BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,028	T→C	T131A (ACC→GCC)	queE ←	7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,028	0	T	C	100.0%	405.6 / NA	103	T131A (ACC→GCC)	queE	7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (49/54); total (49/54)

Only 100 of 103 total aligned reads displayed.

TCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGTATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCT  >  NC_000913/2904884‑2905174

tCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCgg                                                                                                                                              >  2:480979/1‑151 (MQ=255)

 cAGCGTTGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCgg                                                                                                                                              <  3:222505/150‑1 (MQ=255)

   gCGTTGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGgtg                                                                                                                                            >  2:139903/1‑150 (MQ=255)

     gTTGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGc                                                                                                                                         <  4:1415776/151‑1 (MQ=255)

       tGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCa                                                                                                                                        <  4:1636954/150‑1 (MQ=255)

        gCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAg                                                                                                                                       <  2:446289/150‑1 (MQ=255)

        gCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGc                                                                                                                                      >  4:8722/1‑151 (MQ=255)

        gCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGc                                                                                                                                      >  3:77142/1‑151 (MQ=255)

          cAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGt                                                                                                                                    >  2:1677102/1‑151 (MQ=255)

          cAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGt                                                                                                                                    <  3:188845/151‑1 (MQ=255)

                ttCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTc                                                                                                                               <  2:1098639/150‑1 (MQ=255)

                 tCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCAt                                                                                                                             >  4:1674293/1‑151 (MQ=255)

                      cAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTa                                                                                                                        <  4:495740/151‑1 (MQ=255)

                       aGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTAc                                                                                                                       <  3:186911/151‑1 (MQ=255)

                           cTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACt                                                                                                                   >  2:363262/1‑151 (MQ=255)

                             tCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACt                                                                                                                   <  1:263667/149‑1 (MQ=255)

                              cAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGt                                                                                                                >  4:1037716/1‑151 (MQ=255)

                               aaTATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGtt                                                                                                               >  4:1587152/1‑151 (MQ=255)

                               aaTATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGtt                                                                                                               >  2:195791/1‑151 (MQ=255)

                                  atCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCg                                                                                                            >  2:490000/1‑151 (MQ=255)

                                    cgcgTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCTAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGa                                                                                                           <  4:624940/150‑1 (MQ=255)

                                    cgcgTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGa                                                                                                           <  2:917262/150‑1 (MQ=255)

                                    cgcgTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGAt                                                                                                          <  2:1625670/151‑1 (MQ=255)

                                      cgTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCt                                                                                                        <  4:1566657/151‑1 (MQ=255)

                                        tACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTgg                                                                                                      >  1:100546/1‑151 (MQ=255)

                                         aCGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGc                                                                                                     <  3:310637/151‑1 (MQ=255)

                                         aCGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGc                                                                                                     <  4:1588798/151‑1 (MQ=255)

                                            cgcCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGc                                                                                                  <  4:462197/151‑1 (MQ=255)

                                              ccccACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCt                                                                                                 >  2:682751/1‑150 (MQ=255)

                                              ccccACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCt                                                                                                 >  2:204198/1‑150 (MQ=255)

                                               cccACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTa                                                                                                >  4:1097188/1‑150 (MQ=255)

                                                ccACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTaaa                                                                                              <  3:475286/151‑1 (MQ=255)

                                                ccACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTaa                                                                                               >  2:263823/1‑150 (MQ=255)

                                                  aCTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAAc                                                                                            >  1:187209/1‑151 (MQ=255)

                                                         gCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCt                                                                                      <  4:620857/150‑1 (MQ=255)

                                                          cTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCt                                                                                      >  3:372982/1‑149 (MQ=255)

                                                            tGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTc                                                                                  >  4:505497/1‑151 (MQ=255)

                                                             gATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCg                                                                                 >  2:1143848/1‑151 (MQ=255)

                                                             gATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCg                                                                                 >  2:1348623/1‑151 (MQ=255)

                                                             gATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCg                                                                                 <  4:1661681/151‑1 (MQ=255)

                                                                 tCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGc                                                                              <  4:1355943/150‑1 (MQ=255)

                                                                 tCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGc                                                                              >  2:28286/1‑150 (MQ=255)

                                                                  cGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATTCGGTAACCCACGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCa                                                                             <  4:234879/150‑1 (MQ=255)

                                                                  cGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAg                                                                            <  4:294566/151‑1 (MQ=255)

                                                                      ggCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGAtca                                                                        <  1:362092/151‑1 (MQ=255)

                                                                          cgctcCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGAtcagtc                                                                     <  2:76350/150‑1 (MQ=255)

                                                                           gctcCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGAtcagtca                                                                    <  4:77616/150‑1 (MQ=255)

                                                                           gctcCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGAtcagtc                                                                     >  4:1046838/1‑149 (MQ=255)

                                                                             tcCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTg                                                                 >  4:30866/1‑151 (MQ=255)

                                                                              ccAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATcagtcagt                                                                  <  4:449914/149‑1 (MQ=255)

                                                                                aGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGgca                                                              <  2:615337/151‑1 (MQ=255)

                                                                                aGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGgc                                                               >  2:1062988/1‑150 (MQ=255)

                                                                                    ccTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCaa                                                          <  4:716150/151‑1 (MQ=255)

                                                                                       gtgACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAAt                                                        <  2:41484/150‑1 (MQ=255)

                                                                                          acaacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATg                                                    <  2:1525201/151‑1 (MQ=255)

                                                                                           caacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATg                                                    >  4:1617763/1‑150 (MQ=255)

                                                                                            aacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGaa                                                  <  4:1219379/151‑1 (MQ=255)

                                                                                            aacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGa                                                   >  2:1082736/1‑150 (MQ=255)

                                                                                            aacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGa                                                   >  4:385216/1‑150 (MQ=255)

                                                                                            aacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGa                                                   <  2:1181359/150‑1 (MQ=255)

                                                                                            aacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGa                                                   >  3:414352/1‑150 (MQ=255)

                                                                                            aacaCTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGa                                                   >  2:844934/1‑150 (MQ=255)

                                                                                              cacTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATg                                                >  4:703199/1‑151 (MQ=255)

                                                                                              cacTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATg                                                >  4:678932/1‑151 (MQ=255)

                                                                                              cacTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGGGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATg                                                >  3:93133/1‑151 (MQ=255)

                                                                                                 ttCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCa                                              >  4:1190167/1‑150 (MQ=255)

                                                                                                  tCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCa                                              <  4:1634244/149‑1 (MQ=255)

                                                                                                   cATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAg                                            <  3:30836/150‑1 (MQ=255)

                                                                                                   cATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAg                                            <  2:1387004/150‑1 (MQ=255)

                                                                                                   cATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAg                                            <  4:1101755/150‑1 (MQ=255)

                                                                                                      aGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTc                                        <  1:474418/151‑1 (MQ=255)

                                                                                                        ccgccgCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTcac                                      >  2:611928/1‑151 (MQ=255)

                                                                                                         cgccgcGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTcacc                                     >  4:1337245/1‑151 (MQ=255)

                                                                                                          gccgcGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTcacca                                    <  1:127040/151‑1 (MQ=255)

                                                                                                          gccgcGCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTcacc                                     >  1:205788/1‑150 (MQ=255)

                                                                                                            cgcgCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCaccacc                                  >  2:1653203/1‑151 (MQ=255)

                                                                                                            cgcgCATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCaccacc                                  <  3:187223/151‑1 (MQ=255)

                                                                                                             gcgcATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCAccc                                 >  4:1442220/1‑151 (MQ=255)

                                                                                                             gcgcATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCAccc                                 <  4:268069/151‑1 (MQ=255)

                                                                                                              cgcATGTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCg                                >  2:1556825/1‑151 (MQ=255)

                                                                                                                   gTTCAGCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATc                           <  4:1559091/151‑1 (MQ=255)

                                                                                                                       tgCTTTGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATcacc                        >  1:212039/2‑150 (MQ=255)

                                                                                                                          tttGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATg                                                    >  3:172995/1‑119 (MQ=255)

                                                                                                                          tttGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATg                                                    <  1:172981/119‑1 (MQ=255)

                                                                                                                          tttGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACAt                    >  2:1441761/1‑151 (MQ=255)

                                                                                                                           ttGGCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACcaca                     <  4:140141/149‑1 (MQ=255)

                                                                                                                              gCGATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCcg                <  3:141776/151‑1 (MQ=255)

                                                                                                                                gATACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCcgcg              <  4:618902/151‑1 (MQ=255)

                                                                                                                                  tACGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCcgcgcg            >  2:957046/1‑151 (MQ=255)

                                                                                                                                   aCGGTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCgg           <  4:101813/151‑1 (MQ=255)

                                                                                                                                     ggTAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGgtg         >  4:1542115/1‑151 (MQ=255)

                                                                                                                                       tAACCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGgtgt        >  1:297773/1‑150 (MQ=255)

                                                                                                                                        aaCCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTAt      <  2:504795/151‑1 (MQ=255)

                                                                                                                                         aCCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATc     <  4:1679136/151‑1 (MQ=255)

                                                                                                                                         aCCCAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATc     >  3:160938/1‑151 (MQ=255)

                                                                                                                                          cccAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATcc    <  4:753900/151‑1 (MQ=255)

                                                                                                                                           ccAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATccc   >  2:1461923/1‑151 (MQ=255)

                                                                                                                                            cAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCt  <  2:125860/151‑1 (MQ=255)

                                                                                                                                            cAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCt  >  4:1337525/1‑151 (MQ=255)

                                                                                                                                            cAGGCATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCt  <  4:830136/151‑1 (MQ=255)

TCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAATATCGCGTACGCGCCCCACTGGATGCTTGATTTCGTTGGCTCGCTCCAGTGCCTGTGACAACACTTCATAGCCGCCGCGCATGTTCAGCTTTGGCGATACGGTAACCCAGGTATTCGGTGTGCAGCGTACCTCATGAGTACCACTGGTTTCGATCTGGCAGCTAAAACCGTTCTTTTCGAGCAGATCAGTCAGTGGCAGCAAATCATGAATGCAAGGCTCACCACCCGTAATCACCACATGCCGCGCGGTGTATCCCT  >  NC_000913/2904884‑2905174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`