BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,908,834	T→C	Q278R (CAG→CGG)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,908,834	0	T	C	99.2%	495.3 / NA	132	Q278R (CAG→CGG)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (60/70); total (61/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TTGATGTTGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAT  >  NC_000913/2908690‑2908981

ttGATGTTGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTccc                                                                                                                                               >  1:210256/1‑151 (MQ=255)

ttGATGTTGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTcc                                                                                                                                                <  4:1450757/150‑1 (MQ=255)

   aTGTTGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCAtt                                                                                                                                            <  2:920617/151‑1 (MQ=255)

     gTTGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTc                                                                                                                                           <  4:1187853/150‑1 (MQ=255)

       tGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAACATAACCCGTTCCCATTCg                                                                                                                                          >  1:109097/1‑149 (MQ=255)

         aCGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGAc                                                                                                                                       >  4:1451791/1‑150 (MQ=255)

              gACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGAtt                                                                                                                                  <  2:1492089/150‑1 (MQ=255)

              gACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGAt                                                                                                                                   <  2:91844/149‑1 (MQ=255)

               acacGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCg                                                                                                                                >  4:380676/1‑151 (MQ=255)

                  cGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCtt                                                                                                                             <  4:1601015/151‑1 (MQ=255)

                   gATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTc                                                                                                                            <  2:324978/151‑1 (MQ=255)

                   gATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGAGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTc                                                                                                                            >  1:124563/1‑151 (MQ=255)

                    aTTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTcc                                                                                                                           <  4:653732/151‑1 (MQ=255)

                       cttcAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCggg                                                                                                                        >  2:263466/1‑151 (MQ=255)

                        ttcAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGc                                                                                                                       >  3:260504/1‑151 (MQ=255)

                         tcAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGc                                                                                                                       >  2:1251474/1‑150 (MQ=255)

                           aGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGt                                                                                                                    <  3:10703/151‑1 (MQ=255)

                              ccACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTa                                                                                                                 <  4:1413550/151‑1 (MQ=255)

                              ccACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTa                                                                                                                 <  1:13711/151‑1 (MQ=255)

                               cACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTa                                                                                                                 >  2:284713/1‑150 (MQ=255)

                                aCCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAg                                                                                                               <  3:210271/151‑1 (MQ=255)

                                  cGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCt                                                                                                             >  4:1065714/1‑151 (MQ=255)

                                   gtgtTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTg                                                                                                            >  2:1304790/1‑151 (MQ=255)

                                    tgtTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTg                                                                                                            >  4:642060/1‑150 (MQ=255)

                                         cAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGt                                                                                                      >  4:1015240/1‑151 (MQ=255)

                                           gTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGttt                                                                                                    <  4:398126/151‑1 (MQ=255)

                                             gCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTAc                                                                                                  >  4:831188/1‑151 (MQ=255)

                                              cTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTAc                                                                                                  <  1:18847/150‑1 (MQ=255)

                                                tCGATCACTGATTTATAAGCATCCGGCAGCTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACaa                                                                                                >  3:86567/1‑150 (MQ=255)

                                                 cGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAat                                                                                              >  2:493329/1‑151 (MQ=255)

                                                 cGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAat                                                                                              >  2:1676810/1‑151 (MQ=255)

                                                  gATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAat                                                                                              >  2:1448282/1‑150 (MQ=255)

                                                    tCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAatat                                                                                            >  4:912147/1‑150 (MQ=255)

                                                       cTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAAt                                                                                         <  2:47627/150‑1 (MQ=255)

                                                         gATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATc                                                                                        <  2:92996/149‑1 (MQ=255)

                                                          aTTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTc                                                                                     <  2:1149933/151‑1 (MQ=255)

                                                            ttATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAAtt                                                                                        <  4:1371745/146‑2 (MQ=255)

                                                            ttATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTcc                                                                                    <  4:152397/150‑1 (MQ=255)

                                                              ataAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAg                                                                                  <  2:87347/150‑1 (MQ=255)

                                                                aaGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGTGTTCTCGTCTTCGTAGATATTCTGTTCCCACGCGGACACACGCGCTTCCGCGCAGTTTCAGCCGCATCTTCCACAAACACCTTCGTCCAGccc                                                                               <  2:1131285/151‑1 (MQ=255)

                                                                   cATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTg                                                                            <  2:1407329/151‑1 (MQ=255)

                                                                    aTCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTg                                                                            >  2:1448563/1‑150 (MQ=255)

                                                                       cGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTg                                                                            >  4:1046871/1‑147 (MQ=255)

                                                                       cGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTg                                                                            >  2:1206454/1‑147 (MQ=255)

                                                                               cAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAAc                                                                  >  2:145200/1‑149 (MQ=255)

                                                                               cAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACa                                                                 <  1:325929/150‑1 (MQ=255)

                                                                               cAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAg                                                                <  2:969340/151‑1 (MQ=255)

                                                                                aaTGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAgg                                                               >  2:932087/1‑151 (MQ=255)

                                                                                 aTGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGc                                                              >  2:1336811/1‑151 (MQ=255)

                                                                                   gTACTTGCCGACCATACCGGTGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGcc                                                             <  2:1405740/150‑1 (MQ=255)

                                                                                   gTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGccc                                                            >  1:468315/1‑151 (MQ=255)

                                                                                         gCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAAt                                                       <  4:475712/150‑1 (MQ=255)

                                                                                           cGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAAtttt                                                    >  4:952757/1‑151 (MQ=255)

                                                                                            gACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAAtttt                                                    >  3:17066/1‑150 (MQ=255)

                                                                                               cATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCTATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAg                                                <  4:1621286/151‑1 (MQ=255)

                                                                                               cATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAg                                                <  1:69135/151‑1 (MQ=255)

                                                                                                aTACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTtat                                                  >  4:1522674/1‑148 (MQ=255)

                                                                                                  aCCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGAt                                              <  3:384655/150‑1 (MQ=255)

                                                                                                  aCCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGAt                                              <  2:1244198/150‑1 (MQ=255)

                                                                                                   ccGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATg                                             <  2:1486855/150‑1 (MQ=255)

                                                                                                    cGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATgg                                            >  1:465179/1‑150 (MQ=255)

                                                                                                    cGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATgg                                            >  2:1507425/1‑150 (MQ=255)

                                                                                                     gATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGaa                                          <  4:1518865/151‑1 (MQ=255)

                                                                                                        ggTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCg                                       >  4:1122971/1‑151 (MQ=255)

                                                                                                           gACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACg                                    >  2:504781/1‑151 (MQ=255)

                                                                                                           gACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACg                                    <  2:1426020/151‑1 (MQ=255)

                                                                                                            aCTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGt                                   >  2:1571478/1‑151 (MQ=255)

                                                                                                               tCACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCt                                 <  3:129870/150‑1 (MQ=255)

                                                                                                                cACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCttt                               <  1:122602/151‑1 (MQ=255)

                                                                                                                cACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCtt                                <  2:1290996/150‑1 (MQ=255)

                                                                                                                cACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCtt                                <  2:1077410/150‑1 (MQ=255)

                                                                                                                cACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCtt                                <  2:1396210/150‑1 (MQ=255)

                                                                                                                cACTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCtt                                <  3:390152/150‑1 (MQ=255)

                                                                                                                 aCTTACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTc                              >  4:619401/1‑151 (MQ=255)

                                                                                                                    tACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTc                              >  4:1160326/1‑148 (MQ=255)

                                                                                                                    tACCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTc                              >  4:156979/1‑148 (MQ=255)

                                                                                                                     aCCGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCaga                           <  4:569177/150‑1 (MQ=255)

                                                                                                                       cGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAgaga                         >  1:427524/1‑150 (MQ=255)

                                                                                                                       cGGGTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAgaga                         >  3:61920/1‑150 (MQ=255)

                                                                                                                        gggTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGaaa                       <  2:380118/151‑1 (MQ=255)

                                                                                                                         ggTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAAt                      <  2:1142896/151‑1 (MQ=255)

                                                                                                                         ggTTCGCTTCTTCGAAGATAACCAGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAAt                      >  4:1626103/1‑151 (MQ=255)

                                                                                                                          gTTCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATa                     <  4:462015/151‑1 (MQ=255)

                                                                                                                            tCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATaa                    <  3:414861/150‑1 (MQ=255)

                                                                                                                            tCGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATaa                    <  1:193498/150‑1 (MQ=255)

                                                                                                                             cGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAcc                  <  4:1573424/151‑1 (MQ=255)

                                                                                                                             cGCTTCTTCGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAcc                  >  4:1224093/1‑151 (MQ=255)

                                                                                                                                   ttcGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtttt            >  2:705561/1‑151 (MQ=255)

                                                                                                                                    tcGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt              <  4:252259/148‑1 (MQ=255)

                                                                                                                                    tcGAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGGCTTTCAGAGAAATAACCGCttgt            <  1:3102/150‑3 (MQ=255)

                                                                                                                                      gAAGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTcc         <  2:1064356/151‑1 (MQ=255)

                                                                                                                                        aGATAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCgg       <  4:1253050/151‑1 (MQ=255)

                                                                                                                                          aTAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGaa     <  4:1678846/151‑1 (MQ=255)

                                                                                                                                          aTAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGa      <  4:1049409/150‑1 (MQ=255)

                                                                                                                                           tAACCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAAc    >  2:413171/1‑151 (MQ=255)

                                                                                                                                            aaCCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAAc    >  2:56925/1‑150 (MQ=255)

                                                                                                                                            aaCCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAAc    >  2:301973/1‑150 (MQ=255)

                                                                                                                                            aaCCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACa   <  2:240027/151‑1 (MQ=255)

                                                                                                                                             aCCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt  <  1:19074/151‑1 (MQ=255)

                                                                                                                                             aCCCGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt  >  4:1247997/1‑151 (MQ=255)

TTGATGTTGACGCTGACACGATTCTTCAGCCCACCGTGTTTCAGTGCTTCGATCACTGATTTATAAGCATCCGGCAGTTCAATGTACTTGCCGACCATACCGATGGTGACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAT  >  NC_000913/2908690‑2908981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`