Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,044,702 T→C S347S (TCT→TCC)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,044,7020TC100.0% 461.7 / NA 122S347S (TCT→TCC) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/68);  total (54/68)

Only 100 of 122 total aligned reads displayed.

GGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTAC  >  NC_000913/3044556‑3044849
                                                                                                                                                  |                                                                                                                                                   
ggCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACt                                                                                                                                                 >  4:894005/1‑151 (MQ=255)
ggCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACt                                                                                                                                                 >  2:488648/1‑151 (MQ=255)
   gATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTggg                                                                                                                                              >  4:1387326/1‑151 (MQ=255)
   gATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTggg                                                                                                                                              >  3:162964/1‑151 (MQ=255)
     tCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGc                                                                                                                                            >  3:381115/1‑151 (MQ=255)
      cTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCt                                                                                                                                           >  3:2545/1‑151 (MQ=255)
       tGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTg                                                                                                                                          >  3:106376/1‑151 (MQ=255)
        ggATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTggg                                                                                                                                              <  4:164756/146‑1 (MQ=255)
        ggATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTg                                                                                                                                          <  2:540308/150‑1 (MQ=255)
        ggATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTg                                                                                                                                          <  2:26481/150‑1 (MQ=255)
          aTGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCa                                                                                                                                       <  4:310578/151‑1 (MQ=255)
            gtgCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAg                                                                                                                                      <  4:498573/150‑1 (MQ=255)
              gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGAtt                                                                                                                                   <  4:152792/151‑1 (MQ=255)
                   gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATcc                                                                                                                              <  4:372788/151‑1 (MQ=255)
                     gAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCa                                                                                                                             <  2:699934/150‑1 (MQ=255)
                      aaGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAg                                                                                                                            >  3:73485/1‑150 (MQ=255)
                       aGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTa                                                                                                                          <  2:158445/151‑1 (MQ=255)
                         gCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAgg                                                                                                                        <  2:51934/151‑1 (MQ=255)
                            ccTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCt                                                                                                                     >  1:183235/1‑151 (MQ=255)
                            ccTGCGATTATCTTGGTTCCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCt                                                                                                                     <  2:1050675/151‑1 (MQ=255)
                              tGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTgc                                                                                                                   >  4:739073/1‑151 (MQ=255)
                               gCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTg                                                                                                                    <  4:578273/149‑1 (MQ=255)
                                 gATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCt                                                                                                                <  4:789156/151‑1 (MQ=255)
                                  aTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCt                                                                                                                >  4:6526/1‑150 (MQ=255)
                                  aTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTa                                                                                                               <  2:1320572/151‑1 (MQ=255)
                                    tATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTAt                                                                                                              <  2:307551/150‑1 (MQ=255)
                                    tATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTAt                                                                                                              <  4:477484/150‑1 (MQ=255)
                                     aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGATGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGc                                                                                                            >  3:93481/1‑151 (MQ=255)
                                     aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGACGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGCTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATg                                                                                                             >  4:559227/1‑150 (MQ=255)
                                     aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATg                                                                                                             <  1:317154/150‑1 (MQ=255)
                                     aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGc                                                                                                            >  4:1024865/1‑151 (MQ=255)
                                          ggTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACttt                                                                                                       <  4:285748/151‑1 (MQ=255)
                                          ggTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACttt                                                                                                       >  4:712037/1‑151 (MQ=255)
                                           gTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTg                                                                                                      >  1:452124/1‑151 (MQ=255)
                                             ttCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGc                                                                                                     <  3:396545/150‑1 (MQ=255)
                                                 gCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACt                                                                                                <  1:169474/151‑1 (MQ=255)
                                                  cTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTg                                                                                               >  2:393242/1‑151 (MQ=255)
                                                    aTTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTg                                                                                               <  4:1297506/149‑1 (MQ=255)
                                                    aTTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTg                                                                                               >  4:1648082/1‑149 (MQ=255)
                                                    aTTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGt                                                                                              >  1:151178/1‑150 (MQ=255)
                                                      tACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                           >  2:471457/1‑151 (MQ=255)
                                                      tACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATg                                                                                           >  4:1438339/1‑151 (MQ=255)
                                                        cATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAg                                                                                         <  4:1062495/151‑1 (MQ=255)
                                                             ccAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTa                                                                                    <  4:119382/151‑1 (MQ=255)
                                                             ccAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTa                                                                                    <  4:588804/151‑1 (MQ=255)
                                                              cAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTAt                                                                                   <  2:1499024/151‑1 (MQ=255)
                                                              cAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTAt                                                                                   >  1:32012/1‑151 (MQ=255)
                                                               aaTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATc                                                                                  <  2:7473/151‑1 (MQ=255)
                                                               aaTGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATc                                                                                  >  3:384127/1‑151 (MQ=255)
                                                                 tGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATc                                                                                  >  2:338687/1‑149 (MQ=255)
                                                                   cAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCagag                                                                              <  3:368219/151‑1 (MQ=255)
                                                                      tGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGcc                                                                           >  3:224633/1‑151 (MQ=255)
                                                                       gAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGcc                                                                           <  2:1139959/150‑1 (MQ=255)
                                                                       gAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCg                                                                          <  4:1475489/151‑1 (MQ=255)
                                                                         aGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCt                                                                        >  2:226662/1‑151 (MQ=255)
                                                                              gAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGttt                                                                    >  3:230936/1‑150 (MQ=255)
                                                                              gAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGttt                                                                    >  4:1553816/1‑150 (MQ=255)
                                                                              gAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGttt                                                                    >  2:152976/1‑150 (MQ=255)
                                                                              gAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGttt                                                                    >  1:363699/1‑150 (MQ=255)
                                                                               aaGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTAt                                                                  <  4:211677/151‑1 (MQ=255)
                                                                                  gcggcACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAATCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGt                                                               <  4:799218/151‑1 (MQ=255)
                                                                                      cACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaa                                                           <  1:182702/151‑1 (MQ=255)
                                                                                      cACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaa                                                           <  4:1383528/151‑1 (MQ=255)
                                                                                      cACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaa                                                           <  3:278838/151‑1 (MQ=255)
                                                                                       aCCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaaa                                                          <  2:285893/151‑1 (MQ=255)
                                                                                       aCCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaa                                                           >  2:302889/1‑150 (MQ=255)
                                                                                        ccGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGaaaa                                                         <  2:6513/151‑1 (MQ=255)
                                                                                            cgatgcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGt                                                     <  1:181332/151‑1 (MQ=255)
                                                                                            cgatgcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGt                                                     <  3:5972/151‑1 (MQ=255)
                                                                                            cgatgcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGt                                                     <  2:395356/151‑1 (MQ=255)
                                                                                             gatgcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtt                                                    <  4:898209/151‑1 (MQ=255)
                                                                                             gatgcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtt                                                    <  2:364190/151‑1 (MQ=255)
                                                                                             gatgcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACCTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtt                                                    <  1:430448/151‑1 (MQ=255)
                                                                                                gcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtttt                                                  <  1:302574/150‑1 (MQ=255)
                                                                                                gcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtttt                                                  <  4:311348/150‑1 (MQ=255)
                                                                                                gcgatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGtttt                                                  <  4:1036747/150‑1 (MQ=255)
                                                                                                  gatCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGgc                                               >  4:1106501/1‑151 (MQ=255)
                                                                                                       ctGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGACCTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCt                                            <  3:183249/149‑1 (MQ=255)
                                                                                                       ctGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGgc                                               <  4:878139/146‑1 (MQ=255)
                                                                                                         ggTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCtt                                           <  2:762374/148‑1 (MQ=255)
                                                                                                         ggTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCtt                                           <  4:33478/148‑1 (MQ=255)
                                                                                                               gAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACaa                                    >  4:355959/1‑149 (MQ=255)
                                                                                                                aaGGCAGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGt                                 >  1:247266/1‑151 (MQ=255)
                                                                                                                     aGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGga                              >  3:439700/1‑149 (MQ=255)
                                                                                                                     aGCGTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGga                              >  2:869427/1‑149 (MQ=255)
                                                                                                                        gTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGgaaga                           >  1:474913/1‑149 (MQ=255)
                                                                                                                        gTACCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGaagaag                         <  2:441243/151‑1 (MQ=255)
                                                                                                                          aCCAAACCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAagaaga                        <  4:422463/150‑1 (MQ=255)
                                                                                                                             aaaCCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgc                    >  1:404733/1‑151 (MQ=255)
                                                                                                                             aaaCCCGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgc                    <  2:1341845/151‑1 (MQ=255)
                                                                                                                                cccGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgcagc                 <  4:889948/151‑1 (MQ=255)
                                                                                                                                 ccGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGgcagc                 >  2:1207027/1‑150 (MQ=255)
                                                                                                                                  cGTATGTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCAt               >  2:1617917/1‑151 (MQ=255)
                                                                                                                                       gTTAAAGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAacg          <  3:72576/151‑1 (MQ=255)
                                                                                                                                          aaaGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAacgacg       >  4:1064924/1‑151 (MQ=255)
                                                                                                                                          aaaGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAacgac        <  4:390880/150‑1 (MQ=255)
                                                                                                                                          aaaGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAacgac        <  4:171316/150‑1 (MQ=255)
                                                                                                                                           aaGCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAAcgacga      <  4:153240/151‑1 (MQ=255)
                                                                                                                                             gCATCCGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACt    >  3:124774/1‑151 (MQ=255)
                                                                                                                                               aTCCGACTGGGGCTGGCAGATTGATACAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTAc  >  4:586805/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATCCAGTAGGTCTGCGCTATGCACTTTGCGAACTGTATGAGCGTTATCAGAGGCCGCTGTTTATTGTCGAAAACGGTTTTGGCGCTTACGACAAAGTGGAAGAAGATGGCAGCATCAACGACGACTAC  >  NC_000913/3044556‑3044849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑