Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,896,700 G→A P391S (CCG→TCG)  ygcS ← putative MFS sugar transporter; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,896,7000GA100.0% 429.9 / NA 107P391S (CCG→TCG) ygcSputative MFS sugar transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/55);  total (52/55)

Only 100 of 112 total aligned reads displayed.

CATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAAC  >  NC_000913/2896553‑2896847
                                                                                                                                                   |                                                                                                                                                   
cATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGacag                                                                                                                                                  <  4:1622720/151‑1 (MQ=255)
  ttCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGacagc                                                                                                                                                 <  4:920246/150‑1 (MQ=255)
  ttCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGacagc                                                                                                                                                 >  1:378567/1‑150 (MQ=255)
   tCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGacagc                                                                                                                                                 >  4:569327/1‑149 (MQ=255)
    tgCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAgg                                                                                                                                              >  2:229487/2‑151 (MQ=255)
    cGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGacagca                                                                                                                                                <  2:674498/149‑1 (MQ=255)
    cGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAgg                                                                                                                                              <  2:503252/151‑1 (MQ=255)
           cctACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGt                                                                                                                                         <  3:342098/149‑1 (MQ=255)
           cctACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTAc                                                                                                                                       >  4:1404968/1‑151 (MQ=255)
            ctACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACt                                                                                                                                      >  2:556609/1‑151 (MQ=255)
             tACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACtt                                                                                                                                     >  3:324405/1‑151 (MQ=255)
                  ttCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTAccgc                                                                                                                                >  3:263200/1‑151 (MQ=255)
                    cccGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTAccgcc                                                                                                                               <  2:1035475/150‑1 (MQ=255)
                     ccgccgCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACcgccgc                                                                                                                             >  4:974924/1‑151 (MQ=255)
                     ccgccgCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGACAGCAACCACGACAGCAGGCCAGTACTTACcgccgc                                                                                                                             >  2:863194/1‑151 (MQ=255)
                          gccACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCaa                                                                                                                        <  4:1203015/151‑1 (MQ=255)
                              ccaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCg                                                                                                                    <  2:114133/151‑1 (MQ=255)
                              ccaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCg                                                                                                                    >  4:1444770/1‑151 (MQ=255)
                              ccaGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCg                                                                                                                    <  4:688324/151‑1 (MQ=255)
                               caGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCg                                                                                                                    >  4:1490284/1‑150 (MQ=255)
                                aGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGa                                                                                                                   >  1:42738/1‑150 (MQ=255)
                                aGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGAc                                                                                                                  >  3:338554/1‑151 (MQ=255)
                                       gggCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGc                                                                                                           <  4:707426/151‑1 (MQ=255)
                                           tttAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTg                                                                                                       >  1:390852/1‑151 (MQ=255)
                                            ttAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTg                                                                                                       <  2:860672/150‑1 (MQ=255)
                                             tAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTgg                                                                                                      <  2:1204727/150‑1 (MQ=255)
                                             tAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGc                                                                                                     >  4:747648/1‑151 (MQ=255)
                                              aGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCa                                                                                                    <  4:1201649/151‑1 (MQ=255)
                                              aGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCa                                                                                                    <  2:238537/151‑1 (MQ=255)
                                                  tCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCaaaa                                                                                                 <  2:1114050/150‑1 (MQ=255)
                                                  tCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCaaaa                                                                                                 <  2:1214340/150‑1 (MQ=255)
                                                     ggTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAAcc                                                                                               <  4:115170/149‑1 (MQ=255)
                                                     ggTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAAc                                                                                                <  2:1170764/148‑1 (MQ=255)
                                                     gctGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCg                                                                                              >  2:432440/3‑150 (MQ=255)
                                                       tGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACg                                                                                           >  2:383961/1‑151 (MQ=255)
                                                          ccATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGccc                                                                                        >  3:248215/1‑151 (MQ=255)
                                                            aTAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGccc                                                                                        >  2:652226/1‑149 (MQ=255)
                                                            aTAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCag                                                                                      <  1:219465/151‑1 (MQ=255)
                                                            aTAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCa                                                                                       <  4:651736/150‑1 (MQ=255)
                                                               gCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcga                                                                                   <  4:373448/151‑1 (MQ=255)
                                                                ccAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcgag                                                                                  <  4:988126/151‑1 (MQ=255)
                                                                ccAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcg                                                                                    >  2:254889/1‑149 (MQ=255)
                                                                 cAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcgagc                                                                                 >  2:1447539/1‑151 (MQ=255)
                                                                 cAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcgagc                                                                                 <  3:60146/151‑1 (MQ=255)
                                                                  aGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCagcgagcg                                                                                >  3:265357/1‑151 (MQ=255)
                                                                    gTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGaa                                                                              <  4:543522/151‑1 (MQ=255)
                                                                      cacaACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACGGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAAt                                                                             <  4:871857/150‑1 (MQ=255)
                                                                           caaAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAg                                                                        <  4:34697/150‑1 (MQ=255)
                                                                           caaAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGt                                                                       >  4:326914/1‑151 (MQ=255)
                                                                            aaaaCCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTa                                                                      <  3:127050/151‑1 (MQ=255)
                                                                             aaaCCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAg                                                                     <  2:1270245/151‑1 (MQ=255)
                                                                              aaccaaccAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAgg                                                                    <  4:1492628/151‑1 (MQ=255)
                                                                               accaaccaACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGa                                                                   <  4:1162444/151‑1 (MQ=255)
                                                                                  aaccaacAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGaaaa                                                                <  2:295471/151‑1 (MQ=255)
                                                                                  aaccaacAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGaaa                                                                 >  4:1320799/1‑150 (MQ=255)
                                                                                    ccaacAACACTGTCGCCAGGAGCAATAAGGTGACTTCCATTCCCCCCTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAAc                                                               <  2:28223/150‑1 (MQ=255)
                                                                                     caacaaCACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACtt                                                             <  1:84050/151‑1 (MQ=255)
                                                                                     caacaaCACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACtt                                                             >  2:174827/1‑151 (MQ=255)
                                                                                        caacaCTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTcc                                                          >  4:184602/1‑151 (MQ=255)
                                                                                           cacTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGc                                                        >  2:571165/1‑150 (MQ=255)
                                                                                           cacTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACGGCGCCAGCCCCCGCGACAGCAGGCCAGTACTTGCCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAAGGTCAGTAGGAAAACTGTCCGc                                                        <  2:503402/150‑1 (MQ=255)
                                                                                            acTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGc                                                        >  4:174221/1‑149 (MQ=255)
                                                                                                   ccAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAAt                                               >  1:136899/1‑151 (MQ=255)
                                                                                                    cAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATg                                              >  2:1235072/1‑151 (MQ=255)
                                                                                                      ggAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGcc                                            >  2:1051049/1‑151 (MQ=255)
                                                                                                       gAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGccc                                           >  2:1465914/1‑151 (MQ=255)
                                                                                                        aGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGccc                                           >  3:330997/1‑150 (MQ=255)
                                                                                                         gCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCccac                                         <  2:697828/151‑1 (MQ=255)
                                                                                                         gCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCcca                                          >  1:303814/1‑150 (MQ=255)
                                                                                                          cAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCccacc                                        <  1:324388/151‑1 (MQ=255)
                                                                                                           aaTAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCccacc                                        >  4:907711/1‑150 (MQ=255)
                                                                                                            aTAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCccacc                                        >  4:935418/1‑149 (MQ=255)
                                                                                                             tAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCccacc                                        >  2:203718/1‑148 (MQ=255)
                                                                                                              aaGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCccacc                                        >  4:715224/1‑147 (MQ=255)
                                                                                                               aGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGAtt                                   >  4:1323294/1‑151 (MQ=255)
                                                                                                                 gTGACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTac                                 >  4:138916/1‑151 (MQ=255)
                                                                                                                   gACTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTact                                <  4:359721/150‑1 (MQ=255)
                                                                                                                     cTTGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTactga                              >  4:900793/1‑150 (MQ=255)
                                                                                                                      ttGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACttt                                                            <  1:425922/119‑1 (MQ=255)
                                                                                                                      ttGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTactga                              <  3:315179/149‑1 (MQ=255)
                                                                                                                       tGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTactgactg                           >  2:852484/1‑151 (MQ=255)
                                                                                                                       tGCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTactga                              <  2:899622/148‑1 (MQ=255)
                                                                                                                        gCATTCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGc                          <  4:1091862/151‑1 (MQ=255)
                                                                                                                           ttCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGcca                        <  2:568542/150‑2 (MQ=255)
                                                                                                                           ttCCCCACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCg                        >  1:101044/1‑150 (MQ=255)
                                                                                                                             ccccACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGaaa                     <  4:262524/151‑1 (MQ=255)
                                                                                                                             ccccACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGaa                      <  3:390869/150‑1 (MQ=255)
                                                                                                                               ccACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAAtg                   >  1:255299/1‑151 (MQ=255)
                                                                                                                               ccACTGCGCCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAAtg                   >  2:949051/1‑151 (MQ=255)
                                                                                                                                      gcCAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGc            <  4:147475/151‑1 (MQ=255)
                                                                                                                                       ccAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCt           >  2:91755/1‑151 (MQ=255)
                                                                                                                                        cAGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTg          <  4:1656487/151‑1 (MQ=255)
                                                                                                                                         aGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTg          >  2:85505/1‑150 (MQ=255)
                                                                                                                                         aGCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCAAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTg          >  4:1198065/1‑150 (MQ=255)
                                                                                                                                          gCACCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGa         >  4:957166/1‑150 (MQ=255)
                                                                                                                                            aCCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGa         >  2:1351226/1‑148 (MQ=255)
                                                                                                                                            aCCCACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAag       <  2:1102816/150‑1 (MQ=255)
                                                                                                                                               cACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAAtggtg                >  1:85773/1‑138 (MQ=255)
                                                                                                                                               cACGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAAtggtg                <  3:85777/138‑1 (MQ=255)
                                                                                                                                                aCGACAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAAc  >  4:603942/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATTCGCACCTCCTACATTTCCCGCCGCCACCAGCGGGAGGGCTTTAGTTTCTGGTGCCCATAGCCAGGTCACAACAAAACCAACCAACAACACTGTCGCCAGGAGCAATAAGGTGACTTGCATTCCCCACTGCGCCAGCACCCACGGCAGCAGGCCAGTACTTACCGCCGCGCCAAGTCGACTCATGGCAGTGGCAAAACCGACGCCCAGCGAGCGAATGTCAGTAGGAAAACTTTCCGCAGGCAAAATGCCCACCAGATTACTGACTGCCGAAATGGTGGTGCTGAAGAGAAC  >  NC_000913/2896553‑2896847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑