Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,915,854 T→C R266R (CGT→CGC)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,915,8540TC100.0% 512.3 / NA 139R266R (CGT→CGC) barAhybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (70/69);  total (70/69)

Only 100 of 142 total aligned reads displayed.

GTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACC  >  NC_000913/2915706‑2916001
                                                                                                                                                    |                                                                                                                                                   
gTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGa                                                                                                                                                   <  4:1242935/151‑1 (MQ=255)
  cgcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGaa                                                                                                                                                  <  4:748122/150‑1 (MQ=255)
   gcgGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAAc                                                                                                                                                <  4:1140322/151‑1 (MQ=255)
     gggCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGc                                                                                                                                              <  4:1422986/151‑1 (MQ=255)
       gCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTg                                                                                                                                            >  2:1380680/1‑151 (MQ=255)
                  gCCGGGCGGAAGGCTTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGCATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGCTCTGCGCGAAACGCTGGCGCAGATg                                                                                                                                   >  2:262337/1‑149 (MQ=255)
                    cGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGaa                                                                                                                                >  2:469526/1‑150 (MQ=255)
                    cGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGaa                                                                                                                                >  4:1455543/1‑150 (MQ=255)
                     gggTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAAt                                                                                                                              >  2:515955/1‑151 (MQ=255)
                       gTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGACAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTc                                                                                                                            <  4:1171203/151‑1 (MQ=255)
                         ggAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCCGCACAATATCGACCAGTCGACTTTCGATCTGCGCGTAACGCTGGAGGAGATGGaaactatg                                                                                                                          <  2:1306418/151‑6 (MQ=255)
                         ggAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAACATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTc                                                                                                                            >  4:772819/1‑149 (MQ=255)
                           aaGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGaa                                                                                                                        <  4:233372/151‑1 (MQ=255)
                            aGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGaa                                                                                                                        <  1:55668/150‑1 (MQ=255)
                             ggcgttATGCTCAGCGAGCTGGGTATGCTAAAACACCGTATCAACTCGATGGCAATGTCGCTCACTGCTTATCACGAACAGCTGCAGCACATTCCCGCCTAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGCAAATTCAGAACg                                                                                                                      >  1:360262/5‑151 (MQ=255)
                             ggATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACg                                                                                                                      >  4:1596403/1‑151 (MQ=255)
                              gATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGt                                                                                                                     >  1:335203/1‑151 (MQ=255)
                               aTTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGtt                                                                                                                    <  2:1102195/151‑1 (MQ=255)
                                tttATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTg                                                                                                                   >  4:1663716/1‑151 (MQ=255)
                                    tGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGt                                                                                                                <  3:367122/150‑1 (MQ=255)
                                     gCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGtt                                                                                                               >  2:1166146/1‑150 (MQ=255)
                                     gCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGtt                                                                                                               >  2:408778/1‑150 (MQ=255)
                                     gCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTa                                                                                                              <  2:1113440/151‑1 (MQ=255)
                                     gCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTa                                                                                                              <  4:767354/151‑1 (MQ=255)
                                     gCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTa                                                                                                              <  4:1537459/151‑1 (MQ=255)
                                     gCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTa                                                                                                              <  1:156042/151‑1 (MQ=255)
                                       tCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGtt                                                                                                               >  4:1012910/1‑148 (MQ=255)
                                           cGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTg                                                                                                        <  4:951522/151‑1 (MQ=255)
                                           cGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTg                                                                                                        <  1:139427/151‑1 (MQ=255)
                                           cGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTg                                                                                                        <  2:1610859/151‑1 (MQ=255)
                                               cTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCg                                                                                                     >  1:77269/1‑150 (MQ=255)
                                               cTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCg                                                                                                     >  2:1647793/1‑150 (MQ=255)
                                               cTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCg                                                                                                     >  1:42448/1‑150 (MQ=255)
                                                 ggATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGaa                                                                                                   <  2:22700/150‑1 (MQ=255)
                                                 ggATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGaa                                                                                                   <  4:1613517/150‑1 (MQ=255)
                                                 ggATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGa                                                                                                    <  4:24514/149‑1 (MQ=255)
                                                 ggATATGCTGAAAAACGGTATCAACGCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGaaa                                                                                                  <  1:32232/151‑1 (MQ=255)
                                                   atatGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGaaaaa                                                                                                <  3:169586/151‑1 (MQ=255)
                                                     atGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAg                                                                                               >  4:1300610/1‑150 (MQ=255)
                                                     atGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAg                                                                                               <  2:517923/150‑1 (MQ=255)
                                                       gCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGACGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAgcg                                                                                             >  1:436183/1‑150 (MQ=255)
                                                          gAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAgcgcg                                                                                           <  2:389718/149‑1 (MQ=255)
                                                           aaaaaCGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGccc                                                                                        >  3:275736/1‑151 (MQ=255)
                                                            aaaaCGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGccc                                                                                        >  4:635007/1‑150 (MQ=255)
                                                              aaCGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAgg                                                                                     >  4:1286762/1‑151 (MQ=255)
                                                              aaCGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAgg                                                                                     <  4:1167619/151‑1 (MQ=255)
                                                               aCGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAgg                                                                                     >  2:1642420/1‑150 (MQ=255)
                                                                   tATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAgcg                                                                                >  3:403722/1‑151 (MQ=255)
                                                                    aTCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAgcg                                                                                <  3:8450/150‑1 (MQ=255)
                                                                      cAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAgcggc                                                                              <  4:329846/150‑1 (MQ=255)
                                                                      cAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGACCTGGCGAAAAAGCGCGCCCAGGAAgcggc                                                                              <  2:959276/150‑1 (MQ=255)
                                                                       aaCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCTGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGgcgc                                                                            >  3:330000/1‑151 (MQ=255)
                                                                        aCTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGcgcg                                                                           >  2:490737/1‑151 (MQ=255)
                                                                         cTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGt                                                                          >  3:393408/1‑151 (MQ=255)
                                                                            gATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTAtt                                                                       <  4:394330/151‑1 (MQ=255)
                                                                            gATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTAtt                                                                       >  4:1467516/1‑151 (MQ=255)
                                                                            gATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTAtt                                                                       <  2:1136952/151‑1 (MQ=255)
                                                                             aTGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTa                                                                      <  2:302244/151‑1 (MQ=255)
                                                                                gCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAAt                                                                   <  4:1195847/151‑1 (MQ=255)
                                                                                  aaTGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATcc                                                                 >  2:927277/1‑151 (MQ=255)
                                                                                  aaTGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATcc                                                                 >  1:395820/1‑151 (MQ=255)
                                                                                   aTGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCg                                                                >  4:1373122/1‑151 (MQ=255)
                                                                                     gTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCg                                                                >  1:283271/1‑149 (MQ=255)
                                                                                     gTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGa                                                               <  4:71576/150‑1 (MQ=255)
                                                                                          tggctgCTTATCACGACGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCt                                                         >  2:291961/1‑151 (MQ=255)
                                                                                           ggctgCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCt                                                         <  4:1354453/150‑1 (MQ=255)
                                                                                           ggctgCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTg                                                        <  2:1193908/151‑1 (MQ=255)
                                                                                            gctgctTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTgg                                                       <  4:1393718/151‑1 (MQ=255)
                                                                                              tgctTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGc                                                      >  1:446704/1‑150 (MQ=255)
                                                                                                  tATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAat                                                  >  2:609526/1‑150 (MQ=255)
                                                                                                   aTCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAata                                                 >  4:327008/1‑150 (MQ=255)
                                                                                                       cGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTc                                             >  1:119850/1‑150 (MQ=255)
                                                                                                        gAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTcac                                           <  2:480123/151‑1 (MQ=255)
                                                                                                            agaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACg                                        >  2:174784/1‑150 (MQ=255)
                                                                                                            agaTGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACg                                        >  3:103954/1‑150 (MQ=255)
                                                                                                                gcagcaCAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCt                                    <  4:413079/150‑1 (MQ=255)
                                                                                                                gcagcaCAATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCt                                    <  3:42451/150‑1 (MQ=255)
                                                                                                                    cacaATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGt                                >  4:121204/1‑150 (MQ=255)
                                                                                                                    cacaATATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGt                                >  4:1557591/1‑150 (MQ=255)
                                                                                                                       aaTATCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTacac                            >  4:1519898/1‑151 (MQ=255)
                                                                                                                         tatCGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTAcacc                           >  2:1000940/1‑150 (MQ=255)
                                                                                                                           tctaCCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACt                        >  4:176625/4‑151 (MQ=255)
                                                                                                                            cGACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACt                        <  2:196949/150‑1 (MQ=255)
                                                                                                                             gACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTg                       >  3:278238/1‑150 (MQ=255)
                                                                                                                             gACCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTg                       >  4:1088384/1‑150 (MQ=255)
                                                                                                                              aCCAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGa                      >  2:1323110/1‑150 (MQ=255)
                                                                                                                                cAGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATg                   >  3:166207/1‑151 (MQ=255)
                                                                                                                                 aGGCGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATgg                  >  4:568662/1‑151 (MQ=255)
                                                                                                                                    cGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGgtgt               >  1:288435/1‑151 (MQ=255)
                                                                                                                                    cGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGgtgt               >  4:102693/1‑151 (MQ=255)
                                                                                                                                    cGACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGgtgt               <  2:1614322/151‑1 (MQ=255)
                                                                                                                                     gACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGtt              >  4:617235/1‑151 (MQ=255)
                                                                                                                                     gACGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGtt              >  2:635327/1‑151 (MQ=255)
                                                                                                                                       cGTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTAt            >  4:707081/1‑151 (MQ=255)
                                                                                                                                        gTCCGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTAtt           <  2:1176948/151‑1 (MQ=255)
                                                                                                                                          ccGATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTgg         >  2:894027/1‑151 (MQ=255)
                                                                                                                                            gATCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCt       >  3:145467/1‑151 (MQ=255)
                                                                                                                                             aTCTGCGCGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCtt      >  4:1675794/1‑151 (MQ=255)
                                                                                                                                                 gcgcGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTa    <  3:283287/149‑1 (MQ=255)
                                                                                                                                                 gcgcGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTAcc  <  4:901351/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTCGCGGGCAACTCGACAGCCGGGTGGAAGGATTTATGCTCGGCGAGCTGGATATGCTGAAAAACGGTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACC  >  NC_000913/2915706‑2916001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑