Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	43.8%	73.9 / 69.0	89	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/45);  new base T (39/0);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 4.08e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206287‑3206577                                                                                                                                                       |                                                                                                                                                 gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCa                                                                                                                                                <  4:1015132/151‑1 (MQ=255)     aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGAT‑TTTTTCAA‑Ac                                                                                                                                            >  4:1572928/1‑150 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACa                                                                                                                                     <  1:376066/151‑1 (MQ=255)               aCATTGCCATTCCCTGCTCTGGCGTAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACaaaaa                                                                                                                                 <  4:1452260/151‑1 (MQ=255)               aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGAA‑TTTTCTAG‑acaaaaacaaaa                                                                                                                                  >  4:1526792/1‑150 (MQ=255)                   tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTg                                                                                                                              <  2:470078/150‑1 (MQ=255)                   tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTTAA‑ACCAATAaaaaaaaagg                                                                                                                             >  4:95097/1‑147 (MQ=255)                    gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTTAG‑ACAaaaaaaaaaaatag                                                                                                                             >  2:1623472/1‑146 (MQ=255)                    gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGa                                                                                                                            <  2:115488/151‑1 (MQ=255)                      cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGt                                                                                                                          <  4:786320/151‑1 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGtt                                                                                                                         <  2:298240/151‑1 (MQ=255)                          cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCCG‑AAAAAAAAAAAAAATgg                                                                                                                             >  1:376943/1‑144 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCatga                                                                                                                  <  4:603138/151‑1 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCatg                                                                                                                   <  2:36053/150‑1 (MQ=255)                                tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCAG‑AAAAATAAAAAAACAAGAGTTGGCctga                                                                                                                  >  2:1669939/1‑149 (MQ=255)                                 ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                 <  4:742551/149‑1 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                              <  4:1644105/150‑1 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                              <  3:342944/150‑1 (MQ=255)                                    gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                            <  1:430784/151‑1 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAACAAAAAATGGAGTTGGCATGATGAGCg                                                                                                            >  4:375622/1‑149 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGaa                                                                                                          <  2:160659/151‑1 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCCG‑ACAAAAAAAAAAAATGGAGGTGGCATGATGAggg                                                                                                            >  4:1458588/1‑147 (MQ=255)                                        aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                        <  1:438599/151‑1 (MQ=255)                                          tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATATTTTCCA‑‑AAAAAAACACAAAATAGAGGTGGCCTGATGTGCGaa                                                                                                          >  2:1205969/1‑147 (MQ=255)                                           attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCAA‑ACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGaaaaaa                                                                                                      >  2:81377/1‑147 (MQ=255)                                             tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTTAT‑ACAAAAAAAAAAAATGGAGGTGCCCTGATGAGGGGAAGaaa                                                                                                     >  1:122670/1‑149 (MQ=255)                                                ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAAAAAAAAATGGAGTTGGCCTGATGGGGGAAAGtaataa                                                                                                  >  2:1167958/1‑149 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTTAA‑ACAAAAACAAAAAATAGAGGTGCCATGATGAGGGGAAGtaataaa                                                                                                 >  2:1037608/1‑148 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAAAAAAAACTGGAGGTGGCATGATGAGGGGAAAtaataa                                                                                                  >  4:547387/1‑148 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAA‑ACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGgaataa                                                                                                  >  4:1630672/1‑148 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCCG‑ACAAATACAAAAACTGGAGTTGCCATTATGAGCGGAAGgaataa                                                                                                  >  4:325928/1‑148 (MQ=255)                                                      tttcCGCGTTGATTCACGAAGTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCAA‑AAAAATAAAAAAAAACGAGTTTTCATGGTTTGCGAAAGAAAAAAACaaa                                                                                             >  2:555209/1‑147 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAATACAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACa                                                                                               >  1:448602/1‑145 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAACAAAAAATGGAGTTGGCATGATGAGGGAAAGGAATAAGCAACa                                                                                            >  3:282633/1‑148 (MQ=255)                                                         tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAAT‑TTTTTCA‑‑ACAAAAACAAAAAAATGAGGTGTCATGATGAGAGGAAGAAAAAAAAAACa                                                                                            >  1:60649/1‑146 (MQ=255)                                                             gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGAGGC‑GGGGAAA‑TTTTTTAA‑ACAAACAAAAAAAAAGGGGTTGGCATGTTTAGCGAAAGTAAAAAACaaaaagccgg                                                                                      >  2:759973/1‑140 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAA‑TTTTTCAA‑AAAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAGAAAAAAAAAAAAAGCAGTGaa                                                                                   >  1:101723/1‑149 (MQ=255)                                                                gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                <  2:1021772/151‑1 (MQ=255)                                                                    cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg                                                                            <  4:226159/151‑1 (MQ=255)                                                                    cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCAG‑ACAAAAAAAAAAAAAGGAGGTGTCATGATGAGGGGAAAGAAAAAACAAAAGGCAGAGAATaa                                                                                >  1:421871/1‑147 (MQ=255)                                                                     aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATTTAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAATAAAAAAAATGGAGTTGCCATGATGAGGGAAAGAAATAAACAACAAGCAGTGAATAAATTg                                                                            >  3:78200/1‑150 (MQ=255)                                                                      cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAAAAAAAAATGGAGTTTGCATGATGAGCGGAAGGAATAAACAAAAAGGAGGGAAAAAGtt                                                                             >  4:766982/1‑148 (MQ=255)                                                                        aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAA‑ACAAAAAAAAAAAATGGAGTTGCCATGATGAGAGAAAGAAATAAGAAACAGGCAGGGAATAAATTGACa                                                                         >  1:185831/1‑150 (MQ=255)                                                                         aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                       <  4:394014/151‑1 (MQ=255)                                                                         aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGATGACaga                                                                       <  2:94932/151‑1 (MQ=255)                                                                           tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAA‑ACAAAAAAAAAAAATGGAGGTGCCATGGTGGGCGAAAGTAATAAACAAAAGGAAGAGAAAAAAtt                                                                             >  2:485559/1‑143 (MQ=255)                                                                            gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCCG‑ACAAAAACAAAAAATGGAGGTGCCCAGATGAGGTAAAATAATAAAAAACAAGCAGGGAaaaaaattaca                                                                         >  4:1049581/1‑139 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GAtt                                                                  <  4:1025108/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GAtt                                                                  <  2:852436/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GAtt                                                                  <  4:567099/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACAATTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GAtt                                                                  <  2:1185029/150‑1 (MQ=255)                                                                                   aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGc                                                             <  1:407179/150‑1 (MQ=255)                                                                                   aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGc                                                             <  1:9808/150‑1 (MQ=255)                                                                                    cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAA‑ACAAATAAAAAAAATGGAGTTGCCCTGATGAGCGAAAGTAATAAGCAACAGGCAGGGAAAAAGTTGACAGA‑GATTTTCGCt                                                            >  3:214561/1‑150 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCCG‑ACAAAAACAAAAAATGGAGTTGCCCTGATGAGCGGAAGTAATAAACAACAAGCAGTGAATAAATTGACAGA‑GATTGTCGCt                                                            >  3:9187/1‑149 (MQ=255)                                                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCCG‑ACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGAAAATAATAAAAAAAAAGCAGTGAAAAAAATGACAGA‑AAtt                                                                  >  4:924088/1‑143 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAA‑ACAAATAACAAAACATGAGTTGTCACGTTGAGGGTAAGTAATAAGCAACAAGGCGGGAAAAAGTTGAAAGA‑GATTTTctc                                                             >  4:1155678/1‑144 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACt                                                        <  2:6148/151‑1 (MQ=255)                                                                                         tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACta                                                       <  2:1570983/150‑2 (MQ=255)                                                                                          cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑TTTTTCAG‑ACAAAAAAAAAAAAAGGAGTTTTCATGATGGGGGAAAGTAATAAAAAAAAGGGAGGGAATAAATTTAAAGA‑GATTGTGGCTAACttt                                                      >  1:180237/1‑150 (MQ=255)                                                                                           aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAACAAAAAATGGAGGTGCCATGGTGAGGGAAAAAAATAAACAACAGGGAGGGAATAAGTTGACAGA‑GATTGTCGCTAACttt                                                      >  4:572411/1‑149 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTAcc                                                   <  4:17530/151‑1 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTAcc                                                   <  2:332128/151‑1 (MQ=255)                                                                                               tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCCG‑ACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGAAATAAAAAACAGGGAGGGAATAAATTGACaga                                                                       >  4:1464793/1‑129 (MQ=255)                                                                                               tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAga‑ga                                                                    <  2:1462966/131‑1 (MQ=255)                                                                                                   aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAA‑ATTTTAAA‑ACAAATACAAAAAATGGAGGTAGCATTATTGGGGAAAAAAAAAAACAAAAAGGAGGTAAAAAGGAGACAAA‑GATTTTCACTAAATTTaaccgc                                                >  2:146465/1‑142 (MQ=255)                                                                                                    aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAAAAAAAACTGGAGTTGGCATGATGAGAGAAAGGAATAAACAAAAGGCAGTGGATAAATTGAAAGA‑GATTGTTGCTAAATTTACAGCCATg                                             >  4:490141/1‑149 (MQ=255)                                                                                                     aaaaTATAAACCCACATTTTTAGTGGTATTTTGTGGC‑GGTGAAT‑TTTTCCAG‑AAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGAtt                                          <  2:332122/151‑1 (MQ=255)                                                                                                     aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGAtt                                          <  1:383536/151‑1 (MQ=255)                                                                                                              accctcaTTGTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAA‑AAAAAAAAAAAAATTGGAGTTCCAATGCTGACCGAAAGTAATAAGCACCAGGCTTTGAAAATGTTGACAGA‑GATTGTGGTTAACTTTACCGCCATGATTTCTACCCGa                                 <  4:1493416/146‑1 (MQ=255)                                                                                                              aaCCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAATTTTACCGCCATGATTTCTACCCGa                                 <  2:137867/151‑1 (MQ=255)                                                                                                               aCCACATTTTTAGTGGTGGTTTGTGGC‑GGTGAAT‑TTTCCCAG‑ACAAATACAAAACCTGGAGTTGCCATGATGAGCAAAAGAAATAACAAACAGGCAGTGAATAAGTGGACAAA‑GATTGTCGCTAACTTTTCCGCCATGATTTCTACCCGaa                                <  4:1109726/151‑1 (MQ=255)                                                                                                                ccACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAAAAAAAAATGGAGTTGCCCTGATGAGCGAAAGAAATAAACAAAAAGCAGGGAAAAAAGTGACAGA‑GATTGTCGCTAAATTTTACGCCCTGATTTTTACCCGaa                                >  4:298462/1‑150 (MQ=255)                                                                                                                ccACATTTTTAGTGGTAGTTTGTGGC‑GGTAAAT‑TTTTCCAG‑AAAAATACAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                               <  4:1576429/151‑1 (MQ=255)                                                                                                                      ttttAGTGGTATTTTGTGGT‑GGTGATT‑TTTTCCAA‑AAAAATAAAAAACCGGGAGTTCCCATGATGAGCAAAATTAAAAAGCAACAGTCAGTAAATAGTTTGACAGA‑GATTCTCCTTCACTTTACCGCCATGATTTCTACCCGAATGCCta                          <  2:426200/150‑2 (MQ=255)                                                                                                                      ttttAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAG‑ACAAAAACAAAAAATGGAGTTGCCATGATGAGGGGAAGTAATAAACAACAGGCAGGGAAAAAATTGACAGA‑GATTTTCGCTAAATTTTCCCCCCTGATTTTTTCCCGAATGcc                            >  4:338673/1‑148 (MQ=255)                                                                                                                       tttAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATAATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtga                         <  2:458309/150‑1 (MQ=255)                                                                                                                         tAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTTCAGAAAAAAAAAAAAAACTGG‑GGTGTCATTGTGAGGGAAAAGAATAAGGAACAAGCAGGGAAAAAAGTTAAAGAGGATTTTGGCTAA‑TTTAACGCCATGATTTTTACCCGCATGcccggt                        >  2:265399/1‑145 (MQ=255)                                                                                                                           gTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatga                      <  4:1123593/149‑1 (MQ=255)                                                                                                                             ggTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                   <  2:490091/150‑1 (MQ=255)                                                                                                                              gTAGTTTGTGGC‑GTTAATT‑TTTTCCAA‑ACAAATAAAAAAACTGGAGTTGCAATGATGAGCAAAAGAAATAACCAACAGGCAGTGAATAAGTTGACAGA‑GATTTTCCCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                  <  2:546631/150‑1 (MQ=255)                                                                                                                              gTAGTTTGTGGC‑GGTGAAT‑TTTTTCCG‑ACAAATACAAAAAATGGAGTTGCCATGATGGGCGGAAGTAATAAACAAAAAGCAGGGAATAAGTTTACAGA‑GATTGTCGCTAAATTTTCCGCCCTGATTTCTAACCGAATACCTGATGACgtgg                 >  1:212997/1‑151 (MQ=255)                                                                                                                                 ttttGTGGG‑GGTAATT‑TTTCCCAG‑AAAAATAAAAAAACTGGGGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGGGAGA‑ATTTGTCGCAACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                    <  4:1155372/144‑1 (MQ=255)                                                                                                                                    tgtgGC‑GGTGAAT‑TTTTCCAG‑AAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt            <  4:321695/150‑1 (MQ=255)                                                                                                                                     ggggC‑GGTGATT‑TTTCCCAG‑AAAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAAAGA‑GTTTGTCGGTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt            <  2:413564/147‑1 (MQ=255)                                                                                                                                           ggTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGA‑GATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa      <  2:1257532/150‑1 (MQ=255)                                                                                                                                            gTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa     >  2:998613/1‑150 (MQ=255)                                                                                                                                            gTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa     >  4:1037165/1‑150 (MQ=255)                                                                                                                                               aaT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc  >  4:656795/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                 GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAT‑TTTTCCAG‑ACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGA‑GATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206287‑3206577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑