Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,912,357 T→C H432R (CAC→CGC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,912,3570TC100.0% 402.6 / NA 105H432R (CAC→CGC) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/50);  total (55/50)

Only 100 of 105 total aligned reads displayed.

ACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGC  >  NC_000913/2912209‑2912503
                                                                                                                                                    |                                                                                                                                                  
aCCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCgg                                                                                                                                                  >  4:763282/1‑151 (MQ=255)
 ccGAGGTTGGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGa                                                                                                                                                 <  2:1424235/151‑1 (MQ=255)
   gAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGAtg                                                                                                                                               >  4:784563/1‑151 (MQ=255)
    aGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGAtg                                                                                                                                               >  1:183895/1‑150 (MQ=255)
     gttttGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGAtgtg                                                                                                                                             <  2:632681/149‑1 (MQ=255)
      gTTTGGTTTTAACCAGTCACGGCTGGGGTTGGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTGGGTGAACGGCACAACGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTgg                                                                                                                                            <  2:505363/151‑1 (MQ=255)
        ttGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGACCGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGt                                                                                                                                           <  4:570633/150‑1 (MQ=255)
          gggTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTaa                                                                                                                                         <  3:26825/150‑1 (MQ=255)
          gggTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAg                                                                                                                                        <  2:886357/151‑1 (MQ=255)
            gTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCg                                                                                                                                      >  2:1009217/1‑151 (MQ=255)
                aaCCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGt                                                                                                                                  <  1:107211/151‑1 (MQ=255)
                aaCCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGt                                                                                                                                  >  1:201516/1‑151 (MQ=255)
                    aGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAg                                                                                                                              <  4:298340/151‑1 (MQ=255)
                     gTCACGGCTGGGGTTCGGTTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAgc                                                                                                                             <  4:486590/151‑1 (MQ=255)
                          ggCTGGGGTTCGGCTGTTTCTGGGGGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGGGTCCGACATCACTGCGGATGAGGTAAGCGAAGGCCAgcggcg                                                                                                                         >  2:649109/1‑150 (MQ=255)
                           gCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAgcggcg                                                                                                                         <  2:1673051/149‑1 (MQ=255)
                            cTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTg                                                                                                                      >  4:703779/1‑151 (MQ=255)
                              ggggTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGCTGTGGTAAGCGAAGTCCAGCGGCGTTGAt                                                                                                                    >  3:209852/1‑151 (MQ=255)
                              ggggTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGAt                                                                                                                    >  4:572946/1‑151 (MQ=255)
                                ggTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATcc                                                                                                                  >  4:343599/1‑151 (MQ=255)
                                 gTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATccc                                                                                                                 <  3:108850/151‑1 (MQ=255)
                                   tCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCTCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCg                                                                                                                <  4:1298837/150‑1 (MQ=255)
                                     ggCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGc                                                                                                               >  1:355122/1‑149 (MQ=255)
                                     ggCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCa                                                                                                              <  4:449783/150‑1 (MQ=255)
                                        tGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGc                                                                                                           >  2:755893/1‑150 (MQ=255)
                                           ttCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAAt                                                                                                       >  2:1618446/1‑151 (MQ=255)
                                            tCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATc                                                                                                      >  1:429234/1‑151 (MQ=255)
                                              tGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCa                                                                                                     >  2:1666066/1‑150 (MQ=255)
                                               gggTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAAc                                                                                                   <  4:966382/151‑1 (MQ=255)
                                                ggTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACg                                                                                                  >  4:1026359/1‑151 (MQ=255)
                                                ggTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACg                                                                                                  >  2:1415979/1‑151 (MQ=255)
                                                  tGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGAc                                                                                                >  4:1142156/1‑151 (MQ=255)
                                                    aTAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACa                                                                                               <  4:575670/150‑1 (MQ=255)
                                                      aaTTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCa                                                                                            <  2:640242/151‑1 (MQ=255)
                                                         ttCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCt                                                                                         >  3:138395/1‑151 (MQ=255)
                                                          tCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCtt                                                                                        >  4:1644812/1‑151 (MQ=255)
                                                           cAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCttt                                                                                       >  2:85982/1‑151 (MQ=255)
                                                              tCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCgg                                                                                    >  1:475431/1‑151 (MQ=255)
                                                               cTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGc                                                                                   >  4:1016553/1‑151 (MQ=255)
                                                                 gttCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCg                                                                                  <  2:356163/148‑1 (MQ=255)
                                                                 ggTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGc                                                                                   <  4:1068603/149‑1 (MQ=255)
                                                                 ggTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGt                                                                                 <  4:1555517/151‑1 (MQ=255)
                                                                   tCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTa                                                                                >  2:1475765/1‑150 (MQ=255)
                                                                      cccATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGa                                                                            <  3:284341/151‑1 (MQ=255)
                                                                       ccATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGAc                                                                           <  3:122776/151‑1 (MQ=255)
                                                                       ccATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGAc                                                                           >  2:1153947/1‑151 (MQ=255)
                                                                       ccATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGAc                                                                           >  4:1022261/1‑151 (MQ=255)
                                                                        cATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCTGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACg                                                                          >  4:1353774/1‑151 (MQ=255)
                                                                         aTCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGt                                                                         >  4:1480028/1‑151 (MQ=255)
                                                                             gCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGt                                                                         <  2:509470/147‑1 (MQ=255)
                                                                              cAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTacac                                                                     <  2:548545/150‑1 (MQ=255)
                                                                              cAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACAcc                                                                    >  3:282074/1‑151 (MQ=255)
                                                                               aGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTaca                                                                      <  2:1672524/148‑1 (MQ=255)
                                                                               aGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACAccc                                                                   >  4:217497/1‑151 (MQ=255)
                                                                               aGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACAccc                                                                   >  2:1498675/1‑151 (MQ=255)
                                                                                 cTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCgg                                                                 >  2:1534368/1‑151 (MQ=255)
                                                                                  tGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgt                                                                >  4:663308/1‑151 (MQ=255)
                                                                                   ggTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCg                                                                  <  1:357268/148‑1 (MQ=255)
                                                                                   ggTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtc                                                               <  2:1330767/151‑1 (MQ=255)
                                                                                   ggTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgt                                                                <  1:64023/150‑1 (MQ=255)
                                                                                     tAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtcgt                                                             >  2:527529/1‑151 (MQ=255)
                                                                                      aGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGgtcgtc                                                            >  1:35562/1‑151 (MQ=255)
                                                                                        gTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCa                                                           >  1:158892/1‑150 (MQ=255)
                                                                                         tGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCaa                                                          >  2:1603229/1‑150 (MQ=255)
                                                                                          gAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAg                                                        <  2:666758/151‑1 (MQ=255)
                                                                                            aCGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGAc                                                      >  4:607588/1‑151 (MQ=255)
                                                                                             cGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGAcc                                                     <  3:158906/151‑1 (MQ=255)
                                                                                               gCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTg                                                   >  4:1174664/1‑151 (MQ=255)
                                                                                                cacaATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCTGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGa                                                  <  2:1352087/151‑1 (MQ=255)
                                                                                                 acaATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGAc                                                 >  1:99301/1‑151 (MQ=255)
                                                                                                  caATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGAc                                                 <  4:1163443/150‑1 (MQ=255)
                                                                                                  caATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACt                                                >  2:848868/1‑151 (MQ=255)
                                                                                                    aTGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTAc                                              >  2:1147268/1‑151 (MQ=255)
                                                                                                    aTGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTAc                                              >  4:1393553/1‑151 (MQ=255)
                                                                                                     tGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACg                                             >  2:1283358/1‑151 (MQ=255)
                                                                                                      gcgcCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGGTGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACg                                             <  3:112088/150‑1 (MQ=255)
                                                                                                      gcgcCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACg                                             <  4:1375472/150‑1 (MQ=255)
                                                                                                      gcgcCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGt                                            <  1:391483/151‑1 (MQ=255)
                                                                                                       cgcccgccAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTa                                           <  2:783529/151‑1 (MQ=255)
                                                                                                              cAATTTTTGCCTCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTtcgtc                                    <  3:188833/151‑1 (MQ=255)
                                                                                                                      gCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCAttt                            <  2:1020941/151‑1 (MQ=255)
                                                                                                                      gCCCCGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCAtt                             <  2:969382/150‑1 (MQ=255)
                                                                                                                       ccccGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTc                           <  4:932127/151‑1 (MQ=255)
                                                                                                                       ccccGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTc                           <  2:1642809/151‑1 (MQ=255)
                                                                                                                        cccGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCg                          <  4:1124394/151‑1 (MQ=255)
                                                                                                                        cccGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCg                          <  4:766592/151‑1 (MQ=255)
                                                                                                                         ccGATGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCgg                         <  2:1389173/151‑2 (MQ=255)
                                                                                                                           gATGCAGCGGGGGCCGACATCAGTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCg                       >  2:1360639/1‑151 (MQ=255)
                                                                                                                             tGCAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCgg                      >  3:387002/1‑150 (MQ=255)
                                                                                                                               cAGCGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAAt                   <  4:1353236/151‑1 (MQ=255)
                                                                                                                                  cGGTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAg                <  2:320219/151‑1 (MQ=255)
                                                                                                                                   ggTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGc               >  2:185561/1‑151 (MQ=255)
                                                                                                                                   ggTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGc               >  4:1020475/1‑151 (MQ=255)
                                                                                                                                   ggTGTCCGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGc               <  4:842888/151‑1 (MQ=255)
                                                                                                                                        ccGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCAtct          >  1:270153/1‑151 (MQ=255)
                                                                                                                                         tgACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCAtctc         >  2:1225106/2‑151 (MQ=255)
                                                                                                                                         cGACATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCAtctc         <  2:1108607/151‑1 (MQ=255)
                                                                                                                                            cATCACTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTc      <  2:343088/151‑1 (MQ=255)
                                                                                                                                                aCTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGc  >  2:293859/1‑151 (MQ=255)
                                                                                                                                                aCTGCGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGc  >  2:651906/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGACATCACTGTGGATGTGGTAAGCGAAGTCCAGCGGCGTTGATCCCGCAGGCAAATCAACGACATCACCTTTCGGCGTAAAGACGTACACCCGGTCGTCAAAGACCTGACTACGTACTTCGTCGAGCATTTCGCCGGAATCAGCCATCTCTTCCTGC  >  NC_000913/2912209‑2912503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑