Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,085,843 C→T Q23Q (CAG→CAA speA ← biosynthetic arginine decarboxylase, PLP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,085,8430CT99.2% 521.8 / NA 133Q23Q (CAG→CAAspeAbiosynthetic arginine decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (65/67);  minor base G (1/0);  total (66/67)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCCTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAACCCATAA  >  NC_000913/3085696‑3085989
                                                                                                                                                   |                                                                                                                                                  
   aTCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATg                                                                                                                                              <  2:634532/151‑1 (MQ=255)
   aTCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATg                                                                                                                                              <  2:1041062/151‑1 (MQ=255)
      gACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAg                                                                                                                                           <  4:1549136/151‑1 (MQ=255)
          cgAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCgta                                                                                                                                       <  2:381784/151‑1 (MQ=255)
          cgAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCgta                                                                                                                                       <  4:23966/151‑1 (MQ=255)
              cTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTac                                                                                                                                   >  2:270381/1‑151 (MQ=255)
               ttCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCgtagta                                                                                                                                    >  3:100884/1‑149 (MQ=255)
               ttCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCgtagta                                                                                                                                    <  1:100870/149‑1 (MQ=255)
               ttCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTac                                                                                                                                   >  2:340268/1‑150 (MQ=255)
                     gACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCgtgt                                                                                                                            <  4:300748/151‑1 (MQ=255)
                       cGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTc                                                                                                                          >  4:1493306/1‑151 (MQ=255)
                           cgggtccgggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGccc                                                                                                                      <  2:143274/151‑1 (MQ=255)
                           cgggtccgggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGccc                                                                                                                      <  2:1612860/151‑1 (MQ=255)
                             ggtccgggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGc                                                                                                                    <  4:825403/151‑1 (MQ=255)
                             ggtccgggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGc                                                                                                                    <  2:1035875/151‑1 (MQ=255)
                                ccgggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTg                                                                                                                  >  3:28603/1‑150 (MQ=255)
                                  gggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACg                                                                                                               >  4:1237022/1‑151 (MQ=255)
                                   ggCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTGCGCCCGCTGGCGa                                                                                                              <  1:436459/151‑1 (MQ=255)
                                        acGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGc                                                                                                          >  4:815739/1‑150 (MQ=255)
                                        acGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGc                                                                                                          >  4:415009/1‑150 (MQ=255)
                                         cGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCa                                                                                                         <  2:1344257/150‑1 (MQ=255)
                                          gCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCaa                                                                                                        >  4:1630900/1‑150 (MQ=255)
                                           cTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTCCACCGTGTTCGCCCGCTGACGAAGGCAAAc                                                                                                      >  2:1570179/1‑151 (MQ=255)
                                           cTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAAc                                                                                                      >  2:791530/1‑151 (MQ=255)
                                             aaTGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAAccc                                                                                                    >  2:496087/1‑151 (MQ=255)
                                             aaTGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAAc                                                                                                      <  2:1242351/149‑1 (MQ=255)
                                                   gCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGAc                                                                                              <  2:1348339/151‑1 (MQ=255)
                                                     ccAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACAt                                                                                            <  2:676416/151‑1 (MQ=255)
                                                       aGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATgt                                                                                          >  4:759287/1‑151 (MQ=255)
                                                        gCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATgtc                                                                                         >  4:947894/1‑151 (MQ=255)
                                                         cTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATgtcg                                                                                        >  2:638918/1‑151 (MQ=255)
                                                                 cGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATg                                                                                           <  1:160776/140‑1 (MQ=255)
                                                                 cGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACAt                                                                                <  2:864001/151‑1 (MQ=255)
                                                                 cGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACAt                                                                                >  2:702103/1‑151 (MQ=255)
                                                                 cGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACAt                                                                                >  2:1218317/1‑151 (MQ=255)
                                                                     aTAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCg                                                                            >  2:200947/1‑151 (MQ=255)
                                                                     aTAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCg                                                                            <  2:1634652/151‑1 (MQ=255)
                                                                     aTAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCg                                                                            >  2:1470476/1‑151 (MQ=255)
                                                                       agtagtTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGa                                                                           >  4:1350539/1‑150 (MQ=255)
                                                                        gtagtTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAAc                                                                         <  4:1385679/151‑1 (MQ=255)
                                                                         tagtTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAAcc                                                                        >  3:235215/1‑151 (MQ=255)
                                                                            ttATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTc                                                                      >  2:955393/1‑150 (MQ=255)
                                                                            ttATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTc                                                                      >  4:332251/1‑150 (MQ=255)
                                                                              aTTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCaaa                                                                   <  2:414414/151‑1 (MQ=255)
                                                                              aTTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCaa                                                                    <  3:205270/150‑1 (MQ=255)
                                                                                tGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAatt                                                                 >  1:379138/1‑151 (MQ=255)
                                                                                 gCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAatta                                                                <  1:360690/151‑1 (MQ=255)
                                                                                  ccccACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAattat                                                               <  4:66155/151‑1 (MQ=255)
                                                                                  ccccACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAattat                                                               <  1:405163/151‑1 (MQ=255)
                                                                                    ccaccaGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAAttattt                                                             >  4:1516953/1‑151 (MQ=255)
                                                                                    ccaccaGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAAttattt                                                             >  2:393497/1‑151 (MQ=255)
                                                                                    ccaccaGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAAttattt                                                             <  4:1574787/151‑1 (MQ=255)
                                                                                      accaGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAAttattt                                                             >  2:624389/1‑149 (MQ=255)
                                                                                       ccaGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAActt                                                                 >  2:1083331/1‑144 (MQ=255)
                                                                                       ccaGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAAttattttat                                                          >  2:1117678/1‑151 (MQ=255)
                                                                                        caGGCAATAATGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAAttattttatt                                                         >  2:848298/1‑151 (MQ=255)
                                                                                           gCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTttatta                                                        >  1:303564/1‑149 (MQ=255)
                                                                                             aaTATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAgtg                                                    >  4:816889/1‑151 (MQ=255)
                                                                                             aaTATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAgtg                                                    <  4:1484241/151‑1 (MQ=255)
                                                                                             aaTATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAgt                                                     >  1:204670/1‑150 (MQ=255)
                                                                                              atatTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAgtgt                                                   <  1:366581/151‑1 (MQ=255)
                                                                                               tatTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAatt                                                                 >  3:39477/1‑136 (MQ=255)
                                                                                                atTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTa                                                  <  3:424024/150‑1 (MQ=255)
                                                                                                   gTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAAc                                              <  4:409234/151‑1 (MQ=255)
                                                                                                      aGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGt                                           <  2:1147795/151‑1 (MQ=255)
                                                                                                           gcagcaTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTaaag                                      <  4:460648/151‑3 (MQ=255)
                                                                                                           gcagcaTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACg                                      <  3:472210/151‑1 (MQ=255)
                                                                                                           gcagcaTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACg                                      <  4:338480/151‑1 (MQ=255)
                                                                                                           gcagcaTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACg                                      <  2:1524248/151‑1 (MQ=255)
                                                                                                              gcaTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACt                                   >  4:599618/1‑151 (MQ=255)
                                                                                                                aTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTAt                                 >  2:1678573/1‑151 (MQ=255)
                                                                                                                aTCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTAt                                 >  1:470019/1‑151 (MQ=255)
                                                                                                                    tGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTAt                                 <  3:409313/147‑1 (MQ=255)
                                                                                                                    tGCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGc                              >  4:1244839/1‑150 (MQ=255)
                                                                                                                     gCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGc                              >  2:429227/1‑149 (MQ=255)
                                                                                                                     gCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAg                            <  2:188162/151‑1 (MQ=255)
                                                                                                                     gCTGGCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAg                            <  2:1543281/151‑1 (MQ=255)
                                                                                                                       ttgCTTCCTGGGAGCTCATTGCAACCTCTTGCATGGCGCGTAGTCCACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGcc                          >  4:977457/3‑151 (MQ=255)
                                                                                                                        ggCTTCCTGGGAGCTCATTGCAACCGCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCt                         <  2:806288/151‑1 (MQ=255)
                                                                                                                           ttCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTgg                       >  4:248363/1‑150 (MQ=255)
                                                                                                                           ttCCTGGGAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTgg                       >  2:1123732/1‑150 (MQ=255)
                                                                                                                                gggAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGc                 <  2:1491437/151‑1 (MQ=255)
                                                                                                                                 ggAGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGc                 <  4:261984/150‑1 (MQ=255)
                                                                                                                                   aGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGAt              <  2:700635/151‑1 (MQ=255)
                                                                                                                                   aGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGAt              <  4:785689/151‑1 (MQ=255)
                                                                                                                                   aGCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGAt              <  4:496784/151‑1 (MQ=255)
                                                                                                                                    gCTCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGAt              >  3:35129/1‑150 (MQ=255)
                                                                                                                                    gCTCATTGCAACCTCTTGCATGGAGCGTAGCACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGAt              >  4:1335503/1‑150 (MQ=255)
                                                                                                                                      tCATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATaac           >  1:289572/1‑151 (MQ=255)
                                                                                                                                       cATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATaac           <  3:251910/150‑1 (MQ=255)
                                                                                                                                       cATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATaac           <  2:426910/150‑1 (MQ=255)
                                                                                                                                       cATTGCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATaa            <  4:495855/149‑1 (MQ=255)
                                                                                                                                          tGCAACCTCGTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAAcc       >  2:1172000/1‑151 (MQ=255)
                                                                                                                                           gCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAAccc      <  2:957749/151‑1 (MQ=255)
                                                                                                                                           gCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAAccc      >  2:559671/1‑151 (MQ=255)
                                                                                                                                           gCAACCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTAGTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAAccc      <  2:542483/151‑1 (MQ=255)
                                                                                                                                             aaCCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAACCCa     >  1:29728/1‑150 (MQ=255)
                                                                                                                                             aaCCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAACCCAt    >  2:817735/1‑151 (MQ=255)
                                                                                                                                              aCCTCTTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAACCCAt    >  4:1212738/1‑150 (MQ=255)
                                                                                                                                               cctcttGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAACCCATaa  <  1:424010/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAGTTATTGCCCCACCAGGCAATATTGTAAGTACGCAGCATCTTGCTGGCTTCCTGGGAGCTCATTGCAACCTCCTGCATGGAGCGTAGTACACCGTGTTCGCCCGCTGACGAAGGCAAACCCATAGACATGTCGTCAGACATAGCGAACCTCAAATTATTTTATTAAGTGTAAAACAGTTAACGACTATCGCAGCCTGGAACTGCGATAACAACCCATAA  >  NC_000913/3085696‑3085989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: