGAAAGGGAGGTGGGGGTGGCGTTTTGTCA‑TCCGaaa                                                                                                      >  4:297831/1‑142 (MQ=255)
                                                           aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACa                                                                                            <  2:530122/151‑1 (MQ=255)
                                                              aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGt                                                                                          <  2:555237/150‑1 (MQ=255)
                                                              aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGt                                                                                          <  2:33562/150‑1 (MQ=255)
                                                               aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                        <  1:300946/151‑1 (MQ=255)
                                                               aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                        <  2:93335/151‑1 (MQ=255)
                                                               aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGGT‑GAAAGGGGAGGGGGGGGGGGGG‑TTTGTCATTTCGAAAGAGGGCGACAGTCt                                                                                        >  4:1657587/1‑151 (MQ=255)
                                                                cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAAATGGGCT‑GAAAGGGGAGGGGGGGGGGGGG‑GTTGTGATTCCGAAAGAGAGCGGCAGGCt                                                                                        >  2:435933/1‑150 (MQ=255)
                                                                   tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                     <  1:335302/150‑1 (MQ=255)
                                                                    gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                      >  4:392850/1‑148 (MQ=255)
                                                                    gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGT‑GCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                     >  2:612047/1‑148 (MQ=255)
                                                                      cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGGT‑GGAAGGGGCGGGGGCGGGGGCG‑TTTGTCATTCCGGAAGAGAGGGGCAGGCTGGAAGa                                                                                  >  1:459787/1‑150 (MQ=255)
                                                                       gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                                <  4:1587632/151‑1 (MQ=255)
                                                                             aggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGC‑GGTTGGGGGGAAAAATGCGCC‑GCAAGGGCGGGGGGCGGGGGCG‑TTTGTCAATTCGCAAGAGAGGGACCGGCTGGGAGAGCGTgcgg                                                                          >  2:686898/2‑148 (MQ=255)
                                                                              ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                         <  2:553342/151‑1 (MQ=255)
                                                                               gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GCAAAGGCAGGGGGCGGTGGCG‑TTTGTTATTTCGAAAGAGAGCGACAGTCTGGGAGATCg                                                                               >  2:1435578/1‑144 (MQ=255)
                                                                                cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTgg                                                                       >  4:320972/1‑151 (MQ=255)
                                                                                  ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     <  3:66303/151‑1 (MQ=255)
                                                                                   gggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAAATGCGCT‑GAAAGGGCAGGGGGCGGGGGGG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                     >  1:225843/1‑150 (MQ=255)
                                                                                    ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGA‑GGGTGGGGGGGAAGAGGCGCC‑GGAAGGGCCGGGGGGGGGGGCG‑GTTGGCTTTCCGAAAGAGAGCGGCAGTCTGGAAGAGCGTGAtg                                                                          >  2:881189/1‑144 (MQ=255)
                                                                                     gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                                   >  3:173604/1‑150 (MQ=255)
                                                                                     gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGCCGGGGGGGGGGGGG‑TTTGTCATTCCGCAAGAGAGGGGCAGGCTGGGAGATCGTGGTGGGgcg                                                                     >  2:404566/1‑148 (MQ=255)
                                                                                        gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCG‑TTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTGGGGCACTc                                                                >  4:1529677/1‑150 (MQ=255)
                                                                                        gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAAGGCAGGGGGGGGTGGGG‑TTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGAACGTGATGGGGCGCAccc                                                                >  1:7169/1‑148 (MQ=255)
                                                                                         cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                              <  4:417529/151‑1 (MQ=255)
                                                                                         cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAAGGCAGGGGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGc                                                              >  3:378779/1‑151 (MQ=255)
                                                                                          gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                             <  2:1246653/151‑1 (MQ=255)
                                                                                          gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGGGGTGGCG‑TTTTTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               >  2:1393957/1‑149 (MQ=255)
                                                                                          gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAAGGGAGGGGGGGGGGGGG‑GTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGGGCACTCGcc                                                             >  2:1074153/1‑150 (MQ=255)
                                                                                           cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGTGGCGGGGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               >  3:4586/1‑148 (MQ=255)
                                                                                            gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGCTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCaa                                                            <  2:1016924/150‑1 (MQ=255)
                                                                                              gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                         <  1:272729/151‑1 (MQ=255)
                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGGAGGGGGCGGGGGGG‑GTTGTCATTCCGAAAGAGAGGGAAAAGCTGGAAGAGCGTGATGTTGCGCACTc                                                                >  4:1267592/1‑143 (MQ=255)
                                                                                                ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCC‑GGAAGGGGAGGTGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGGGTGGGGCACTCGCAagag                                                         >  1:184783/1‑149 (MQ=255)
                                                                                                 tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGaa                                                       <  3:306504/150‑1 (MQ=255)
                                                                                                 tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCG‑GTTTTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAaga                                                          >  4:865877/1‑147 (MQ=255)
                                                                                                 tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGAAGGGCC‑GGAAAGGGAGGGGGGGGGGGGG‑GTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGccaaaaa                                                        >  1:184209/1‑143 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     <  4:633199/151‑1 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  2:1653416/150‑1 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GGAAGGGCAGGGGGGGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     >  1:276844/1‑151 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGAAGCGCT‑GAAAGGGGAGGGGGCGGGGGGG‑GTTTTCATTTCGGAAAAGAGAGACAGTCTGGGAGATCGGGATGGGGCGCACTAGCAAGAGaa                                                       >  2:481072/1‑149 (MQ=255)
                                                                                                   aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCG‑GTTGTTATTCCGGAAAAGAGCGGCAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                        >  2:405449/1‑147 (MQ=255)
                                                                                                    agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGCAGGGGGGGGGGGGG‑TTTGTCATTTCGAAAGAGAGCGACAGGCTGGAAGATCGGGGTGTGGGGGACTCCCAAGaaaa                                                       >  2:309268/1‑147 (MQ=255)
                                                                                                     gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                   >  4:898718/1‑150 (MQ=255)
                                                                                                        agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAAATGCGCT‑GAAAGGGGAGGGGGCGGGGGGG‑TTTGTCATTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                   >  4:750958/1‑147 (MQ=255)
                                                                                                               ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                            <  2:1474252/147‑1 (MQ=255)
                                                                                                                  gCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggt                                     <  2:1002292/151‑1 (MQ=255)
                                                                                                                   ccATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtg                                    <  2:583405/151‑1 (MQ=255)
                                                                                                                    cATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   <  2:548434/151‑1 (MQ=255)
                                                                                                                    cATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTTGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   >  4:1405489/1‑151 (MQ=255)
                                                                                                                     aTCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGCGCtggtgg                                   >  4:115457/1‑150 (MQ=255)
                                                                                                                      tCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAAATGCGCT‑GAAAGGGGCGGGGGCGGGGGGG‑GTTTTCCTTCCGGAAAAGAGGGACAGTCTGGGAGATTGTGATGTGGCGCACTCGGCAGAGAAGGCGATTATGGCGCtggggg                                   >  2:1413166/1‑149 (MQ=255)
                                                                                                                       ccGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                                 <  1:380979/150‑1 (MQ=255)
                                                                                                                       ccGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                                 <  4:496832/150‑1 (MQ=255)
                                                                                                                             gTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGCG‑GTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGGGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGGAAGcc                             >  2:723707/1‑148 (MQ=255)
                                                                                                                                 ccGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                      <  3:264008/151‑1 (MQ=255)
                                                                                                                                       tGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGCCAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                >  2:1606789/1‑151 (MQ=255)
                                                                                                                                            gt‑ggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  2:600457/1‑151 (MQ=255)
                                                                                                                                               ggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc         >  2:347423/1‑151 (MQ=255)
                                                                                                                                                 ttGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc       >  3:299059/1‑151 (MQ=255)
                                                                                                                                                  tGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg      >  4:891532/1‑151 (MQ=255)
                                                                                                                                                    gggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc    <  2:630379/151‑1 (MQ=255)
                                                                                                                                                    gggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc    <  4:1405313/151‑1 (MQ=255)
                                                                                                                                                      gTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  <  4:803469/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354138‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑