Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 514,624 | 0 | A | C | 40.6% | 174.2 / 11.6 | 96 | V79G (GTG→GGG) | ybbJ | inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX |
Reads supporting (aligned to +/- strand): ref base A (40/17); new base C (0/39); total (40/56) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.41e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAT > NC_000913/514475‑514774 | gaTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCAc < 2:1140352/151‑1 (MQ=255) aTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCAcc > 4:1653321/1‑151 (MQ=255) ccTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCAccc < 3:423483/150‑1 (MQ=255) ccTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCAccc < 4:1510135/150‑1 (MQ=255) ccTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCAccc < 4:912210/150‑1 (MQ=255) ccTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACccg > 2:841081/1‑151 (MQ=255) cGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGAACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACccgc < 4:167584/149‑1 (MQ=255) gCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCcgccgc > 1:186085/1‑151 (MQ=255) tGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAAcc > 2:1511608/1‑150 (MQ=255) ggCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTcc > 4:588885/1‑151 (MQ=255) aaCTGCCCCCGACGCGCATATGCCCGCCCCC‑GTTGCCCAGCGGAGATTCCAGCAAAAAACGCCGCCAAATCAGCTGCCCCCCGC‑GCTGTTTAAAATGACTATCGCTGTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACcaca < 4:186365/150‑1 (MQ=255) aCTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGc > 2:1534869/1‑151 (MQ=255) tGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGcc > 2:648069/1‑150 (MQ=255) gtcgcggcctcgtatatGACCGCCCCC‑TTTGACCTGGGAAGATTCCAGCACAAAACGGCGACAAACCGGCTGTCGCCGGC‑TTTGTTTAAATTAATTAGCGCTGTG‑TTTTGCTCGGCTCACCCGCCGCGACAACCATTTCCACCACAGCCa < 4:1247212/137‑1 (MQ=255) gccACCCACGCGCATATGCCCGCCCCC‑GTTGACCGGCGGAGATTCCAGCACAAACCGTCGGCCAATCAGCTGCTGCCCGC‑GTTGGTTAAAATGACTATCGCTGTGTTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCa < 2:1516848/148‑1 (MQ=255) ccgccgCGCATAGGCCCGCGCCC‑GTGGACCAGGGGAGTTCCCACCACAAACCGTCGCCCAATCAGCTGCCCCCCGC‑GTTGTTTTAAATGACTATCGCTGTGTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGcc < 2:1051914/145‑1 (MQ=255) gACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGgcggcg > 2:1053564/1‑151 (MQ=255) gcgcATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGAGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCTGCGagc > 2:544755/1‑151 (MQ=255) gcgcATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCGGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCACGACAACCCTTTCCACCACAGCCAGGCGGCGagc > 2:416511/1‑151 (MQ=255) cgcATATGCCCGCCCCC‑GTTGACCAGGGGAGATCCCAGCAAAAACCGCCGGCCAATCGCCTGCTGCCCGC‑GCTGGTTAAAATGACTACCGCTGTGTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGcc < 1:308993/139‑1 (MQ=255) gcATATGCCCGCGCCC‑GTTGACCAGCGGAGATTCCAGCAAAAAACGTCGGCCAATCAGCTGCTCCCCGC‑GTTGGTTAAAATGTCTATCGCTGTGTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCTACCACAGCCAGGCGGCGagc < 4:1320528/149‑1 (MQ=255) cgatatgccGCGCCCGGTTGACCGGGGGAGATTCCGGCAAAAACCGTGGCCAAAGCTCCTGCT‑CCCGCGGCTGTTTTAAATGCCTCTCGTTGTGCTTTTTTTC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagca < 2:1016377/143‑1 (MQ=255) atatGCCCGCGCCG‑GTGGACCGGCGGAGTTCCCAGCAAAAAACGCCGCCAAATCAGCTGCTGCCCGC‑GCTGTTTTAAATAACTACCGCTGTGTTTTTGTCC‑GCGCACCCGCCGCGACAACCATTTccacc < 2:1331805/130‑1 (MQ=255) atGCCCGCCCCC‑GTTGCCCGGGGGAGATCCCACCACAAACCGCCGGCAAATCAGCTGCCCCCCGC‑GCTGTTTAAAATGATTACCGCTTTTTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagca < 2:1339697/146‑1 (MQ=255) gcccGCGCCC‑GTTGCCCAGCGGAGTTTCCAGCAAAAAACGTCGGCCAATCAGCTGCTCCCCGC‑GCTGGTTAAAATGACTATCGCTGTGTTTTTGTCC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGt < 4:970897/146‑1 (MQ=255) ccGCGCCG‑TTGGCCCAGCGGGAATCCCAGCACAAAACGGCGCCCAATCGGCTGCCGCCCGC‑GCGGGTTTAAAGGCCTAGCCGGGTGTTTTTGTTC‑GCACCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGa < 2:1080572/151‑1 (MQ=255) ccGCGCCC‑GTTGACGGGGGGGGTTCCCAGCAAAAACCGGCGGCAAATCGGCTCCTGCCGGC‑GGTGGTTTAATTGCTTATCGCTTTGTTTTGGTCC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGt < 2:1213689/146‑1 (MQ=255) cgcgCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGAt > 3:38380/1‑151 (MQ=255) ccc‑TTTGACCAGGGGAGATTCCAGCAAAAAACGTGGGCAAATCAGCTGCTCCCCGC‑GCTGGTTTAAATGACTATCGTTGTCTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATgg < 4:746770/149‑1 (MQ=255) ccc‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGc > 1:174467/1‑150 (MQ=255) ttGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAAc > 4:1041895/1‑150 (MQ=255) ttGACCAGCGGAGATCCCAGCACAAACCGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTAAAATGACTTCCGCTGTTTTTTTGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAAc < 3:109498/150‑1 (MQ=255) ttGACCAGCGGAGATCCCAGCAAAAAACGCCGGCCAATCGGCTGCTGCCCGC‑GCTGTTTTAAATGCTTATCGCTGTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCaa < 2:1623407/148‑1 (MQ=255) gACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACAtt > 4:606919/1‑151 (MQ=255) ccAGCGGAGTTTCCAGCACAAACCGCCGGCCAATCAGCTGCTGCCCGC‑GCTGTTTTAAATGACTATCGCTGTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACAtt < 4:301965/149‑1 (MQ=255) ccAGCGGAGATCCCAGCAAAAACCGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGCCTATCGCTGTTTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTa < 4:1085263/150‑1 (MQ=255) ggggAGATCCCACAAAAAAGCGGCGGCCATCCAGTTGCGCCCCCC‑GCTGTTTTAAATGACTATCGTTGTGTTTTTGTTC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACa < 4:700317/142‑1 (MQ=255) gCGGAGTTCCCAGCAAAAAACGTCGGCCAATCAGCGGCTCCCCGC‑GGTGGTTAAAATGACTACCGCTGTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAccc < 4:39706/150‑1 (MQ=255) ggAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCtt > 4:1476534/1‑151 (MQ=255) ggAGATCCCAGCACAAACCGGCGGCAAATCGGCTGCGGCCCGC‑GCTGTTTTAAATGACTACCGCTGTTTTTTTGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCtt < 2:1685393/151‑1 (MQ=255) ggAAATCCCACCAAAAAGCGTGGGCCAATCAGCTCCGGCCCGC‑GTTGTTTAAAATGACTATCGCTGTGTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCt < 1:476572/150‑1 (MQ=255) gaagaTCCCACCACAAACCGCCGCCCAACCGCCTGCCGCCCGC‑GGTGTTTTAAAGACCTACCGCTGGTTTTTTTTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCt < 1:30055/148‑1 (MQ=255) gagaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTg > 4:1602509/1‑151 (MQ=255) agaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGc > 4:1364277/1‑151 (MQ=255) agaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGc > 1:102289/1‑151 (MQ=255) agaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGc > 2:459068/1‑151 (MQ=255) agaTTCCAGCAAAAACCGTCGCCCAACCCGCTGCTCCCCGC‑GTTGTTTAAAATGACTATCGCTGTGTTTTTGTCC‑GCCCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTg < 2:399133/150‑1 (MQ=255) agaTCCCAGCAAAAAACGCCGCCCAATCAGCTGCTGCCCGC‑GCTGTTTAAAATGACTATCGTTGTGTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTg < 3:174481/150‑1 (MQ=255) gttcccAGCAAAAACCGCGGCCCAACCAGCTGCTGCCCGC‑GTTGGTTTAAATGATTATCGCTGTTTTTTTTTTC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGcc < 4:726947/147‑1 (MQ=255) gaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGcc > 2:1641276/1‑151 (MQ=255) ccAGCAAAAACCGCCGGCCAATCAGCTGCTCCCCGC‑GTTGTTTTAAATGACTATCGCTGTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCAc < 4:717540/149‑1 (MQ=255) aGCACAAAGCGTCGCCCAACCGCCTGCTCCCCGC‑GCTGTTTAAAATGATTACCGCTGTTTTTTTGTGC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTccc < 1:238916/151‑1 (MQ=255) aGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTccc > 2:272632/1‑151 (MQ=255) caaaaacggccgcccAACCAGCTGCTCCCCGC‑GCGGGTTTAAATGCCTATCGCTTGTTTTTTGTTC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTccc < 2:1107079/136‑1 (MQ=255) aaacCGCCGGCAAATCCGCTGCTCCCCGC‑GCTGTTTAAAATCACTATCGCTGTGTTTTTGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTcca < 4:268870/146‑2 (MQ=255) aaacCGCCGGCAAATCAGCTGCTCCCCGC‑GTTGTTTTAAATGCCTCTCGCTGTGTTTTTGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAAcc < 4:1466703/150‑1 (MQ=255) aaaaCGTCGGCCAATCAGCTGCTGCCCGC‑GCTGTTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAAcc < 3:403010/150‑1 (MQ=255) aaCGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAAccc > 1:247727/1‑149 (MQ=255) aCGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGc > 3:389348/1‑151 (MQ=255) cGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCg > 3:69250/1‑151 (MQ=255) gTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCgg > 1:148229/1‑151 (MQ=255) tCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGc > 1:27455/1‑151 (MQ=255) ggCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAc > 3:331183/1‑151 (MQ=255) ggCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAc > 2:1273619/1‑151 (MQ=255) gCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAcc > 4:140358/1‑151 (MQ=255) ccAATCCGCTCCCCCCCGC‑GTTGTTTAAAAGGCCGTCCGCTGTCTTTTGGTCC‑GCCCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAcca < 2:191305/151‑1 (MQ=255) cAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAcc > 3:231768/1‑149 (MQ=255) cAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAcc > 4:1359559/1‑149 (MQ=255) ctcccggc‑gcTGTTTTAATTGACTCTCGCTGTGTTTTTGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGacc < 4:265419/142‑1 (MQ=255) cccGC‑GCTGTTTTAAATGCCTACCGTTGTGTTTTTTTCC‑CCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGaccac < 4:979428/147‑1 (MQ=255) cccGC‑GCTGGTTTAAATGACTATCGCTTTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAg < 3:216081/150‑1 (MQ=255) cccgcctttttttAAATGACTACCGCTGTCTTTTTTTGC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAccagcca < 4:345611/131‑1 (MQ=255) ccGC‑GTTGTTTAAAATTCCTACCGCTGTGCTTTTGCCC‑CCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGc < 2:1002865/151‑1 (MQ=255) ccgcgtttttttAAATGTTTTTCGCTGTTTTTTTGTTC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGc < 4:1633034/142‑1 (MQ=255) c‑gcTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCgg > 2:32149/1‑151 (MQ=255) cTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGt > 2:1604343/1‑150 (MQ=255) tGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTa > 2:1140785/1‑150 (MQ=255) tAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCAcc > 2:1416109/1‑151 (MQ=255) aaaTGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCg > 2:1412835/1‑151 (MQ=255) aatcccTATCGCTTTGTTTTTGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGc < 4:356768/146‑1 (MQ=255) atcaatATCGCTTTCGTTTCGTGC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGGGCAGCGTCAGGATGGCAAACATTACACCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCt < 2:514618/146‑1 (MQ=255) aTGACTGTCGCGGTGTTTTTGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCt < 2:1283778/151‑1 (MQ=255) tCGCTGTGTTTTTTTCC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcg < 3:244542/150‑1 (MQ=255) cgttttgtttttGTCC‑CCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcg < 4:920849/141‑1 (MQ=255) cggtgtttttttGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAc < 4:1056789/140‑1 (MQ=255) gCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgccg > 2:924438/1‑151 (MQ=255) tgtttttGTCC‑GCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcg < 3:472109/141‑1 (MQ=255) tttttGTGC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgc < 1:294564/142‑1 (MQ=255) ttttttCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCcac < 3:246826/150‑1 (MQ=255) ttttGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgccg < 1:377358/144‑1 (MQ=255) ttttGTCC‑GCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgcc < 4:423211/143‑1 (MQ=255) tttGTCC‑GCCCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCcaca < 2:1326918/150‑1 (MQ=255) tGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCacaac > 3:328551/1‑150 (MQ=255) c‑gcgCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAAt > 4:1357045/1‑150 (MQ=255) gcgcACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATa > 4:1661439/1‑150 (MQ=255) cgcCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACaaa < 4:459713/147‑1 (MQ=255) aCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAt > 4:1044730/1‑151 (MQ=255) aCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAt > 2:1011877/1‑151 (MQ=255) aCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAt > 1:423416/1‑151 (MQ=255) aCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAt > 1:404117/1‑151 (MQ=255) | GATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCC‑GTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGC‑GCTGGTTTAAATGACTATCGCTGTGCTTTTGTTC‑GCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCCACAACAAATAACCAT > NC_000913/514475‑514774 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |