Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,094,280 C→T V267V (GTG→GTA yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,094,2800CT99.0% 393.1 / ‑6.8 102V267V (GTG→GTAyggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/53);  minor base G (1/0);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGCCAGCACCAA  >  NC_000913/3094133‑3094423
                                                                                                                                                   |                                                                                                                                               
aCTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACt                                                                                                                                              >  1:436968/1‑151 (MQ=255)
 ctctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACtt                                                                                                                                             >  4:678081/1‑151 (MQ=255)
   ctgctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACtt                                                                                                                                             <  1:269442/149‑1 (MQ=255)
     gctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAg                                                                                                                                         <  4:882253/151‑1 (MQ=255)
     gctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAg                                                                                                                                         <  2:112222/151‑1 (MQ=255)
       tgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCt                                                                                                                                       >  3:428566/1‑151 (MQ=255)
        gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCtt                                                                                                                                      <  2:71104/151‑1 (MQ=255)
        gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCtt                                                                                                                                      <  2:525636/151‑1 (MQ=255)
        gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCtt                                                                                                                                      <  1:385160/151‑1 (MQ=255)
        gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCt                                                                                                                                       <  4:1484032/150‑1 (MQ=255)
        gAAACGTTATCATCCCCACCCGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCtt                                                                                                                                      >  4:1510152/1‑151 (MQ=255)
         aaaCGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCttt                                                                                                                                     >  2:58746/1‑151 (MQ=255)
           aCGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTtg                                                                                                                                   >  1:234578/1‑151 (MQ=255)
            cGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTtgt                                                                                                                                  <  4:54832/151‑1 (MQ=255)
            cGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTtgt                                                                                                                                  >  4:656778/1‑151 (MQ=255)
               tATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTCCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTtgt                                                                                                                                  <  1:338313/148‑1 (MQ=255)
                   atcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCa                                                                                                                           <  4:1676184/151‑1 (MQ=255)
                           ctgctgCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCg                                                                                                                    >  2:1127090/1‑150 (MQ=255)
                             gctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTaa                                                                                                                 <  3:267727/151‑1 (MQ=255)
                             gctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTa                                                                                                                  >  4:465096/1‑150 (MQ=255)
                                gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACt                                                                                                              >  2:1078366/1‑151 (MQ=255)
                                 cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACt                                                                                                              >  2:1262297/1‑150 (MQ=255)
                                 cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACt                                                                                                              <  2:1092861/150‑1 (MQ=255)
                                  ccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTAc                                                                                                            >  4:1132365/1‑151 (MQ=255)
                                    ggTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCt                                                                                                          <  3:345157/151‑1 (MQ=255)
                                      tttGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGGGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTAcc                                                                                                           >  2:233931/1‑148 (MQ=255)
                                         gAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGcc                                                                                                       >  1:136116/1‑149 (MQ=255)
                                           aTAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGtt                                                                                                   <  4:1076888/151‑1 (MQ=255)
                                           aTAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGtt                                                                                                   >  3:337377/1‑151 (MQ=255)
                                           aTAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGtt                                                                                                   >  2:23790/1‑151 (MQ=255)
                                            tAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTg                                                                                                  >  2:908949/1‑151 (MQ=255)
                                             aaCATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTg                                                                                                  >  2:1437978/1‑150 (MQ=255)
                                             aaCATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGa                                                                                                 <  4:64452/151‑1 (MQ=255)
                                              aCATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAt                                                                                                <  2:1314867/151‑1 (MQ=255)
                                              aCATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAt                                                                                                <  2:1001988/151‑1 (MQ=255)
                                                 tGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCCCCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTa                                                                                              <  2:531633/150‑1 (MQ=255)
                                                  gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACg                                                                                            <  3:136130/151‑1 (MQ=255)
                                                     gTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTGCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCAc                                                                                         <  3:361605/151‑1 (MQ=255)
                                                       aaCTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACg                                                                                        <  2:412675/150‑1 (MQ=255)
                                                       aaCTGGAGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCATTTCAAATAGGGCCACGCGACCTTCCTGTTTATCTACTTCCAGCTTTTGAGACAGCACTGCCCGTAAACTATTGTCCATGTGTTTACGCACgg                                                                                       >  2:449381/1‑151 (MQ=255)
                                                          tggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGt                                                                                    >  3:107969/1‑151 (MQ=255)
                                                          tggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGt                                                                                    >  2:366911/1‑151 (MQ=255)
                                                           ggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgggg                                                                                     <  2:920784/149‑1 (MQ=255)
                                                           ggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGt                                                                                    <  3:404245/150‑1 (MQ=255)
                                                              gggTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCtt                                                                                 <  2:1436417/150‑1 (MQ=255)
                                                               ggTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCtttt                                                                               >  4:885226/1‑151 (MQ=255)
                                                               ggTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCtttt                                                                               >  4:588082/1‑151 (MQ=255)
                                                                  tttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTcc                                                                            >  2:1274141/1‑151 (MQ=255)
                                                                  tttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTcc                                                                            >  4:353783/1‑151 (MQ=255)
                                                                   ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCt                                                                           >  4:1218829/1‑151 (MQ=255)
                                                                   ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCt                                                                           >  1:289009/1‑151 (MQ=255)
                                                                    tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCt                                                                           <  4:1312280/150‑1 (MQ=255)
                                                                    tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTg                                                                          >  4:419683/1‑151 (MQ=255)
                                                                     cccTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTgc                                                                         <  2:784427/151‑1 (MQ=255)
                                                                     cccTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTg                                                                          <  4:167566/150‑1 (MQ=255)
                                                                      ccTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTgcg                                                                        <  1:248071/151‑1 (MQ=255)
                                                                        ttCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGcc                                                                      >  1:251341/1‑151 (MQ=255)
                                                                         tCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTgcgc                                                                       <  2:1587609/149‑1 (MQ=255)
                                                                            cgAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGa                                                                   >  2:1438977/1‑150 (MQ=255)
                                                                            cgAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCAACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGaa                                                                  <  4:1112028/151‑1 (MQ=255)
                                                                               aTCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATg                                                               <  2:785950/151‑1 (MQ=255)
                                                                                 cAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGaa                                                             <  4:1263887/151‑1 (MQ=255)
                                                                                 cAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGaa                                                             <  1:115481/151‑1 (MQ=255)
                                                                                 cAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGaa                                                             <  2:1623747/151‑1 (MQ=255)
                                                                                  aaaTTCCCCACCGCGGGTGTGTTAATCAGCAATTCGAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAAt                                                            >  2:467582/1‑151 (MQ=255)
                                                                                  aaaTTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAAt                                                            >  1:382626/1‑151 (MQ=255)
                                                                                  aaaTTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAAt                                                            >  2:351558/1‑151 (MQ=255)
                                                                                  aaaTTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAAt                                                            >  2:1516652/1‑151 (MQ=255)
                                                                                        cccACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATccacca                                                      <  1:191147/151‑1 (MQ=255)
                                                                                         ccACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGGCTTTTTCCTGCGCCGGAAATGAATCCACCAg                                                     <  3:474439/151‑1 (MQ=255)
                                                                                          cACCGCGGGTGGGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGt                                                    >  2:117785/1‑151 (MQ=255)
                                                                                           aCCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTc                                                   <  4:1401482/151‑1 (MQ=255)
                                                                                            ccGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCg                                                  >  4:1230381/1‑151 (MQ=255)
                                                                                               cgGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTc                                               <  2:118405/151‑1 (MQ=255)
                                                                                                     tgtTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGTAAATGAATCCACCAGTCGCTCAActg                                          <  2:1359491/150‑1 (MQ=255)
                                                                                                      gtTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAActgc                                         <  1:428548/150‑1 (MQ=255)
                                                                                                        tAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAActgcct                                       <  1:376154/150‑1 (MQ=255)
                                                                                                        tAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAActgcct                                       <  4:1569292/150‑1 (MQ=255)
                                                                                                         aaTCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAActgcctgc                                     <  3:395951/151‑1 (MQ=255)
                                                                                                           tCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTgcgc                                   >  3:37042/1‑151 (MQ=255)
                                                                                                           tCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTgcgc                                   >  4:582597/1‑151 (MQ=255)
                                                                                                            cAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTgcgc                                   <  4:1462370/150‑1 (MQ=255)
                                                                                                             aGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCgg                                 <  2:1217297/151‑1 (MQ=255)
                                                                                                              gCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGc                                >  4:173171/1‑151 (MQ=255)
                                                                                                                aaTTCAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGcac                              >  2:682400/1‑151 (MQ=255)
                                                                                                                aaTTCAAATAGCGCCACGCGTCCTTCCTGTTTATCGACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGcac                              >  2:1195879/1‑151 (MQ=255)
                                                                                                                    cAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGcaccac                           <  2:1130924/150‑1 (MQ=255)
                                                                                                                    cAAATAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGcaccac                           <  2:715581/150‑1 (MQ=255)
                                                                                                                     aaaTAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTTCCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCAcgc                         <  2:53501/151‑1 (MQ=255)
                                                                                                                       aTAGCGCCACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGt                       <  3:68697/151‑1 (MQ=255)
                                                                                                                              cACGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAAt                <  2:884825/151‑1 (MQ=255)
                                                                                                                               aCGCGTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATg               >  2:1553697/1‑151 (MQ=255)
                                                                                                                                 gcgTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGtt             <  2:1203171/151‑1 (MQ=255)
                                                                                                                                  cgTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTg            <  2:453855/151‑1 (MQ=255)
                                                                                                                                   gTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTg            <  1:189817/150‑1 (MQ=255)
                                                                                                                                   gTCCTTCCTGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGc           >  4:93289/1‑151 (MQ=255)
                                                                                                                                     ccttcctGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGcc          >  4:314165/1‑150 (MQ=255)
                                                                                                                                         cctGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGCCAGCa      <  2:1508264/150‑1 (MQ=255)
                                                                                                                                         cctGTTTATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGCCAGCAc     >  2:235643/1‑151 (MQ=255)
                                                                                                                                             tttATCTACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGCCAGCACCaa  >  4:1053391/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAATCCACCAGTCGCTCAACTGCCTGCGCGGCACCACGCGTATGTAATGTTGCCAGCACCAA  >  NC_000913/3094133‑3094423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: