Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,124,632 A→G S276S (AGT→AGC)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,124,6320AG100.0% 539.1 / NA 144S276S (AGT→AGC) glcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (85/59);  total (85/59)

Only 100 of 144 total aligned reads displayed.

AAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATT  >  NC_000913/3124485‑3124775
                                                                                                                                                   |                                                                                                                                               
aaGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctg                                                                                                                                              <  2:1251915/151‑1 (MQ=255)
 aGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctg                                                                                                                                              >  2:174355/1‑150 (MQ=255)
  gCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctgac                                                                                                                                            >  3:414121/1‑151 (MQ=255)
   cAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctgacc                                                                                                                                           <  2:327677/151‑1 (MQ=255)
     acacTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctgacctg                                                                                                                                         >  3:21326/1‑151 (MQ=255)
      cacTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctgacctg                                                                                                                                         >  4:1295445/1‑150 (MQ=255)
        cTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGctgacctg                                                                                                                                         <  2:850938/148‑1 (MQ=255)
        cTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACg                                                                                                                                      >  2:321760/1‑151 (MQ=255)
          ttttCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACg                                                                                                                                      <  1:353348/149‑1 (MQ=255)
           tttCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAGGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGc                                                                                                                                   >  2:30678/1‑151 (MQ=255)
             tCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCtt                                                                                                                                 >  3:47180/1‑151 (MQ=255)
              ccACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCttt                                                                                                                                <  3:184344/151‑1 (MQ=255)
              ccACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCtt                                                                                                                                 <  4:1072182/150‑1 (MQ=255)
               cACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCttt                                                                                                                                >  1:122226/1‑150 (MQ=255)
               cACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTa                                                                                                                               <  2:903011/151‑1 (MQ=255)
                aCTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTAt                                                                                                                              >  4:806994/1‑151 (MQ=255)
                  ttCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGACCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCg                                                                                                                            >  4:1621275/1‑151 (MQ=255)
                   tCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATc                                                                                                                             <  4:1154565/149‑1 (MQ=255)
                   tCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCgg                                                                                                                           >  4:862451/1‑151 (MQ=255)
                    cGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCgg                                                                                                                           <  4:1421577/150‑1 (MQ=255)
                    cGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGc                                                                                                                          >  2:1186438/1‑151 (MQ=255)
                    cGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGc                                                                                                                          >  2:990544/1‑151 (MQ=255)
                      ccGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCat                                                                                                                        >  3:126191/1‑151 (MQ=255)
                       cGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACTGCCAGTTCGCTGACCTGACGTGCTTTATCGGCata                                                                                                                       <  1:429864/151‑1 (MQ=255)
                        gTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCatat                                                                                                                      >  4:847604/1‑151 (MQ=255)
                          tCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCAtata                                                                                                                     <  4:827747/150‑1 (MQ=255)
                           cAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATaa                                                                                                                    <  2:1490998/150‑1 (MQ=255)
                           cAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAAc                                                                                                                   >  3:367915/1‑151 (MQ=255)
                           cAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAAc                                                                                                                   >  2:778913/1‑151 (MQ=255)
                             gCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGc                                                                                                                 >  3:67634/1‑151 (MQ=255)
                               ttttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCAt                                                                                                               >  2:1402614/1‑151 (MQ=255)
                                tttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATc                                                                                                              >  2:155291/1‑151 (MQ=255)
                                 ttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATc                                                                                                              >  4:1046534/1‑150 (MQ=255)
                                 ttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCg                                                                                                             >  2:792883/1‑151 (MQ=255)
                                 ttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCg                                                                                                             >  1:367634/1‑151 (MQ=255)
                                 ttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCg                                                                                                             >  1:438578/1‑151 (MQ=255)
                                  tGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGt                                                                                                            >  4:558278/1‑151 (MQ=255)
                                   gcgcATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGtt                                                                                                           >  1:238695/1‑151 (MQ=255)
                                      cATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGttttt                                                                                                        >  2:225749/1‑151 (MQ=255)
                                      cATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGttttt                                                                                                        >  4:1082502/1‑151 (MQ=255)
                                         gttgtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAg                                                                                                     <  3:129791/151‑1 (MQ=255)
                                            gtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCAt                                                                                                  <  4:800656/151‑1 (MQ=255)
                                               gggTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCt                                                                                                >  4:1537619/1‑150 (MQ=255)
                                               gggTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTg                                                                                               >  2:332436/1‑151 (MQ=255)
                                                   acacGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGccc                                                                                            >  2:1074985/1‑150 (MQ=255)
                                                     acGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCAt                                                                                          >  2:1176816/1‑150 (MQ=255)
                                                     acGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATa                                                                                         <  3:466397/151‑1 (MQ=255)
                                                       ggACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATAct                                                                                       >  4:1030100/1‑151 (MQ=255)
                                                         aCAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATActct                                                                                     <  1:94873/151‑1 (MQ=255)
                                                           aGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCttt                                                                                   <  4:402384/151‑1 (MQ=255)
                                                              ggAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGAc                                                                                >  4:1375891/1‑151 (MQ=255)
                                                              ggAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGAc                                                                                <  1:16865/151‑1 (MQ=255)
                                                                 aGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACaaa                                                                             <  2:625256/151‑1 (MQ=255)
                                                                 aGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACaa                                                                              >  2:633014/1‑150 (MQ=255)
                                                                 aGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACaa                                                                              >  2:1615304/1‑150 (MQ=255)
                                                                  ggCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAAc                                                                            <  3:136293/151‑1 (MQ=255)
                                                                      aGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAAcgcg                                                                         <  1:176635/150‑1 (MQ=255)
                                                                          ttttATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGcgccgc                                                                     >  2:1480321/1‑150 (MQ=255)
                                                                           tttATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGcgccgc                                                                     >  4:1668600/1‑149 (MQ=255)
                                                                            ttATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGc                                                                  >  1:338248/1‑151 (MQ=255)
                                                                             tATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGcc                                                                 >  4:1532800/1‑151 (MQ=255)
                                                                                cgccgcGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCt                                                              <  1:81085/151‑1 (MQ=255)
                                                                                 gccgcGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTg                                                             <  2:387654/151‑1 (MQ=255)
                                                                                  ccgcGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTgg                                                            >  4:1237844/1‑151 (MQ=255)
                                                                                   cgcgAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGc                                                           >  2:1340154/1‑151 (MQ=255)
                                                                                   cgcgAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGc                                                           >  4:533282/1‑151 (MQ=255)
                                                                                   cgcgAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGc                                                           <  4:30732/151‑1 (MQ=255)
                                                                                   cgcgAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGc                                                           >  4:1186314/1‑151 (MQ=255)
                                                                                   cgcgAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGc                                                           <  2:229120/151‑1 (MQ=255)
                                                                                    gcgAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCg                                                          >  1:238656/1‑151 (MQ=255)
                                                                                      gAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGt                                                        >  4:1617355/1‑151 (MQ=255)
                                                                                          tGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGc                                                    >  2:1613910/1‑151 (MQ=255)
                                                                                          tGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGc                                                    >  2:1179678/1‑151 (MQ=255)
                                                                                             cAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCaa                                                  >  2:1569221/1‑150 (MQ=255)
                                                                                                tttttCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAAt                                               <  3:453062/150‑1 (MQ=255)
                                                                                                    tCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGcc                                           <  3:168944/150‑1 (MQ=255)
                                                                                                     ccAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCt                                          <  4:886908/150‑1 (MQ=255)
                                                                                                          ggTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCAc                                    <  4:726911/151‑1 (MQ=255)
                                                                                                             tCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCt                                  <  4:226115/150‑1 (MQ=255)
                                                                                                              ccTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTg                                 <  3:312064/150‑1 (MQ=255)
                                                                                                                  gcgcAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTc                             <  3:138708/150‑1 (MQ=255)
                                                                                                                   cgcAGAAGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCa                            <  2:557506/150‑1 (MQ=255)
                                                                                                                      aGAATTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAAt                          <  2:1271470/149‑1 (MQ=255)
                                                                                                                         aGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCgg                     >  2:415856/1‑151 (MQ=255)
                                                                                                                         aGTTCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCgg                     >  3:591/1‑151 (MQ=255)
                                                                                                                            tCGACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGcc                  >  3:421037/1‑151 (MQ=255)
                                                                                                                              gACTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGccac                >  4:210429/1‑151 (MQ=255)
                                                                                                                               aCTAAATCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGccacc               >  2:195214/1‑151 (MQ=255)
                                                                                                                                  aaaTCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAg             >  2:110103/1‑150 (MQ=255)
                                                                                                                                   aaTCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGc           <  1:116774/151‑1 (MQ=255)
                                                                                                                                     tCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTTCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCAt         >  2:953755/1‑151 (MQ=255)
                                                                                                                                     tCGACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCAt         >  4:1397363/1‑151 (MQ=255)
                                                                                                                                       gACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATc        >  2:367393/1‑150 (MQ=255)
                                                                                                                                       gACCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATc        >  2:1541292/1‑150 (MQ=255)
                                                                                                                                        aCCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATc        <  4:721912/149‑1 (MQ=255)
                                                                                                                                        aCCGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCaa      <  2:253396/151‑1 (MQ=255)
                                                                                                                                         ccGCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCaa      <  4:331360/150‑1 (MQ=255)
                                                                                                                                           gCCAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAatat   >  2:99240/1‑151 (MQ=255)
                                                                                                                                             cAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatt  >  2:495908/1‑150 (MQ=255)
                                                                                                                                             cAGTTCGCTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAAtatt  >  1:106618/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGAAGTTCGACTAAATCGACCGCCAGTTCACTGACCTGACGTGCTTTATCGGCATATAACGCATCGTTTTTCAGCATCTGCCCATACTCTTTGACAAACGCGCCGCAGCCGCTGGCGGTTTGCAAAATTGCCTCGGCACCTGCTTCAATCGCGGGCCACCAGGCATCAATATT  >  NC_000913/3124485‑3124775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑