BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,085	T→C	V324A (GTG→GCG)	tolC →	transport channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,085	0	T	C	99.2%	453.2 / ‑4.3	126	V324A (GTG→GCG)	tolC	transport channel
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (63/62); total (63/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 126 total aligned reads displayed.

CTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCG  >  NC_000913/3178938‑3179231

cTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACGCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGa                                                                                                                                                  >  4:12891/1‑150 (MQ=255)

  aCCGGGATTTCTGACATCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGaaa                                                                                                                                                <  1:185614/150‑1 (MQ=255)

  aCCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGaaa                                                                                                                                                <  4:1513970/150‑1 (MQ=255)

   ccGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAAc                                                                                                                                               >  4:1050651/1‑150 (MQ=255)

     gggATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAgg                                                                                                                                            <  2:728450/151‑1 (MQ=255)

      ggATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGc                                                                                                                                           >  4:49790/1‑151 (MQ=255)

        aTTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGcac                                                                                                                                         >  3:126633/1‑151 (MQ=255)

           tCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGt                                                                                                                                      >  4:971076/1‑151 (MQ=255)

            cTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGt                                                                                                                                      >  4:1664726/1‑150 (MQ=255)

              gACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTAc                                                                                                                                    <  1:459021/150‑1 (MQ=255)

               acacCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACaa                                                                                                                                  <  4:1413297/151‑1 (MQ=255)

               acacCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACaa                                                                                                                                  <  2:1116054/151‑1 (MQ=255)

                 acCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACt                                                                                                                                >  2:1255796/1‑151 (MQ=255)

                       tataGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGt                                                                                                                            <  4:1013558/149‑1 (MQ=255)

                        ataGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCgg                                                                                                                         <  2:684640/151‑1 (MQ=255)

                          aGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTg                                                                                                                       <  4:942809/151‑1 (MQ=255)

                            cGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGcc                                                                                                                     <  2:258874/151‑1 (MQ=255)

                              gTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAg                                                                                                                   <  3:167169/151‑1 (MQ=255)

                              gTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAg                                                                                                                   <  4:28276/151‑1 (MQ=255)

                                 cGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCg                                                                                                                 <  2:263205/150‑1 (MQ=255)

                                    aaaCCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCa                                                                                                             <  3:334738/151‑1 (MQ=255)

                                      aCCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCaa                                                                                                            >  4:606377/1‑150 (MQ=255)

                                       cccGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAAca                                                                                                          <  1:259779/151‑2 (MQ=255)

                                         cGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTgg                                                                                                        <  4:289539/151‑1 (MQ=255)

                                         cGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTgg                                                                                                        <  2:618610/151‑1 (MQ=255)

                                          gtggtgCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTgg                                                                                                        >  2:1055985/1‑150 (MQ=255)

                                          gtggtgCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTgg                                                                                                        >  2:199262/1‑150 (MQ=255)

                                            ggtgCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGaaa                                                                                                     <  1:188938/151‑1 (MQ=255)

                                               gCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGt                                                                                                   <  4:169486/150‑1 (MQ=255)

                                                 cGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGcc                                                                                                >  4:410596/1‑151 (MQ=255)

                                                    tGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCAt                                                                                             >  2:154910/1‑151 (MQ=255)

                                                    tGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCAt                                                                                             >  1:213890/1‑151 (MQ=255)

                                                     ggTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCa                                                                                              <  4:730786/149‑1 (MQ=255)

                                                     ggTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATc                                                                                            <  4:1496905/151‑1 (MQ=255)

                                                      gTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCg                                                                                           >  4:419778/1‑151 (MQ=255)

                                                        aCCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGt                                                                                          <  2:1457942/150‑1 (MQ=255)

                                                            aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgt                                                                                     >  1:126957/1‑151 (MQ=255)

                                                            aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgt                                                                                     >  4:1640049/1‑151 (MQ=255)

                                                            aGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgt                                                                                     <  1:166450/151‑1 (MQ=255)

                                                             gTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgtc                                                                                    <  4:1030219/151‑1 (MQ=255)

                                                             gTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgtc                                                                                    >  2:1144544/1‑151 (MQ=255)

                                                              tATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgtcg                                                                                   >  1:331131/1‑151 (MQ=255)

                                                               aTGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGcgtcg                                                                                   >  4:466334/1‑150 (MQ=255)

                                                                tGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTg                                                                                 >  3:123986/1‑151 (MQ=255)

                                                                 gACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGc                                                                                <  1:47263/151‑1 (MQ=255)

                                                                   cGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGc                                                                                >  2:727056/1‑149 (MQ=255)

                                                                   cGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAg                                                                              <  3:413989/151‑1 (MQ=255)

                                                                       aGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGAcc                                                                           <  4:419251/150‑1 (MQ=255)

                                                                          aaTATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGc                                                                       >  1:147131/1‑151 (MQ=255)

                                                                           atatGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCg                                                                      >  3:3174/1‑151 (MQ=255)

                                                                            tatGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGt                                                                     >  1:367356/1‑151 (MQ=255)

                                                                             atGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGt                                                                     <  4:1357850/150‑1 (MQ=255)

                                                                               gggCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTtc                                                                   <  4:121612/150‑1 (MQ=255)

                                                                                   cAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTcctcct                                                              >  2:617473/1‑151 (MQ=255)

                                                                                     gAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTc                                                            <  2:1245535/151‑1 (MQ=255)

                                                                                         aaaGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaaca                                                        <  2:860663/151‑1 (MQ=255)

                                                                                           aGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCaacaac                                                      <  4:1015616/151‑1 (MQ=255)

                                                                                            gTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCaacaac                                                      >  4:1281795/1‑150 (MQ=255)

                                                                                                gCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTaa                                                 <  3:167166/151‑1 (MQ=255)

                                                                                                 ccTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAAt                                                <  1:412089/151‑1 (MQ=255)

                                                                                                 ccTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAAt                                                >  2:1109999/1‑151 (MQ=255)

                                                                                                   tGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGc                                              >  2:1658405/1‑151 (MQ=255)

                                                                                                    gAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATg                                               <  2:589810/149‑1 (MQ=255)

                                                                                                    gAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCa                                             <  1:210554/151‑1 (MQ=255)

                                                                                                      gCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCAt                                            <  2:49692/150‑1 (MQ=255)

                                                                                                         tctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCt                                          <  3:264722/149‑1 (MQ=255)

                                                                                                         tctcGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTa                                         <  4:1257379/150‑1 (MQ=255)

                                                                                                            cGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTAt                                        <  4:1297631/148‑1 (MQ=255)

                                                                                                            cGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATc                                       <  2:1299247/149‑1 (MQ=255)

                                                                                                            cGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCa                                      <  3:379326/150‑1 (MQ=255)

                                                                                                             gCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTcaaca                                                        >  1:94116/1‑131 (MQ=255)

                                                                                                             gCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGt                                    <  2:879051/151‑1 (MQ=255)

                                                                                                             gCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGt                                    >  3:238419/1‑151 (MQ=255)

                                                                                                              cTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGt                                    >  3:256690/1‑150 (MQ=255)

                                                                                                               tGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGt                                    >  4:639408/1‑149 (MQ=255)

                                                                                                               tGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGt                                    >  2:556435/1‑149 (MQ=255)

                                                                                                                 ccGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGc                                 >  2:909965/1‑150 (MQ=255)

                                                                                                                 ccGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGc                                 >  4:1621394/1‑150 (MQ=255)

                                                                                                                  cGATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCAt                               <  4:1246443/151‑1 (MQ=255)

                                                                                                                   gATTTATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCAtt                              <  1:316290/151‑1 (MQ=255)

                                                                                                                      ttATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAAc                           >  2:1389901/1‑151 (MQ=255)

                                                                                                                      ttATCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAAc                           >  2:544669/1‑151 (MQ=255)

                                                                                                                        aTCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACg                          <  1:300509/150‑1 (MQ=255)

                                                                                                                        aTCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGc                         >  2:250078/1‑151 (MQ=255)

                                                                                                                        aTCAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGCTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACg                          <  4:1251643/150‑1 (MQ=255)

                                                                                                                          cAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCt                       >  2:1550486/1‑151 (MQ=255)

                                                                                                                          cAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCt                       <  4:199592/151‑1 (MQ=255)

                                                                                                                          cAGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCt                       >  1:372822/1‑151 (MQ=255)

                                                                                                                           aGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCt                       >  2:1292042/1‑150 (MQ=255)

                                                                                                                           aGGGCGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTa                      <  1:353417/151‑1 (MQ=255)

                                                                                                                            gggCGGTATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTac                     >  2:120012/1‑151 (MQ=255)

                                                                                                                               cGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTacaaa                  <  2:845893/151‑1 (MQ=255)

                                                                                                                               cGGAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTacaaa                  <  3:213905/151‑1 (MQ=255)

                                                                                                                                ggAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTacaaac                 >  4:191392/1‑151 (MQ=255)

                                                                                                                                 gAATGGTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTacaaaca                <  2:664953/151‑1 (MQ=255)

                                                                                                                                     ggTTAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGcc            >  2:14123/1‑151 (MQ=255)

                                                                                                                                        tAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATc                                           <  1:203974/117‑1 (MQ=255)

                                                                                                                                        tAACTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGt          >  1:301994/1‑150 (MQ=255)

                                                                                                                                         aaCTCGCAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAAACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTa         <  4:493117/150‑1 (MQ=255)

                                                                                                                                               cAGGCGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCg  <  2:410010/151‑1 (MQ=255)

CTACCGGGATTTCTGACACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAATATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTTAACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGTGCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATCAGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCG  >  NC_000913/3178938‑3179231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`