Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 25,559 A→G H118R (CAC→CGC)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091325,5590AG99.1% 408.5 / NA 113H118R (CAC→CGC) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (59/53);  minor base T (0/1);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 114 total aligned reads displayed.

TTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAAC  >  NC_000913/25413‑25706
                                                                                                                                                  |                                                                                                                                                   
ttGGTTTTTTGCCGGTATGGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGcgcg                                                                                                                                                   <  2:1290695/149‑1 (MQ=255)
    ttttttGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCGGTGATGATGTACCCCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTc                                                                                                                                              <  2:803906/147‑1 (MQ=255)
    ttCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCAAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTc                                                                                                                                              <  2:1428018/150‑1 (MQ=255)
      cTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGtt                                                                                                                                           >  2:954990/1‑151 (MQ=255)
        tggCCGGTATTGCGATGGGTATTAGCGTGATCCTGGCAGTGAGGATTTACCGCTCGAAGCCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTCCGACCGCCTGTGGCGCGGCTTCGtt                                                                                                                                           <  2:1487007/147‑1 (MQ=255)
           ccGTTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGTTGATTTTTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGt                                                                                                                                         <  2:1252138/148‑1 (MQ=255)
             ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCCCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGa                                                                                                                                      <  2:1583734/149‑1 (MQ=255)
             ggTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCg                                                                                                                                       >  2:509744/1‑148 (MQ=255)
                 ttGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGCGCGGCTTCGTTGTCGATATGATc                                                                                                                               >  3:353928/1‑151 (MQ=255)
                 ttGCGATTGGTATTAGCGTGATCCTGGAAGTGATGATGTATCGCTCGAAGGCCCCGCAGAAGCTAAACAATATCGCTTACGCGCTGTTTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGa                                                                                                                                 <  3:218032/150‑1 (MQ=255)
                 ttGCGATGGGTATTAGCTTGATCCTGGAAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGTTAAACAATATCGCTTCCGCGTTGTTTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTc                                                                                                                                        <  2:1627242/143‑1 (MQ=255)
                   gCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAGCTTGTTCGACCGCCTGTGGCTCGGCTTCGTTGTCGATATGATc                                                                                                                               <  3:387494/150‑1 (MQ=255)
                    cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCg                                                                                                                              >  4:1171651/1‑150 (MQ=255)
                     gATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGa                                                                                                                             <  3:196552/150‑1 (MQ=255)
                         ggTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGGGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACtt                                                                                                                          <  4:1015728/149‑1 (MQ=255)
                           tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGTTAAACAATATCGCTTACGCGCTGATTTTTGGCGGGGCGCGGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                                       <  2:1052636/150‑1 (MQ=255)
                           tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCAAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTTTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                                       <  2:514627/150‑1 (MQ=255)
                            aTTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGCCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTAc                                                                                                                      <  4:415394/150‑1 (MQ=255)
                                    gATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGAc                                                                                                             >  2:1616572/1‑151 (MQ=255)
                                     aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                                            <  3:361452/151‑1 (MQ=255)
                                     aTCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACt                                                                                                            >  2:1040027/1‑151 (MQ=255)
                                      tCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTg                                                                                                           >  4:946824/1‑151 (MQ=255)
                                       ccTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTg                                                                                                           <  3:257252/150‑1 (MQ=255)
                                       ccTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTgg                                                                                                          <  1:353911/150‑1 (MQ=255)
                                        cTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGc                                                                                                         >  4:884132/1‑151 (MQ=255)
                                          ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGc                                                                                                         <  2:254844/149‑1 (MQ=255)
                                          ggCAGTGATGATGTAGCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTCCGCGCTGATTATGGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCa                                                                                                        <  4:534472/150‑1 (MQ=255)
                                          ggCAGTGATGAGGTATCGCTGGAAGCCCACCCAGAAGTGAACCATTTTCGCTTACGCCCTGTTTATGGGCGGCGCGTTGGGCACCCTGTTGGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCa                                                                                                        <  2:967381/150‑1 (MQ=255)
                                             aGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTc                                                                                                    >  3:131973/1‑151 (MQ=255)
                                              gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTc                                                                                                    <  1:173470/150‑1 (MQ=255)
                                              gTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                                   >  4:593796/1‑151 (MQ=255)
                                                gatgatGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTc                                                                                                    <  3:158422/148‑1 (MQ=255)
                                                 atgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGcc                                                                                                 >  2:652029/1‑150 (MQ=255)
                                                     tgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCtt                                                                                            >  3:188985/1‑151 (MQ=255)
                                                     tgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCtt                                                                                            >  4:813895/1‑151 (MQ=255)
                                                          cGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTTTTGGCGGCGCGTTGGCCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCa                                                                                          <  2:1073232/148‑1 (MQ=255)
                                                               gAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGcc                                                                                  >  2:902877/1‑151 (MQ=255)
                                                                aaGGCCACGCAGAAGCAAAACAATATCGCTTACGGGCTGATTATTGGCGGCGCGCGGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGcct                                                                                 <  2:992360/151‑2 (MQ=255)
                                                                     cACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACt                                                                            >  4:1589559/1‑151 (MQ=255)
                                                                     cACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACt                                                                            >  4:854927/1‑151 (MQ=255)
                                                                      aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTg                                                                           >  4:1468351/1‑151 (MQ=255)
                                                                      aCGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTg                                                                           >  2:253229/1‑151 (MQ=255)
                                                                       cGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGc                                                                          >  2:1448350/1‑151 (MQ=255)
                                                                       cGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGc                                                                          >  2:1262946/1‑151 (MQ=255)
                                                                        gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                                         >  2:1272245/1‑151 (MQ=255)
                                                                        gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                                         >  2:307050/1‑151 (MQ=255)
                                                                          aGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCAt                                                                       >  2:1091708/1‑151 (MQ=255)
                                                                            aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCt                                                                     >  2:546752/1‑151 (MQ=255)
                                                                            aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCt                                                                     <  4:1533157/151‑1 (MQ=255)
                                                                              gCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                                   >  1:16987/1‑151 (MQ=255)
                                                                               cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                                  >  1:333943/1‑151 (MQ=255)
                                                                               cTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                                   <  4:515370/150‑1 (MQ=255)
                                                                                 aaaCAATTTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                                  <  4:461783/149‑1 (MQ=255)
                                                                                 aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTc                                                                <  3:424086/151‑1 (MQ=255)
                                                                                 aaaCAATACCGCTTCCGCGCTTATTATTGGCGGCCCGCGGGCCAACCTTTTCGCCCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTc                                                                <  2:1491074/151‑1 (MQ=255)
                                                                                  aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCg                                                               >  4:96058/1‑151 (MQ=255)
                                                                                    cAATATCGCTTAGGCGTTGATTATTGGCGGCGCGCGGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGt                                                             <  2:200514/151‑1 (MQ=255)
                                                                                     aaTATCGTTTACGCGCTGATTATTGGCGGCGCGCGGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGt                                                             <  2:1575686/150‑1 (MQ=255)
                                                                                     aaTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTg                                                            <  3:149356/151‑1 (MQ=255)
                                                                                          cGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGc                                                        >  3:448293/1‑150 (MQ=255)
                                                                                          cGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGc                                                        >  4:1497258/1‑150 (MQ=255)
                                                                                           gCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCAc                                                      >  4:206756/1‑151 (MQ=255)
                                                                                              tgcgcgCTGTTTATTGGCGGCGCGGGGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTg                                                    <  2:958014/148‑1 (MQ=255)
                                                                                              tACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                                    >  3:390113/1‑134 (MQ=255)
                                                                                              tACGCGCTGATTATGGGCGGCGCGCTGGGAAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                                    <  1:390096/134‑1 (MQ=255)
                                                                                              tACGCGCTAATTATTGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTg                                                    <  3:407517/150‑1 (MQ=255)
                                                                                               aCGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAt                                                  >  4:649512/1‑151 (MQ=255)
                                                                                                cgcgCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAtt                                                 >  2:1204678/1‑151 (MQ=255)
                                                                                                 gcgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAtt                                                 >  2:656942/1‑150 (MQ=255)
                                                                                                 gcgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtg                                                >  4:26465/1‑151 (MQ=255)
                                                                                                 gcgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtg                                                >  2:1013894/1‑151 (MQ=255)
                                                                                                  cgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                                               >  2:1352728/1‑151 (MQ=255)
                                                                                                   gcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                                              >  4:769664/1‑151 (MQ=255)
                                                                                                   gcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                                              >  3:93786/1‑151 (MQ=255)
                                                                                                      gATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                                           >  4:633911/1‑151 (MQ=255)
                                                                                                       atttttGGCGTCGCGCGGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                                           <  1:426356/150‑1 (MQ=255)
                                                                                                        ttttgggcgccGCGCGGGGCACCGTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                                              <  2:1587598/141‑1 (MQ=255)
                                                                                                          attGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGTTATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGACGGTGCGGCACTGGTTGTGGTGGaa                                        >  2:856959/1‑150 (MQ=255)
                                                                                                          attGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGa                                         >  4:185511/1‑149 (MQ=255)
                                                                                                          atgggcggcGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGaa                                        <  2:829726/147‑1 (MQ=255)
                                                                                                           ttGGCGGCGCGCTGGGCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGaa                                        <  1:331377/149‑1 (MQ=255)
                                                                                                            tGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                                     >  4:659761/1‑151 (MQ=255)
                                                                                                             ggcggcGCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtt                                    >  4:1284422/1‑151 (MQ=255)
                                                                                                               cggcgCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                                  >  2:866554/1‑151 (MQ=255)
                                                                                                               cggcgCGCTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                                  >  1:82365/1‑151 (MQ=255)
                                                                                                                ggcgCGGTGGTCACCCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                                  <  4:645360/150‑1 (MQ=255)
                                                                                                                   gcgcTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTg                               <  4:1291845/150‑1 (MQ=255)
                                                                                                                     gcTGGGCAACCTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCt                            >  2:1262328/1‑151 (MQ=255)
                                                                                                                            acccTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTag                       <  3:303596/147‑1 (MQ=255)
                                                                                                                            acccTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTag                       <  2:592975/147‑1 (MQ=255)
                                                                                                                                 gTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaa                 <  4:253634/150‑1 (MQ=255)
                                                                                                                                  ttCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaaa                >  4:1184592/1‑150 (MQ=255)
                                                                                                                                  ttCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGa                  <  4:510447/148‑1 (MQ=255)
                                                                                                                                    cGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGaaaaa              <  1:131959/150‑1 (MQ=255)
                                                                                                                                         gCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACaata        <  4:930878/151‑1 (MQ=255)
                                                                                                                                         gCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACaat         >  2:510492/1‑150 (MQ=255)
                                                                                                                                           cTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACaataat      >  2:1620094/1‑151 (MQ=255)
                                                                                                                                             gtgGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACaataat      <  1:188971/149‑1 (MQ=255)
                                                                                                                                             gtgGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAataata     <  4:147731/150‑1 (MQ=255)
                                                                                                                                               ggCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAAc  <  3:433855/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAAC  >  NC_000913/25413‑25706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: