Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,297,157 T→C T328A (ACA→GCA)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,297,1570TC100.0% 443.3 / NA 117T328A (ACA→GCA) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/65);  total (52/65)

Only 100 of 117 total aligned reads displayed.

ACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCT  >  NC_000913/3297009‑3297302
                                                                                                                                                    |                                                                                                                                                 
      gCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCAt                                                                                                                                            <  4:1124317/150‑1 (MQ=255)
       ccGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATaa                                                                                                                                          <  1:91963/151‑1 (MQ=255)
       ccGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATa                                                                                                                                           >  3:313919/1‑150 (MQ=255)
        cgcgTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAg                                                                                                                                         <  2:1035187/151‑1 (MQ=255)
        cgcgTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAg                                                                                                                                         <  4:585978/151‑1 (MQ=255)
          cgTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGc                                                                                                                                       >  4:1459873/1‑151 (MQ=255)
          cgTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGc                                                                                                                                       >  4:1215902/1‑151 (MQ=255)
          cgTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGc                                                                                                                                       >  1:449859/1‑151 (MQ=255)
           gTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGc                                                                                                                                       <  4:1314179/150‑1 (MQ=255)
            tCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCt                                                                                                                                      <  2:7721/150‑1 (MQ=255)
              ttATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTc                                                                                                                                   >  2:1446869/1‑151 (MQ=255)
               tATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCttt                                                                                                                                    <  2:620231/149‑1 (MQ=255)
                aTCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGc                                                                                                                                 >  4:1382336/1‑151 (MQ=255)
                          cAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCt                                                                                                                       >  4:682125/1‑151 (MQ=255)
                           aaCGATACAAACCGTAGGTCAGATAAGGCTTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAAc                                                                                                                          <  2:1661461/147‑1 (MQ=255)
                           aaCGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCtt                                                                                                                      >  2:766184/1‑151 (MQ=255)
                           aaCGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCtt                                                                                                                      <  2:870738/151‑1 (MQ=255)
                              gATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgc                                                                                                                   <  3:314336/151‑1 (MQ=255)
                                 aCAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCgca                                                                                                                <  4:525998/151‑1 (MQ=255)
                                      ccGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCAt                                                                                                            >  2:179585/1‑150 (MQ=255)
                                      ccGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATa                                                                                                           <  2:1608200/151‑1 (MQ=255)
                                       cGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATa                                                                                                           >  3:301550/1‑150 (MQ=255)
                                         tAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATc                                                                                                        >  1:332091/1‑151 (MQ=255)
                                            gTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGt                                                                                                     >  1:157334/1‑151 (MQ=255)
                                             tCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTg                                                                                                    >  1:274659/1‑151 (MQ=255)
                                               aGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGAc                                                                                                  <  4:809738/151‑1 (MQ=255)
                                               aGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGAc                                                                                                  <  2:369179/151‑1 (MQ=255)
                                                gATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACg                                                                                                 <  4:137720/151‑1 (MQ=255)
                                                gATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACg                                                                                                 >  4:1536599/1‑151 (MQ=255)
                                                 aTAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACgg                                                                                                <  3:38443/151‑1 (MQ=255)
                                                   aaGGCGTTTACGCCGCATCCGGCATGTTTTCCCTCAAATCAGAACAACAGCGCCAGTCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGc                                                                                               <  4:965513/150‑1 (MQ=255)
                                                    aGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCa                                                                                              >  3:116748/1‑150 (MQ=255)
                                                            aCGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTg                                                                                     <  2:39649/151‑1 (MQ=255)
                                                              gccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGcc                                                                                   >  4:544793/1‑151 (MQ=255)
                                                               ccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                  >  2:192094/1‑151 (MQ=255)
                                                               ccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                  <  2:1425608/151‑1 (MQ=255)
                                                               ccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                  <  4:452237/151‑1 (MQ=255)
                                                                 gcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCTCCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                  <  4:1032919/149‑1 (MQ=255)
                                                                  cATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCggg                                                                                >  2:79153/1‑150 (MQ=255)
                                                                   aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCggg                                                                                >  4:1646807/1‑149 (MQ=255)
                                                                    tCCGACATTTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAg                                                                              <  4:775914/150‑1 (MQ=255)
                                                                     ccGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGc                                                                             <  3:117041/150‑1 (MQ=255)
                                                                      cGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGt                                                                           >  2:1595898/1‑151 (MQ=255)
                                                                      cGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGt                                                                           <  4:1256833/151‑1 (MQ=255)
                                                                         cATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGtcat                                                                        >  1:38624/1‑151 (MQ=255)
                                                                          aTGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGtcatc                                                                       >  4:408766/1‑151 (MQ=255)
                                                                             ttttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATcaac                                                                    >  4:1490701/1‑151 (MQ=255)
                                                                             ttttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATcaa                                                                     <  1:169354/150‑1 (MQ=255)
                                                                                tCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAAt                                                                 >  2:514619/1‑151 (MQ=255)
                                                                                  ccTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGc                                                               >  4:1577222/1‑151 (MQ=255)
                                                                                  ccTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGc                                                               <  3:324683/151‑1 (MQ=255)
                                                                                  ccTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGc                                                               >  3:385691/1‑151 (MQ=255)
                                                                                   cTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGcc                                                              >  3:416180/1‑151 (MQ=255)
                                                                                     cAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGcc                                                              >  2:1684516/1‑149 (MQ=255)
                                                                                      aaaccggAACACCCGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCa                                                             <  2:498482/143‑1 (MQ=255)
                                                                                      aaaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgc                                                           <  2:774521/151‑1 (MQ=255)
                                                                                      aaaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgc                                                           <  1:92186/151‑1 (MQ=255)
                                                                                      aaaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAg                                                            <  1:313902/150‑1 (MQ=255)
                                                                                       aaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgcg                                                          >  4:116906/1‑151 (MQ=255)
                                                                                       aaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgc                                                           >  4:1171046/1‑150 (MQ=255)
                                                                                           aGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCg                                                       <  2:1513010/150‑1 (MQ=255)
                                                                                           aGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCg                                                       <  3:219174/150‑1 (MQ=255)
                                                                                            gAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGa                                                     <  3:32835/151‑1 (MQ=255)
                                                                                             aacaacAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGa                                                     <  3:235263/150‑1 (MQ=255)
                                                                                              acaacaGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc                                                   >  4:450922/1‑151 (MQ=255)
                                                                                              acaacaGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc                                                   <  3:313733/151‑1 (MQ=255)
                                                                                                aacaGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCgg                                                 >  4:763530/1‑151 (MQ=255)
                                                                                                     cgcCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTccc                                            >  2:1094909/1‑151 (MQ=255)
                                                                                                     cgcCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTcc                                             <  1:418087/150‑1 (MQ=255)
                                                                                                         aGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATAc                                        >  2:639873/1‑151 (MQ=255)
                                                                                                          gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATAcc                                       >  2:1404997/1‑151 (MQ=255)
                                                                                                            ccgccgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTg                                     <  1:464120/151‑1 (MQ=255)
                                                                                                               ccgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGcc                                   <  4:183128/150‑1 (MQ=255)
                                                                                                               ccgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCa                                  <  3:169846/151‑1 (MQ=255)
                                                                                                                cgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGcc                                   >  1:30367/1‑149 (MQ=255)
                                                                                                                 gACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGc                                >  4:305565/1‑151 (MQ=255)
                                                                                                                 gACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGc                                <  4:179885/151‑1 (MQ=255)
                                                                                                                  aCAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGca                               <  1:385674/151‑1 (MQ=255)
                                                                                                                   cAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcat                              >  2:1530014/1‑151 (MQ=255)
                                                                                                                    aaTCACCCCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                             <  2:1480101/151‑1 (MQ=255)
                                                                                                                    aaTCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                             <  4:1684822/151‑1 (MQ=255)
                                                                                                                    aaTCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                             <  4:721228/151‑1 (MQ=255)
                                                                                                                    aaTCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                             >  1:408859/1‑151 (MQ=255)
                                                                                                                       cacaCCAGACACTGCCCCCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatca                            <  2:1380613/149‑1 (MQ=255)
                                                                                                                       cacaCCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCatcatc                          <  2:1319283/151‑1 (MQ=255)
                                                                                                                       cacaCCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCatcatc                          <  4:63436/151‑1 (MQ=255)
                                                                                                                            cAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTg                     >  2:909203/1‑151 (MQ=255)
                                                                                                                             aGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGt                    <  3:284233/151‑1 (MQ=255)
                                                                                                                              gACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTa                   <  3:30369/151‑1 (MQ=255)
                                                                                                                              gACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTa                   <  3:38626/151‑1 (MQ=255)
                                                                                                                               acacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc                  >  2:830188/1‑151 (MQ=255)
                                                                                                                                cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc                  >  4:586203/1‑150 (MQ=255)
                                                                                                                                cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACa                 <  3:370137/151‑1 (MQ=255)
                                                                                                                                  cTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAAt               >  2:1406348/1‑151 (MQ=255)
                                                                                                                                    gCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCg             >  4:1416975/1‑151 (MQ=255)
                                                                                                                                     ccaccaTCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCgg            >  4:1575793/1‑151 (MQ=255)
                                                                                                                                             gCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctg    <  3:404380/151‑1 (MQ=255)
                                                                                                                                              ccccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgc   <  4:1042558/151‑1 (MQ=255)
                                                                                                                                              ccccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctg    <  2:1644806/150‑1 (MQ=255)
                                                                                                                                               cccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgct  <  4:79300/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACGCTTGCCGCGTCTTATCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCT  >  NC_000913/3297009‑3297302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑