Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC41.8% 184.1 / 21.2 98T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (46/11);  new base C (0/41);  total (46/52)
Fisher's exact test for biased strand distribution p-value = 8.76e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGG  >  NC_000913/1376356‑1376655
                                                                                                                                                        |                                                                                                                                                      
gctcgccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAaca                                                                                                                                                        <  2:456620/145‑1 (MQ=255)
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAacac                                                                                                                                                       >  4:1583995/1‑151 (MQ=255)
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAacac                                                                                                                                                       >  4:106632/1‑151 (MQ=255)
   tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                    >  2:512615/1‑151 (MQ=255)
    aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                    <  4:1384861/150‑1 (MQ=255)
    aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                                   <  3:166333/151‑1 (MQ=255)
     ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACAcc                                                                                                                                                      <  2:1403119/147‑1 (MQ=255)
     ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTca                                                                                                                                                  <  2:1281425/151‑2 (MQ=255)
       gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                                <  2:1354119/151‑1 (MQ=255)
         cgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGa                                                                                                                                              <  4:993069/151‑1 (MQ=255)
           aTGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                             <  2:1672766/150‑1 (MQ=255)
            tGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGc                                                                                                                                           >  4:1673665/1‑151 (MQ=255)
            acgctcttccgatctgCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGc                                                                                                                                           >  2:1467651/16‑151 (MQ=255)
             gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGc                                                                                                                                           <  4:401992/150‑1 (MQ=255)
                   gccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                                    >  4:1536185/1‑151 (MQ=255)
                     cgcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGtt                                                                                                                                   >  4:1000019/1‑150 (MQ=255)
                            ccTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcgtc                                                                                                                           >  1:12725/1‑151 (MQ=255)
                             cTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcgtcg                                                                                                                          >  3:212784/1‑151 (MQ=255)
                               ggAGGGCGCCCGAAAAAGGGGGAGTTTTCCCCTGCGCTTACGGGAACGGGTCCCCGCGCGCCCCGGAC‑CG‑GTGGTGTATCGCGACCCTTTAAC‑CCGTTATCAGGATCATATGACAACCCCCCTCGCCGATGCCCGCCGTTACAtcgt                                                                                                                            <  2:273206/147‑1 (MQ=255)
                               ggACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGa                                                                                                                         >  4:766618/1‑150 (MQ=255)
                                   ggCGCCCGAAAAAGGGTGAGTGTTCCCCGGCGCGTACGGAAACAGGCCGCCGCGCGTCCCGGAC‑CG‑GGGGTGTATCTCAACCCGTAAAC‑CCGCTATCAGGATAATAGGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                                    <  2:998722/151‑1 (MQ=255)
                                   ggCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                                    >  4:1314221/1‑151 (MQ=255)
                                   ggCGACCGAAAAATGGTGAGTGATCCCCTGCGCTTACGGGAACGGGTCGCCGCGCGTACCGGAC‑CC‑GTGGTTTTTCTCGAACCTTTAAC‑CCGCTATCAGGATAATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACg                                                                                                                    <  3:352022/151‑1 (MQ=255)
                                     cGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                                  >  2:117531/1‑151 (MQ=255)
                                     cGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                                  >  4:389940/1‑151 (MQ=255)
                                      gcccGAAAAATGGGGGGTGATTCCCGGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGGAA‑CG‑GTGTTTTTTCTCGAACGTTTAAA‑CCGCTATAAGGATCATATGATCACCCCCCGCGCCGAGGCCCGCCGTTACATCGTCGAAAACGa                                                                                                                   <  4:28941/147‑1 (MQ=255)
                                       aCCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                                >  1:379926/1‑151 (MQ=255)
                                        ccGAAAAATGGTGGGGGTTCCCGTGCGCTTACGGGACGGGGTCCCCGCGCGTCCCGAAC‑CG‑GTGGTGTATCGCGAA‑CGTTAAACCCGATACCAGGATCATATGACAAACCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATc                                                                                                                 <  4:1243717/149‑1 (MQ=255)
                                            aaaaTGGTGAGTTTTCCCCGGCGCGTACTGAAACAGGCCCCCGCGCGTCCCGGAC‑CG‑GGGGTTTATC‑CCAACCCTTAAACCCGCTACCAGGATCATATAACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                             <  2:632749/149‑1 (MQ=255)
                                            aaaaTGGTGAGTTATTCCCTGCGCGTCCGGAAACAGTCCGCCGCGCCTACCGGAC‑CC‑GGGGTTTATCTCGACCCGTTACC‑CCGCTATCAGGATCAAATAACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           <  2:912597/151‑1 (MQ=255)
                                            aaaaTGGTGAGTGATTCCCTGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGGAC‑CC‑GTGGTTTATCTCGACCCTTTAAA‑CCGCTATAAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                            <  4:241469/150‑1 (MQ=255)
                                            aaaaGGGGGAGTGTTCCCCTGCGCTTACGGGACCAGGTCGCCCCGCTTCCCGGAA‑CC‑GTGTTGTATCTCGAACATTAAAA‑CCCCTATAAGGATAAAATAATCACCCCCCTCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           <  3:92970/151‑1 (MQ=255)
                                                 ggggagtttttCCCTGGGCGTACGGGAACAGGTCGCCGCGCGTCCCGAAC‑CG‑GTGGTGTATCGCGACCCGTTAAC‑CCGTTACCAGGATAATAAGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                         <  4:1402464/139‑1 (MQ=255)
                                                  gtgagtgaTTCCCGGCGCGTACTGAAACGGGTCCCCGCGCGTCCCGGAC‑CG‑GTGGTTTATCTCGACCCGTTAAC‑CCGCTACAAGAATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                                        <  2:453757/148‑1 (MQ=255)
                                                  gggagtgtcccccGGCGCGTACTGAAACGGGTCGCCGCGCGCACCGGAC‑CC‑GTGTTGTTTCTCG‑ACCTTTAAACCCGCTATCAGGAAAATTGGATCAACCCCCTCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                     <  3:404371/141‑1 (MQ=255)
                                                    gagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                     >  4:1291335/1‑149 (MQ=255)
                                                       tgtttCCCTGCGCTTACTGGAACGGGTCGCCGCGCGTACCGGAC‑CG‑GTGGTGTATCTCGAACCGTAAAC‑CCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                                 <  4:744524/147‑1 (MQ=255)
                                                       tgttccccGGCGCGTACTGAAAAGGGCCGCCGCCCGTACGGAAA‑CC‑GTGGTTTATCGC‑AACCCTTAAACCCTATATCAGAATAAATTGACCACCCCCCACGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                                    <  2:1447374/142‑1 (MQ=255)
                                                       tgaTTCCCTGCGCGTACGGGAACGGGTCGCCGCGCGTACCGGAC‑CG‑GTGGTTTATCTCAACCCGTTAAC‑CCGCTATCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                                   <  4:882519/148‑1 (MQ=255)
                                                         aTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                              >  2:1212503/1‑151 (MQ=255)
                                                          tccccTGCGCGTACGGGAACAGGCCCCGGCGCGT‑CCGGAACCC‑GTGGTTTACCTCAAACCGTAAAC‑CCGTTATAAGGATCATATAAACACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                                 <  3:246668/145‑1 (MQ=255)
                                                            cccGGCGCGTACGGAAACGGGTCGCCGCGCGTCCCGGAC‑CG‑GTGGTTTATCTCAACCCGTAAAC‑CCGCTATCAGGATAATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                           <  4:1455157/151‑1 (MQ=255)
                                                             ccTGCGCGTGCTGAAACGGGTCGCCGCGCGTACGGGAA‑CC‑GTGGTGTATCCCGAACCTTAAAC‑CCGCTATCAGGATCATATGATCAACCCCCTCGCCGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGc                                                                                          <  4:1583673/151‑1 (MQ=255)
                                                                    gTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                                   >  4:1235783/1‑151 (MQ=255)
                                                                    gTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                                   >  4:822187/1‑151 (MQ=255)
                                                                      aCTGGAACAGGCCGCCGCGCGT‑CCGGAA‑CCGGGGGTGTATCGCGACCCGTAAAC‑CCGTTATAAGGTTAATATGATCAACCCCCTCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAttt                                                                                     <  1:444141/147‑1 (MQ=255)
                                                                         ggAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                              >  2:1603610/1‑151 (MQ=255)
                                                                               ggTCGCCGCGCGTCCCGGAC‑CC‑GTGGTGTATCTCGACCCGTAAAC‑CCGTTACAAGGATCATATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                                        <  4:1640464/151‑1 (MQ=255)
                                                                                   gccgcGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                    >  3:422671/1‑151 (MQ=255)
                                                                                   gccgcGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                    >  4:902239/1‑151 (MQ=255)
                                                                                    ccgcGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                    >  2:95711/1‑150 (MQ=255)
                                                                                        gcgtccggaaa‑CG‑GTGGTGTATCTC‑AACCCTTAAACCCGCTATCAGGATCATATGATAACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                                <  4:171363/141‑1 (MQ=255)
                                                                                        gcgTGCCGGAC‑CC‑GGGGTGTATCTCGAACCTTTAAA‑CCGTTAGCAGGATAATATAATCAACACCCTCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                               <  1:110149/151‑1 (MQ=255)
                                                                                         cgcaCCGGAA‑CC‑GTGGTTTATCTCGACCCGTTAAC‑CCGCTATAAGGATCATATGATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                               <  4:695294/147‑1 (MQ=255)
                                                                                         cgTACCGAAC‑CC‑GGGGGGTATC‑CCAACCCTTAAACCCGCTATCAGAATAATATAATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                                 <  2:1389552/148‑1 (MQ=255)
                                                                                          gTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                                >  4:249997/1‑148 (MQ=255)
                                                                                           tACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                                >  3:282798/1‑147 (MQ=255)
                                                                                             ccGGAC‑CC‑GTGGTGTATCTCGACCCGTTAAC‑CCGCTATCAGGATCATATGATCAACACCCTCACCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                               <  3:465819/146‑1 (MQ=255)
                                                                                             ccGGAA‑CC‑GTGTTGTATCTCGAACCGTAAAC‑CCGTTATCAGGATAATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                            <  3:269732/149‑1 (MQ=255)
                                                                                               ggAA‑CG‑GTGGTGTCTCTCGACCCTTAAAC‑CCGCTACCAGGATAATATAATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATa                                                           <  2:1434003/148‑1 (MQ=255)
                                                                                               ggAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                        >  2:358661/1‑151 (MQ=255)
                                                                                               cgggaccgggggtgTATCTCGACCGGTAAAC‑CCGCTATCGGGATCATATAATAACCCCCCGCGCTGTTTCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                       <  3:285666/128‑1 (MQ=255)
                                                                                                gAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                       >  3:442939/1‑151 (MQ=255)
                                                                                                  accc‑GTGGTGTATCCCAAACCTTAAAC‑CCGCTATCAGGATAATATGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                       <  2:1543627/148‑1 (MQ=255)
                                                                                                    cc‑GTGGTTTACCCCGACCCGTAAAC‑CCCCAATCAGAATCATAGGACAACCCCCCCCGCGGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAcc                                                        <  2:1671637/147‑1 (MQ=255)
                                                                                                    cc‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgc                                                    >  3:420470/1‑151 (MQ=255)
                                                                                                     c‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcg                                                   >  3:297080/1‑151 (MQ=255)
                                                                                                     c‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcg                                                   >  4:92921/1‑151 (MQ=255)
                                                                                                        tggtgTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                                  >  3:232514/1‑150 (MQ=255)
                                                                                                           tgtATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                              >  4:892369/1‑151 (MQ=255)
                                                                                                           tgtATCCCGAAGCTTAAAA‑CCGTTAGCAGAATAATATGATAACCCCCCCCCCCAATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAgg                                               <  1:9949/150‑1 (MQ=255)
                                                                                                            gtATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                             >  2:1324602/1‑151 (MQ=255)
                                                                                                             tATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcgc                                            >  3:2958/1‑151 (MQ=255)
                                                                                                               tctcGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                          >  2:4477/1‑151 (MQ=255)
                                                                                                               tctcGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                          >  4:594671/1‑151 (MQ=255)
                                                                                                                ctcGAACCGTTAAC‑CCGCTATCAGGATAATATGACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                              <  4:320326/146‑1 (MQ=255)
                                                                                                                  cGACCCGTAAAC‑CCGATAACAGGATAATATAATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCa                                        <  2:1234228/150‑1 (MQ=255)
                                                                                                                   gAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                                          >  3:167445/1‑115 (MQ=255)
                                                                                                                    acccGTTAAC‑CCGCTACCAGAATCATATAAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                                             <  4:1410291/141‑1 (MQ=255)
                                                                                                                    aaCCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                      >  3:334141/1‑150 (MQ=255)
                                                                                                                       ccttAAACCCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                          <  4:622134/142‑1 (MQ=255)
                                                                                                                           aaa‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                              >  4:971735/1‑151 (MQ=255)
                                                                                                                            aaccctttATCAGTATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAcc                                <  4:551952/142‑1 (MQ=255)
                                                                                                                                     tCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                     >  4:1568699/1‑151 (MQ=255)
                                                                                                                                        gattCATATGACAACCCCCCCCGCCGTGCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                     <  1:238366/145‑1 (MQ=255)
                                                                                                                                        gaaTCATATAACCAACCCCCTCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                     <  4:95873/146‑1 (MQ=255)
                                                                                                                                         gATAATATAATCAACCCCCTCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCgg                   <  1:252472/149‑1 (MQ=255)
                                                                                                                                          ataatatGATCTACACCCTCGCCGATGCCCGCCGTTACATCTTCGAGAACGATCTTAACCATGTACAGATTATCGGCGATTGCTATCACATGAATAACGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCCCGGTc                 >  2:300254/4‑150 (MQ=255)
                                                                                                                                             atagGACCAACCCCCTCGCCTATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCa                <  2:1395606/148‑1 (MQ=255)
                                                                                                                                              tatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGTGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg            >  2:1272145/1‑151 (MQ=255)
                                                                                                                                              tatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg            >  2:812740/1‑151 (MQ=255)
                                                                                                                                               atGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc           >  4:640098/1‑151 (MQ=255)
                                                                                                                                               atGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCa                <  2:1173254/146‑1 (MQ=255)
                                                                                                                                                  aTCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat         >  1:347177/1‑150 (MQ=255)
                                                                                                                                                   tCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat       >  3:137012/1‑151 (MQ=255)
                                                                                                                                                    cAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt      >  4:1610541/1‑151 (MQ=255)
                                                                                                                                                     aaCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt      <  2:821099/150‑1 (MQ=255)
                                                                                                                                                     aaCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt      <  3:342101/150‑1 (MQ=255)
                                                                                                                                                       cacCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCg   >  4:1154519/1‑151 (MQ=255)
                                                                                                                                                        acCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCgg  >  3:78239/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAA‑CC‑GTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGG  >  NC_000913/1376356‑1376655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑