Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913366,5260TG46.4% 160.8 / 51.4 114T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (12/48);  new base G (52/0);  total (66/48)
Fisher's exact test for biased strand distribution p-value = 1.15e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366377‑366675
                                                                                                                                                           |                                                                                                                                                      
aaaGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGggt                                                                                                                                                        <  4:1375785/150‑1 (MQ=255)
 aaGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGggtgg                                                                                                                                                      <  2:32099/151‑1 (MQ=255)
     cTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTACGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGT‑G‑G‑A‑TT‑T‑GGCGGCCGGGGGGgttt                                                                                                                                                  >  4:1252196/1‑151 (MQ=255)
      tGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑ATTT‑G‑GGGGCCGGGGGGGtttt                                                                                                                                                  >  4:21661/1‑151 (MQ=255)
       ggggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGtttt                                                                                                                                                  <  2:878720/149‑1 (MQ=255)
       ggggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGtttt                                                                                                                                                  <  4:512243/149‑1 (MQ=255)
        gggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑A‑TT‑T‑GGGGCCCGGGGGGgtttttt                                                                                                                                               >  4:1543547/1‑149 (MQ=255)
        gggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTttttct                                                                                                                                               <  3:218361/151‑1 (MQ=255)
        gggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTttttct                                                                                                                                               <  2:804569/151‑1 (MQ=255)
          gTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTttttcttt                                                                                                                                             <  1:400455/151‑1 (MQ=255)
            gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑A‑TT‑G‑GGGGCCAGGGGGGGttttttttt                                                                                                                                            >  3:476946/1‑150 (MQ=255)
            gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGG‑G‑T‑ATTT‑G‑GGGGCCGGGGGGGtttttttttt                                                                                                                                            >  4:1569122/1‑151 (MQ=255)
             ccTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGG‑G‑G‑ATTT‑G‑GGGGCCGGGGGGGTtttttttt                                                                                                                                             >  2:101646/1‑149 (MQ=255)
               tAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑G‑TTGG‑GGGGCCGGGGGGGtttttttttttcc                                                                                                                                         >  2:640931/1‑148 (MQ=255)
                aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGG‑A‑A‑TT‑G‑GGGGCCGGGGGGGGTTTTTTTTTcccc                                                                                                                                        >  4:145673/1‑151 (MQ=255)
                 aTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGGGGCAGGGGGGGTTTTCTTTTTAc                                                                                                                                         >  3:301595/1‑148 (MQ=255)
                  tgagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCCGGGGGGTTTTTTTTTTTACCCGt                                                                                                                                     >  2:863743/1‑151 (MQ=255)
                   gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑G‑T‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTAcc                                                                                                                                        >  1:286208/1‑147 (MQ=255)
                   gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGt                                                                                                                                     <  4:1081819/150‑1 (MQ=255)
                      tgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑G‑T‑TT‑T‑GGCGCCCGGGGGGGTTTTCTTTTTAcc                                                                                                                                        >  1:254931/1‑144 (MQ=255)
                       gagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGG‑G‑C‑A‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTAc                                                                                                                                         >  2:1264873/1‑142 (MQ=255)
                        agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGG‑GTT‑T‑TT‑G‑GGGGCCGGGGGGGTTTTTTTTTTTACCAgggg                                                                                                                                   >  1:130021/1‑145 (MQ=255)
                         gCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACgg                                                                                                                              <  4:1210003/151‑1 (MQ=255)
                         gCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑G‑A‑TT‑G‑GGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACgg                                                                                                                              >  4:391190/1‑151 (MQ=255)
                          cTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑TGA‑TT‑G‑GGGCCCGGGGGGGtttttttttttccctggaggaggg                                                                                                                              >  2:1071267/1‑137 (MQ=255)
                           tAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTG‑G‑G‑A‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGAAggg                                                                                                                             >  4:861338/1‑150 (MQ=255)
                           tAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGACCAACGCGCGGGGAGAGGCGGCTTGC‑G‑G‑A‑TT‑T‑GGGGCCAGGGAGGGTTTTCTTTTTCACAGTGAGACggg                                                                                                                             >  4:1334321/1‑150 (MQ=255)
                            aaCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTT‑G‑T‑T‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTTCCCGTGAGACggg                                                                                                                             >  4:1172587/1‑149 (MQ=255)
                             aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCaa                                                                                                                          <  1:114118/151‑1 (MQ=255)
                             aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑G‑A‑TT‑T‑GGGGCCAGGGGGGGTTTTTTTTTTACCCGTGAGACggg                                                                                                                             >  3:264526/1‑148 (MQ=255)
                               tCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTC‑G‑T‑A‑TT‑T‑GGCGGCAGGGGGGGTTTTTTTTTTACCAGCGAGACggg                                                                                                                             >  2:1642984/1‑146 (MQ=255)
                                 acaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGc                                                                                                                      <  4:1616019/151‑1 (MQ=255)
                                   aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTG‑G‑T‑A‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTTCCCGTGAGACGGGCaaaa                                                                                                                        >  4:1512313/1‑145 (MQ=255)
                                   aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTg                                                                                                                    <  4:637404/151‑1 (MQ=255)
                                   aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTg                                                                                                                    <  2:438808/151‑1 (MQ=255)
                                    ttAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑G‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTTCCAGTGAGACGGGGGACAGCTg                                                                                                                    >  2:367363/1‑150 (MQ=255)
                                             ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCCGGGGGGGTTTTTTTTTTTCCAGTGAGAAGGGGAACAACTGATTGgcc                                                                                                             >  2:452574/1‑148 (MQ=255)
                                               gcgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAc                                                                                                        <  3:9857/151‑1 (MQ=255)
                                                 gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑G‑A‑TT‑T‑GGGGCCAGGGGGGGTTTTTTTTTTACCAATGAGAAGGGCAACAGCTCATTGCCCtt                                                                                                           >  4:120472/1‑146 (MQ=255)
                                                  cTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGGGCCAGGGGGGGTTTTTTTTTTACCAGGGAGACGGGCAACAGCCGATTGCCCCTCCCCGc                                                                                                     >  3:450585/1‑151 (MQ=255)
                                                    cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCt                                                                                                   <  2:1506288/151‑1 (MQ=255)
                                                     aCTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCAGGGGGGGTTTTTTTTTTACCCGTg                                                                                                                                    >  4:256696/1‑117 (MQ=255)
                                                     aCTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACg                                                                                                                               <  2:256284/122‑1 (MQ=255)
                                                     aCTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTg                                                                                                  <  4:285482/151‑1 (MQ=255)
                                                       tGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGATTTT‑G‑T‑A‑TT‑T‑GGCGGCACGGGGGGTTTTTTTTTCACCAAGGGGAGGGGAAAAAAATGATTGTCCTTCCCCGcc                                                                                                    >  4:1554446/1‑147 (MQ=255)
                                                          ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCTCCAGGGTGGTTTTTCTTTTCGCCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCt                                                                                             <  4:498190/151‑1 (MQ=255)
                                                            gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTga                                                                                           <  2:1669926/151‑1 (MQ=255)
                                                              tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑G‑A‑TT‑T‑GGCGGCAGGGGGGGTTTTCTTTTTACCCGTGAGAAGGGCAAAAGCTGATTGCCCTTCACCGCCTGGccc                                                                                              >  1:441215/1‑146 (MQ=255)
                                                                 ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGGGGCCGGGGGGTTTTTTTTTTCACCAGGGAGACGGGGAACAGCTGATTTCCCTTCACCGCCTGGCCCTgaggg                                                                                        >  3:41143/1‑147 (MQ=255)
                                                                   aGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGtt                                                                                    <  2:1044497/151‑1 (MQ=255)
                                                                    gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTg                                                                                   <  2:933430/151‑1 (MQ=255)
                                                                      cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑A‑TT‑T‑GGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAACAGCTGGTTGCCCTTCACCCCCTGGCCCTGGGAGGGTTg                                                                                   >  1:287486/1‑149 (MQ=255)
                                                                      cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgc                                                                                  <  4:809783/150‑1 (MQ=255)
                                                                                 tCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCCGGGGGGGTTTTTTTTTTACCCGGGAGAAGGGCAACAACTGATTGCCCCTCACCCCCCGGCCCCGCGAGAGGTGCAGC‑AAGCGGGcc                                                                     >  2:943766/1‑151 (MQ=255)
                                                                                  cGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTTCCAGGGAGACGGGCAACAGCTGATTGCCCCTCACCCCCCGGCCCCGAGAGAGGTGCAGC‑AAGCGGGcc                                                                     >  2:1055521/1‑150 (MQ=255)
                                                                                  cGTGCCAGCTGCATTAATGAATCGGCAAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTcc                                                                     <  2:453901/150‑1 (MQ=255)
                                                                                   gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑A‑TT‑T‑GGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCCGGCCCCGAGAGAGGTGCAGC‑AAGCGGGCCAc                                                                   >  4:761914/1‑151 (MQ=255)
                                                                                           tGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑A‑TT‑T‑GGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAACAGCTGATTGCCCTTCACCCCCTGGCCCCGAGAGAGGTGCAGC‑AAACGGGCCACGCCGGgtt                                                           >  4:242067/1‑151 (MQ=255)
                                                                                            gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑G‑A‑TT‑T‑GGGGCCAGGGGGGGTTTTTTTTTTACCAAGGAGAAGGGCAACAGCTGATTGCCCCTCACCCCCCGGCCCCGAGAGAGTTGCAGC‑AAGCGGGCCACGCTGGgtt                                                           >  1:140047/1‑150 (MQ=255)
                                                                                            gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGtt                                                            <  2:279009/149‑1 (MQ=255)
                                                                                               ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑G‑T‑TT‑T‑GGGGCCCGGGGGGGTTTTCTTTTTACCCGGGAGACGGGGCACAGCTGGTTTCCCCTCACCCCCCGGCCCTGGGAGAGTTGCAGC‑CAGCGGGCCCCCCCGGTTTGccc                                                       >  2:7352/1‑151 (MQ=255)
                                                                                                 aaTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGccca                                                      <  2:187521/150‑2 (MQ=255)
                                                                                                 aaTGAATCGGCCAACGCGCGGGGAGAGGCGGATTTG‑G‑T‑G‑CT‑GTGGCG‑CAGGGCGGGTTTTTTTTTCAACAAAGAGACGGGCAACAGCCGATTGCCCTTCACCGCCCGGGCCTGAGAGAGCTGTAGC‑AAACGGCCCACGCCGGTTTTccccc                                                     >  3:67172/1‑150 (MQ=255)
                                                                                                  aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCCGGGGGGGTTTTCTTTTTACCCGTGGGACGGGGAAAAGCCGATTGGCCTTCACCCCCCGGCCCTGAGAGAGGTGCAGC‑AAGCGGGCCCCCCCGGGTTTcccc                                                      >  2:1344626/1‑149 (MQ=255)
                                                                                                  aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCcag                                                    <  4:159569/151‑1 (MQ=255)
                                                                                                     aaTCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑G‑T‑T‑TT‑T‑GGCGCCAGGGGGGGTTTTTTTTTTTCCAGTGAGACGGGCCACAGCTGATTGCCCCTCACCCCCTGGCCCCCGGGGAGTTGCCGC‑CAACGGTCCCCCCCGGTTTTccccc                                                     >  2:795965/1‑146 (MQ=255)
                                                                                                     aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCCGGGGGGGTTTTTTTTTCACCAATGGGAAGGGCAAAAGCTGATTGCCCCTCCCCGCCCGGCCCTGGGAGGGGTGCAGC‑AAACGGTCCACGCTGGTTTGGCCccgc                                                   >  2:105321/1‑149 (MQ=255)
                                                                                                       tCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑T‑GGCGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCCTCACCGCCTGGCCCCGAGAGGGGTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCCgg                                                >  4:29304/1‑150 (MQ=255)
                                                                                                        cGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCg                                              <  2:467054/151‑1 (MQ=255)
                                                                                                          gCCAACGCGCGGGGAGAGGCGGATTTC‑G‑G‑T‑TT‑T‑GGCGCCGGGGGGGGTTTTCCTTTTTCCAGTTAGAAAGGCAACCACTGATTGCCCTTCACCCCCTGGCCCTGAGAGGGTTGTAGC‑AAGCGGGCCACGCTGGGTTGCCCCAGCAGGc                                               >  4:613900/1‑148 (MQ=255)
                                                                                                           ccAACGCGCGGGGAGAGGCGGGTTTG‑G‑T‑A‑TT‑G‑GGCGCCCGGGTGGGTTTTTTTTTTTCCAGTGTGACCGGGAACAGCTGGTTTTCCTTCACCCCCCGGCCCTTAAAGAGATGCAGCAAAGCGG‑CCACCCTGGTTTTCCCCAGCAgg                                                >  4:408202/1‑146 (MQ=255)
                                                                                                             aaCGCGCGGGGAGAGGCGGTTTTG‑G‑TGT‑TT‑G‑GGGGCCGGGGGGGTTTTT‑TTTTTACCCGGGGGAAGGGGAACAGCTGATTGTCCCTCACCGCCTGGCCCTGAGAGGGGTGGAGC‑AAGCGGGCCCCGCTGGGTTTCCCCAGCAGGGGgaaa                                          >  2:1149069/1‑150 (MQ=255)
                                                                                                               cgcgcgGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATcc                                       <  2:1250620/151‑1 (MQ=255)
                                                                                                                gcgcgGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCt                                      <  4:314842/151‑1 (MQ=255)
                                                                                                                  gcgGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTg                                     <  1:316766/150‑1 (MQ=255)
                                                                                                                   cgGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGtt                                   <  4:249745/151‑1 (MQ=255)
                                                                                                                     gggAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTg                                 <  3:193705/151‑1 (MQ=255)
                                                                                                                      ggAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGa                                <  2:1171108/151‑1 (MQ=255)
                                                                                                                               gTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                        >  1:149049/1‑150 (MQ=255)
                                                                                                                                tttGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTa                       >  2:761963/1‑150 (MQ=255)
                                                                                                                                 ttGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTaa                      <  2:21622/150‑1 (MQ=255)
                                                                                                                                  tGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAc                     <  3:447593/150‑1 (MQ=255)
                                                                                                                                     ag‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggc                  >  2:653072/2‑151 (MQ=255)
                                                                                                                                        t‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcgg                >  4:59748/1‑151 (MQ=255)
                                                                                                                                          a‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcgg                <  1:427243/150‑1 (MQ=255)
                                                                                                                                          a‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg               <  4:453213/151‑1 (MQ=255)
                                                                                                                                               g‑ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGat             >  4:902642/1‑150 (MQ=255)
                                                                                                                                                  gcgcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata          <  4:810677/151‑1 (MQ=255)
                                                                                                                                                   cgcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaa         <  2:145423/151‑1 (MQ=255)
                                                                                                                                                    gcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaa         >  1:406289/1‑150 (MQ=255)
                                                                                                                                                     ccAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaa         >  2:1379862/1‑149 (MQ=255)
                                                                                                                                                     ccAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAAc        <  4:1072632/150‑1 (MQ=255)
                                                                                                                                                       aGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg     <  1:114311/151‑1 (MQ=255)
                                                                                                                                                         ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaaaa       >  2:299677/1‑145 (MQ=255)
                                                                                                                                                         ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAt      >  4:320372/1‑148 (MQ=255)
                                                                                                                                                         ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg   <  2:1541634/151‑1 (MQ=255)
                                                                                                                                                          gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg   >  4:10669/1‑150 (MQ=255)
                                                                                                                                                          gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGc  <  3:140061/151‑1 (MQ=255)
                                                                                                                                                          gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGc  <  2:64911/151‑1 (MQ=255)
                                                                                                                                                           tggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg   >  2:530441/1‑149 (MQ=255)
                                                                                                                                                           |                                                                                                                                                      
AAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑G‑T‑A‑TT‑G‑GGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC‑AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366377‑366675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑