Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,838,061 G→A C15C (TGC→TGT hydN ← formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,838,0610GA98.0% 385.4 / ‑4.4 100C15C (TGC→TGThydNformate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/60);  total (38/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCA  >  NC_000913/2837913‑2838203
                                                                                                                                                    |                                                                                                                                              
gTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACa                                                                                                                                              <  3:259713/151‑1 (MQ=255)
  gCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACaa                                                                                                                                             <  1:428774/150‑1 (MQ=255)
    aTCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAAcc                                                                                                                                           >  1:344869/1‑150 (MQ=255)
        tCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCaa                                                                                                                                         <  4:1144582/148‑1 (MQ=255)
        tCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCa                                                                                                                                          <  4:1021272/147‑1 (MQ=255)
        tCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAAt                                                                                                                                        <  1:463651/149‑1 (MQ=255)
        tCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATa                                                                                                                                       <  2:896989/150‑1 (MQ=255)
        tCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATAc                                                                                                                                      >  2:516914/1‑151 (MQ=255)
         cacaCTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATAc                                                                                                                                      >  3:361551/1‑150 (MQ=255)
            acTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACAttt                                                                                                                                  <  2:871250/151‑1 (MQ=255)
             cTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACAttt                                                                                                                                  <  2:1490174/150‑1 (MQ=255)
                   gCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTcgcg                                                                                                                           <  2:1484282/151‑1 (MQ=255)
                   gCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTcgcg                                                                                                                           >  4:1415556/1‑151 (MQ=255)
                   gCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTcgcg                                                                                                                           <  4:1673838/151‑1 (MQ=255)
                   gCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACAACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTcgcg                                                                                                                           <  3:102286/151‑1 (MQ=255)
                    cAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGt                                                                                                                          <  3:269186/151‑1 (MQ=255)
                    cAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGt                                                                                                                          >  1:412728/1‑151 (MQ=255)
                       aCTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAg                                                                                                                       <  4:1297279/151‑1 (MQ=255)
                         tGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGc                                                                                                                      >  4:1686139/1‑150 (MQ=255)
                          gTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCa                                                                                                                     <  2:731136/150‑1 (MQ=255)
                          gTCGACGTGGAAATGTTCACCCCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGCGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACGTCACAGGTGCGGCAACCAATACATTTACTCGCGTCATCaa                                                                                                                    <  2:654691/151‑1 (MQ=255)
                            cGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAatg                                                                                                                  <  3:309127/151‑1 (MQ=255)
                             gCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAatg                                                                                                                  >  2:417184/1‑150 (MQ=255)
                             gCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAatg                                                                                                                  >  2:1123408/1‑150 (MQ=255)
                             gCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAatg                                                                                                                  >  2:1218924/1‑150 (MQ=255)
                               cGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAatgatg                                                                                                               <  3:284875/151‑1 (MQ=255)
                               cGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAatgatg                                                                                                               <  2:624383/151‑1 (MQ=255)
                                  ggAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGaa                                                                                                             >  2:104552/1‑150 (MQ=255)
                                      aTGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt                                                                                                        <  2:983101/151‑1 (MQ=255)
                                         ttCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc                                                                                                      >  4:66376/1‑150 (MQ=255)
                                          tCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat                                                                                                    >  2:1093707/1‑151 (MQ=255)
                                             cacCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca                                                                                                  >  3:396751/1‑150 (MQ=255)
                                              acCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa                                                                                                <  2:899375/151‑1 (MQ=255)
                                               ccTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa                                                                                                <  3:80331/150‑1 (MQ=255)
                                                 tttAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc                                                                                             >  4:233625/1‑151 (MQ=255)
                                                   tAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct                                                                                            >  4:710681/1‑150 (MQ=255)
                                                       gACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAg                                                                                        <  2:169482/150‑1 (MQ=255)
                                                        aCATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCa                                                                                      <  2:667889/151‑1 (MQ=255)
                                                         cATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa                                                                                     <  2:716739/151‑1 (MQ=255)
                                                          aTGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt                                                                                    <  4:1309436/151‑1 (MQ=255)
                                                          aTGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt                                                                                    >  4:217561/1‑151 (MQ=255)
                                                            ggATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa                                                                                  <  2:1397399/151‑1 (MQ=255)
                                                               tACGCGGTAAAAAAGTTTCCGCGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTTACAGGTCCGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATACTCCAGCAATGACa                                                                                <  1:20605/150‑1 (MQ=255)
                                                                  gcgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTa                                                                            <  2:909546/151‑1 (MQ=255)
                                                                     gTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATg                                                                         >  3:443716/1‑151 (MQ=255)
                                                                     gTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATg                                                                         <  4:183394/151‑1 (MQ=255)
                                                                     gTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCATGATGAGAAACCACGCACGCTACTTCTTATTTTTGTCAACCAATACACCTACCCGCTTCACCAATCTTGAACCCCTTCCTCTACCTCTCCAGCACTTTCAGCTAAtc                                                                         <  2:1291575/151‑2 (MQ=255)
                                                                       aaaaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATgcg                                                                       <  3:443115/151‑1 (MQ=255)
                                                                          aaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCg                                                                    >  4:1309097/1‑151 (MQ=255)
                                                                             gTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa                                                                 <  1:13204/151‑1 (MQ=255)
                                                                               ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATAc                                                                >  4:956776/1‑150 (MQ=255)
                                                                               ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATAc                                                                >  4:648333/1‑150 (MQ=255)
                                                                               ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATAc                                                                >  3:368380/1‑150 (MQ=255)
                                                                               ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATAc                                                                >  2:366719/1‑150 (MQ=255)
                                                                               ttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATAc                                                                >  2:457811/1‑150 (MQ=255)
                                                                                tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAt                                                              >  4:434328/1‑151 (MQ=255)
                                                                                 ccGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt                                                             >  1:86803/1‑151 (MQ=255)
                                                                                 ccGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt                                                             >  2:723623/1‑151 (MQ=255)
                                                                                 ccGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt                                                             <  2:433724/151‑1 (MQ=255)
                                                                                   ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTca                                                           <  2:211711/151‑1 (MQ=255)
                                                                                      gTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACaa                                                        <  2:1360507/151‑1 (MQ=255)
                                                                                        cAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACaaa                                                       <  1:224557/150‑1 (MQ=255)
                                                                                        cAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACaaa                                                       <  2:851812/150‑1 (MQ=255)
                                                                                        cAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACaaa                                                       <  2:955525/150‑1 (MQ=255)
                                                                                        cAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAAt                                                      <  2:644457/151‑1 (MQ=255)
                                                                                        cAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAAt                                                      >  1:160532/1‑151 (MQ=255)
                                                                                         aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAAt                                                      >  2:1042042/1‑150 (MQ=255)
                                                                                           cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCAt                                                   <  4:1276357/151‑1 (MQ=255)
                                                                                           cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCAt                                                   >  1:398788/1‑151 (MQ=255)
                                                                                           cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCAt                                                   <  4:1672831/151‑1 (MQ=255)
                                                                                            gATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATg                                                  <  4:1009901/151‑1 (MQ=255)
                                                                                             aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATg                                                  <  2:709726/150‑1 (MQ=255)
                                                                                               gCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGcc                                                <  3:37645/150‑1 (MQ=255)
                                                                                                  caGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGtt                                            <  2:83958/151‑1 (MQ=255)
                                                                                                  caGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGt                                             <  4:1646769/150‑1 (MQ=255)
                                                                                                   aGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGttt                                           >  4:1670458/1‑151 (MQ=255)
                                                                                                      ccTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGttttt                                         <  2:1536137/150‑1 (MQ=255)
                                                                                                         gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAAtt                                     <  4:940948/151‑1 (MQ=255)
                                                                                                         gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAAtt                                     >  4:916809/1‑151 (MQ=255)
                                                                                                          aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAAttt                                    <  4:1219663/151‑1 (MQ=255)
                                                                                                          aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAAttt                                    >  4:1033841/1‑151 (MQ=255)
                                                                                                                  gatgaGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGTTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGtt                              <  1:116740/149‑1 (MQ=255)
                                                                                                                  gatgaGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGtt                              <  2:56930/149‑1 (MQ=255)
                                                                                                                  gatgaGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTAt                            >  3:303275/1‑151 (MQ=255)
                                                                                                                  gatgaGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTAt                            <  2:1684102/151‑1 (MQ=255)
                                                                                                                    tgaGAAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTAttt                          >  1:241994/1‑151 (MQ=255)
                                                                                                                     gagaAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTa                         <  4:571807/151‑1 (MQ=255)
                                                                                                                      agaAACCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTaa                        <  2:1345000/151‑1 (MQ=255)
                                                                                                                        aaaCCACGCACGCTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAgg                      <  2:665980/151‑1 (MQ=255)
                                                                                                                            gacgcacgcAACGTCGCGGGTGGGGAAAGGAATAGATTTACTCGAGTAATCCATGGTGCACCGGTTCATCAAAAAATAAAAAAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAaat                  >  2:793111/2‑151 (MQ=255)
                                                                                                                              cgcacgcTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAaatt                 >  2:1407946/1‑150 (MQ=255)
                                                                                                                                cacgcTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAaattaat              <  2:1539503/151‑1 (MQ=255)
                                                                                                                                   gcTACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTc           >  4:1629478/1‑151 (MQ=255)
                                                                                                                                     tACTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTg         >  4:1167736/1‑151 (MQ=255)
                                                                                                                                      aCTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCGCAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTg         <  4:567501/150‑1 (MQ=255)
                                                                                                                                      aCTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCt          <  2:1383857/149‑1 (MQ=255)
                                                                                                                                      aCTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTg         <  1:252792/150‑1 (MQ=255)
                                                                                                                                      aCTTCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGt        <  2:1130653/151‑1 (MQ=255)
                                                                                                                                         tCACAGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTaa      <  2:1481188/150‑1 (MQ=255)
                                                                                                                                            caGGTACGACAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCa  <  3:449092/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GTGCATCTTCACACTGACGGCAAACTGTCGCCGTGGAAATGTTCACACCTTTAATGACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCA  >  NC_000913/2837913‑2838203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: