Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,7480CA42.7% 176.9 / 72.1 118E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (54/13);  new base A (0/50);  total (54/64)
Fisher's exact test for biased strand distribution p-value = 4.90e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTT  >  NC_000913/3177603‑3177895
                                                                                                                                                  |                                                                                                                                                   
tCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGTTCAAAGGGT‑AGCAAGACTTCTTCGTGACCGCGCTCaaaa                                                                                                                                                 >  2:1276862/1‑150 (MQ=255)
  aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                              >  3:313066/1‑151 (MQ=255)
  aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAAtt                                                                                                                                              >  1:440679/1‑151 (MQ=255)
         tCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                       >  2:313296/1‑151 (MQ=255)
          cAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                       <  3:45603/150‑1 (MQ=255)
          cAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCccgcc                                                                                                                                       <  4:1191734/150‑1 (MQ=255)
                  ccGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGCCAAAGGGT‑AGCAAGACTGCGGCTTGCCCGCGTTCAAAAATTTCCCGCCGCACCTCAt                                                                                                                              <  1:339769/151‑1 (MQ=255)
                    ggCACCCATCCCCAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCGGCTCAATCAGCACACCTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACGGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATg                                                                                                                             <  2:876960/150‑1 (MQ=255)
                     gCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAc                                                                                                                           >  3:185244/1‑151 (MQ=255)
                     gCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAc                                                                                                                           >  3:255465/1‑151 (MQ=255)
                     gCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGAc                                                                                                                           >  4:1485151/1‑151 (MQ=255)
                       aaCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTc                                                                                                                         >  4:686241/1‑151 (MQ=255)
                        aCCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTc                                                                                                                         >  2:1668240/1‑150 (MQ=255)
                         ccATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAt                                                                                                                       >  2:225443/1‑151 (MQ=255)
                          cATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAtt                                                                                                                      >  4:1542946/1‑151 (MQ=255)
                           aTCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAttt                                                                                                                     >  4:1467011/1‑151 (MQ=255)
                           aTCCCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAACCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCAt                                                                                                                       <  4:446825/149‑1 (MQ=255)
                             ccccAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCAtt                                                                                                                      <  4:677231/146‑1 (MQ=255)
                                cAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                                >  4:731386/1‑151 (MQ=255)
                                 aGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAACCAGCACAACTTCACCCCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                                <  2:165530/150‑1 (MQ=255)
                                  gTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt                                                                                                              <  4:1451019/151‑1 (MQ=255)
                                    aGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                             >  2:1344011/1‑150 (MQ=255)
                                     gCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGa                                                                                                            >  3:151335/1‑150 (MQ=255)
                                      ccAAGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCAAAACTTCACCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                                <  4:689395/145‑1 (MQ=255)
                                         aGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  4:895365/1‑148 (MQ=255)
                                          ggggTTTCGCGGGTGCCGTACGCGGCAATCCGAATCTGCTCAATCAGCACACCTTCACCCCGCACGGGGTCAAAGGGT‑ACCAAGCCGGCGGCGTGACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                         <  1:362861/148‑1 (MQ=255)
                                               ttCGCTGGTGTCGTCCGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTAAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                  <  1:477664/150‑1 (MQ=255)
                                                 cGCTGGTGTCGTCCGCGGCAATCCGAATCTGCTCAACCAGCACAACTTCACCACGCACTGGGTCAAAGGGT‑AGCAAGCCGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                               <  4:642919/151‑1 (MQ=255)
                                                 cGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                               >  2:1007060/1‑151 (MQ=255)
                                                   cTGGTGTCGTCCGCGGCAACCCGAATCTGCTCAACCAGCACAACTTCATCCCGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                                <  2:472929/148‑1 (MQ=255)
                                                   cTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGa                                                                                              >  4:1438422/1‑150 (MQ=255)
                                                     ggTGTCGTGCGCGGAACCCCAAATCTCCCAAATCAGCACACCTTCATCACGCACTGGGAAAAAGGGT‑AGCAGGCCGGCGGGGTGACCGCGCAAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAAt                                                                                           <  2:659279/151‑1 (MQ=255)
                                                     ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACACCTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaaa                                                                                            <  4:614617/150‑1 (MQ=255)
                                                     ggTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaa                                                                                             <  3:450182/149‑1 (MQ=255)
                                                        gtcgtcCGCGGCAACCCGAATCTGCTCAATCAGCACACCTTCACCACCCCCTGGGTCAAAGGGT‑ACCAAGACTGCGTCGTCCCCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGaa                                                                                             <  2:1416508/146‑1 (MQ=255)
                                                        gtCGTACGCGGCAATCCAAACTTGCTCAATCCCCACAACTCCACCACGCCCTGGTTCAAGGGGT‑CGCAATTTTTCGCCTTATCCGGCCACAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGGCGa                                                                                              <  2:816427/145‑1 (MQ=255)
                                                            tACGCGGCAACCCGAATCTCCCAAATAAGCACACCTTCATCACGCTCTGGGTCAATGGGT‑ACAAAGACTGCGTCGGGACCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCt                                                                                         <  4:1477340/146‑1 (MQ=255)
                                                              cgcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                     >  2:1085089/1‑148 (MQ=255)
                                                                cgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACGCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                     >  2:349324/1‑146 (MQ=255)
                                                                 ggCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc                                                                                <  4:695941/150‑1 (MQ=255)
                                                                 ggCAACCCGAATCTGCTAAATCAGCACAACTTCATCACGCACGGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCt                                                                               <  4:870628/151‑1 (MQ=255)
                                                                  gCAATCCGATTCTGCCCATTCACCCCAACTTCATCACGCATTGGG‑CAAAGGGTGATCAAGACTGGGGGGTTCCCGCGCCCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaaa                                                                                    <  4:725755/145‑1 (MQ=255)
                                                                   cAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCCCTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGACTAGACGATGACGAAATCTATAAAGATCTa                                                                              >  2:384453/1‑150 (MQ=255)
                                                                   cAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              >  1:251409/1‑150 (MQ=255)
                                                                   cAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              >  2:831379/1‑150 (MQ=255)
                                                                    aaTCCGATTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑ACCAAGCCGGCGGCTTGCCCGTGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              <  1:338291/149‑1 (MQ=255)
                                                                    aaTCCGAATCTCCTCAACCAGCACACCTTCACCACGCACGGGGTCAAAGGGT‑AGCAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              <  2:676477/149‑1 (MQ=255)
                                                                     acccGAATCTGCTCAATCAGCACAACTCCACCACGCACTGGGTCAAAGGGT‑AACAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                           <  2:1274014/149‑1 (MQ=255)
                                                                     aTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                           >  1:244345/1‑151 (MQ=255)
                                                                     aTCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AACAAGACTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                           <  2:927871/151‑1 (MQ=255)
                                                                       ccaattcttctCAATAAGCCCAACTTCACCACGCACTGGGTAAAAGGGT‑AGCAAGCCTGCGGCGTGACCCCGCAAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGa                                                                          <  4:865323/145‑1 (MQ=255)
                                                                       ccGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGa                                                                          >  4:1125002/1‑150 (MQ=255)
                                                                       ccGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                         <  4:175395/151‑1 (MQ=255)
                                                                        cGAATCTGCTCAAACAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAGGACGGCGGCGTGACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              <  4:356170/145‑1 (MQ=255)
                                                                          aacctcctaaaTCACCCCAACTTCCCCCCGCACTGGGTCAAGGGGT‑ATCAAGTCTCCGCCTTACCCGCTCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaa                                                                       <  1:369630/141‑1 (MQ=255)
                                                                          aaTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaa                                                                       >  4:1246661/1‑150 (MQ=255)
                                                                          aaTCTGCTAAATCAGCACAACTCCATCACGCACGGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaa                                                                       <  2:1578788/150‑1 (MQ=255)
                                                                          aaTCTGCCAAATCACCACACCTTCACCACGCACTGGGTCAAAGGGT‑AGCAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                         <  2:143144/148‑1 (MQ=255)
                                                                           aTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGCTGACGCAATCTATAAAGATCTAATGaaa                                                                        >  1:476645/1‑148 (MQ=255)
                                                                            tCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaa                                                                     >  3:167090/1‑150 (MQ=255)
                                                                                cTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                                >  2:1132186/1‑151 (MQ=255)
                                                                                   aaTCAGCACACCTTCACCCCGCACGGGGTCAAAGGGT‑AGAAAGACTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGa                                                             <  2:44406/151‑1 (MQ=255)
                                                                                     ccAGCACAATTTCACCCCGCGTTGGGCAAAGGGGT‑AAAAAGACTGGGGCGTGCCCGCGCAAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATa                                                           <  2:1459948/150‑1 (MQ=255)
                                                                                      cAGCACACCTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGCCTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATa                                                           <  2:657302/150‑1 (MQ=255)
                                                                                      cAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATa                                                           >  3:65357/1‑150 (MQ=255)
                                                                                         cacaACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAg                                                        >  4:494650/1‑150 (MQ=255)
                                                                                           cagctccagcaCGCTCGGGGTAAAGGGTT‑ACCAAGACTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg                                                      <  1:27472/141‑1 (MQ=255)
                                                                                           caACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtgt                                                     >  4:894352/1‑151 (MQ=255)
                                                                                            accTTCATCCCGCACTGGGTAAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGtt                                                    <  3:244361/149‑1 (MQ=255)
                                                                                                 caccccgccgggggTCAAGGGGT‑AGAAAGACTGCGGCGGGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtct                                                 <  4:1008366/138‑1 (MQ=255)
                                                                                                  accaCGCACTGGGTAAAAGGGT‑AGAAAGACTGCGGCGTGCCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCg                                               <  2:1421212/148‑1 (MQ=255)
                                                                                                     aCGCCCGGGGTAAAAGGGA‑ACCAAGACGGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGc                                            <  4:811879/150‑1 (MQ=255)
                                                                                                       gCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         >  4:841041/1‑151 (MQ=255)
                                                                                                        cattGGGTCAAAGGTA‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         <  2:1253791/147‑1 (MQ=255)
                                                                                                        cACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         <  4:1487931/150‑1 (MQ=255)
                                                                                                        cACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         >  4:133974/1‑150 (MQ=255)
                                                                                                         aCTGGGTCAAAGGGA‑AGCAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaata                                        <  1:255450/150‑1 (MQ=255)
                                                                                                          cTGGGTCAAAGGGT‑AACAAGACTGCGGCGTAACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataa                                       <  1:149970/150‑1 (MQ=255)
                                                                                                            gggTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataat                                      <  4:1684233/149‑1 (MQ=255)
                                                                                                            gggTCAAAGGGT‑AGCAAGACGGCGGCGTGCCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAtt                                     <  4:212314/150‑1 (MQ=255)
                                                                                                                cAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTAc                                >  2:1486877/1‑151 (MQ=255)
                                                                                                                 aaggggT‑AGCAAGACGGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTa                                 <  2:1589030/146‑1 (MQ=255)
                                                                                                                  aaGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                              >  4:1579994/1‑151 (MQ=255)
                                                                                                                    gggT‑GGCAAGACTGCGGGGTGACCGCGCTAAAAATTTGCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                             <  1:267482/150‑1 (MQ=255)
                                                                                                                    gggT‑AGCAAGACTGCGGCGTGACCGCGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATc                             <  4:259254/150‑1 (MQ=255)
                                                                                                                         aGCAAGACTGCGGCGTGCCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtttt                        <  4:225807/151‑1 (MQ=255)
                                                                                                                          gCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGttttt                       >  2:1268441/1‑151 (MQ=255)
                                                                                                                             aGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGcc                    >  1:388523/1‑151 (MQ=255)
                                                                                                                                 tgcgcggtcaCCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAACGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTCCATCGttttt                       <  2:310222/135‑1 (MQ=255)
                                                                                                                                   cggcgTAACCCCGCTTAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAAt                <  4:1032582/149‑1 (MQ=255)
                                                                                                                                    ggcgTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGt              >  2:321737/1‑150 (MQ=255)
                                                                                                                                       gTGACCGCGCTCAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGt              <  2:1510084/147‑1 (MQ=255)
                                                                                                                                        tgcccGCGCCAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTaa            <  2:612194/145‑1 (MQ=255)
                                                                                                                                        tGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACgg         >  4:800587/1‑151 (MQ=255)
                                                                                                                                         gACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg        >  1:74395/1‑151 (MQ=255)
                                                                                                                                         gACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg        >  3:390716/1‑151 (MQ=255)
                                                                                                                                           ccGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg        <  4:661706/149‑1 (MQ=255)
                                                                                                                                           ccGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACgg         <  4:1138236/148‑1 (MQ=255)
                                                                                                                                              cgcTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGt   <  2:512102/151‑1 (MQ=255)
                                                                                                                                               gcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGtt  >  4:249739/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGT‑AGCAAGACTGCGGCGTGACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTT  >  NC_000913/3177603‑3177895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑