Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,297,214 T→C S309G (AGT→GGT)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,297,2140TC100.0% 454.0 / NA 118S309G (AGT→GGT) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/64);  total (54/64)

Only 100 of 118 total aligned reads displayed.

TACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCT  >  NC_000913/3297068‑3297361
                                                                                                                                                  |                                                                                                                                                   
tACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCAc                                                                                                                                                  <  4:180291/150‑1 (MQ=255)
 aCGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTg                                                                                                                                                <  2:39649/151‑1 (MQ=255)
   gccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGcc                                                                                                                                              >  4:544793/1‑151 (MQ=255)
    ccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                                                                             <  4:452237/151‑1 (MQ=255)
    ccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                                                                             >  2:192094/1‑151 (MQ=255)
    ccgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                                                                             <  2:1425608/151‑1 (MQ=255)
      gcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCTCCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCg                                                                                                                                             <  4:1032919/149‑1 (MQ=255)
       cATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCggg                                                                                                                                           >  2:79153/1‑150 (MQ=255)
        aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCggg                                                                                                                                           >  4:1646807/1‑149 (MQ=255)
         tCCGACATTTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAg                                                                                                                                         <  4:775914/150‑1 (MQ=255)
          ccGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGc                                                                                                                                        <  3:117041/150‑1 (MQ=255)
           cGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGt                                                                                                                                      <  4:1256833/151‑1 (MQ=255)
           cGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGt                                                                                                                                      >  2:1595898/1‑151 (MQ=255)
               aTGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGtcatc                                                                                                                                  >  4:408766/1‑151 (MQ=255)
                  ttttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATcaac                                                                                                                               >  4:1490701/1‑151 (MQ=255)
                  ttttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATcaa                                                                                                                                <  1:169354/150‑1 (MQ=255)
                     tCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAAt                                                                                                                            >  2:514619/1‑151 (MQ=255)
                       ccTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGc                                                                                                                          <  3:324683/151‑1 (MQ=255)
                       ccTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGc                                                                                                                          >  3:385691/1‑151 (MQ=255)
                        cTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGcc                                                                                                                         >  3:416180/1‑151 (MQ=255)
                          cAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGcc                                                                                                                         >  2:1684516/1‑149 (MQ=255)
                           aaaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgc                                                                                                                      <  2:774521/151‑1 (MQ=255)
                           aaaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgc                                                                                                                      <  1:92186/151‑1 (MQ=255)
                           aaaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAg                                                                                                                       <  1:313902/150‑1 (MQ=255)
                            aaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgcg                                                                                                                     >  4:116906/1‑151 (MQ=255)
                            aaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgc                                                                                                                      >  4:1171046/1‑150 (MQ=255)
                             aTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAgcgc                                                                                                                    <  2:1402660/151‑1 (MQ=255)
                                aGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCgg                                                                                                                 >  4:1505976/1‑151 (MQ=255)
                                aGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCg                                                                                                                  <  2:1513010/150‑1 (MQ=255)
                                aGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCg                                                                                                                  <  3:219174/150‑1 (MQ=255)
                                  aacaacAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGa                                                                                                                <  3:235263/150‑1 (MQ=255)
                                   acaacaGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc                                                                                                              >  4:450922/1‑151 (MQ=255)
                                   acaacaGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc                                                                                                              <  3:313733/151‑1 (MQ=255)
                                     aacaGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCgg                                                                                                            >  4:763530/1‑151 (MQ=255)
                                          cgcCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTccc                                                                                                       >  2:1094909/1‑151 (MQ=255)
                                          cgcCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTcc                                                                                                        <  4:540476/150‑1 (MQ=255)
                                          cgcCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTcc                                                                                                        <  1:418087/150‑1 (MQ=255)
                                              aGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATAc                                                                                                   >  2:639873/1‑151 (MQ=255)
                                                 ccgccgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTg                                                                                                <  1:464120/151‑1 (MQ=255)
                                                    ccgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCa                                                                                             <  3:169846/151‑1 (MQ=255)
                                                     cgACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGcc                                                                                              >  1:30367/1‑149 (MQ=255)
                                                      gACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGc                                                                                           >  3:379752/1‑151 (MQ=255)
                                                      gACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGc                                                                                           <  4:179885/151‑1 (MQ=255)
                                                      gACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGc                                                                                           >  4:305565/1‑151 (MQ=255)
                                                       aCAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGca                                                                                          <  1:385674/151‑1 (MQ=255)
                                                         aaTCACCCCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                                                                                        <  2:1480101/151‑1 (MQ=255)
                                                         aaTCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                                                                                        >  1:408859/1‑151 (MQ=255)
                                                         aaTCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc                                                                                        <  4:1684822/151‑1 (MQ=255)
                                                            cacaCCAGACACTGCCCCCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatca                                                                                       <  2:1380613/149‑1 (MQ=255)
                                                            cacaCCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCatcatc                                                                                     <  2:1319283/151‑1 (MQ=255)
                                                            cacaCCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCatcatc                                                                                     <  4:63436/151‑1 (MQ=255)
                                                                 cAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTg                                                                                >  2:909203/1‑151 (MQ=255)
                                                                  aGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGt                                                                               <  3:284233/151‑1 (MQ=255)
                                                                   gACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTa                                                                              <  3:38626/151‑1 (MQ=255)
                                                                   gACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTa                                                                              <  3:30369/151‑1 (MQ=255)
                                                                    acacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc                                                                             >  2:830188/1‑151 (MQ=255)
                                                                     cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc                                                                             >  4:586203/1‑150 (MQ=255)
                                                                     cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACa                                                                            <  3:370137/151‑1 (MQ=255)
                                                                       cTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAAt                                                                          >  2:1406348/1‑151 (MQ=255)
                                                                         gCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCg                                                                        >  4:1416975/1‑151 (MQ=255)
                                                                          ccaccaTCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCgg                                                                       >  4:1575793/1‑151 (MQ=255)
                                                                                tCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTc                                                                 >  4:750379/1‑151 (MQ=255)
                                                                                   ccccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgc                                                              <  4:1042558/151‑1 (MQ=255)
                                                                                   ccccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctg                                                               <  2:1644806/150‑1 (MQ=255)
                                                                                    cccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgct                                                             <  4:79300/151‑1 (MQ=255)
                                                                                       tgccAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGcc                                                          >  3:265472/4‑151 (MQ=255)
                                                                                          cAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTg                                                       <  4:1278175/151‑1 (MQ=255)
                                                                                           aGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTgg                                                      <  3:356178/151‑1 (MQ=255)
                                                                                               aTAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAAt                                                  <  2:1488835/151‑1 (MQ=255)
                                                                                                 aaGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATg                                                 <  2:321575/150‑1 (MQ=255)
                                                                                                    gCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGacaa                                             <  1:176265/151‑1 (MQ=255)
                                                                                                     cTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGacaa                                             >  3:84239/1‑150 (MQ=255)
                                                                                                          gCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGc                                       <  3:18436/151‑1 (MQ=255)
                                                                                                             gggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCAt                                     <  4:1597863/150‑1 (MQ=255)
                                                                                                              ggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATcc                                   >  2:1572738/1‑151 (MQ=255)
                                                                                                              ggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATcc                                   <  3:33918/151‑1 (MQ=255)
                                                                                                                aaCGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCt                                  <  2:1656556/150‑1 (MQ=255)
                                                                                                                 aCGCTTTGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGc                                >  4:533781/1‑151 (MQ=255)
                                                                                                                     tttGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTAcc                            >  4:1600972/1‑151 (MQ=255)
                                                                                                                     tttGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTAcc                            >  2:901623/1‑151 (MQ=255)
                                                                                                                      ttGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTAcc                            <  2:760077/150‑1 (MQ=255)
                                                                                                                      ttGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTAcc                            <  2:418938/150‑1 (MQ=255)
                                                                                                                      ttGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTAcc                            <  3:408876/150‑1 (MQ=255)
                                                                                                                       tGCGCAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCg                           <  4:1530523/150‑1 (MQ=255)
                                                                                                                        gcgcAGCATAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGcc                         >  2:1216222/1‑151 (MQ=255)
                                                                                                                           cagcaTAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATc                      >  4:1174357/1‑151 (MQ=255)
                                                                                                                             gcaTAATCAGTGACGGCAACCCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCAt                       <  2:681188/148‑1 (MQ=255)
                                                                                                                                tAATCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAGCAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGccac                 >  4:820571/1‑151 (MQ=255)
                                                                                                                                 aaTCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGccacc                >  2:110908/1‑151 (MQ=255)
                                                                                                                                  aTCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGccacca               <  2:247172/151‑1 (MQ=255)
                                                                                                                                  aTCAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGccacc                >  2:660294/1‑150 (MQ=255)
                                                                                                                                    cAGTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCaccacc             <  2:1458050/151‑1 (MQ=255)
                                                                                                                                      gTGACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCAccaccca           <  2:585394/151‑1 (MQ=255)
                                                                                                                                        gACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAACCAAACGTCCTGCTATCGCCATCGCCAacaccca           >  4:186987/1‑149 (MQ=255)
                                                                                                                                        gACGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCAccacccac          >  2:339671/1‑150 (MQ=255)
                                                                                                                                         aCGGCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACAt        >  3:46748/1‑151 (MQ=255)
                                                                                                                                           ggCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCa      <  3:321199/151‑1 (MQ=255)
                                                                                                                                            gCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATc       >  1:118561/1‑149 (MQ=255)
                                                                                                                                            gCAAACCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAg     <  2:1214372/151‑1 (MQ=255)
                                                                                                                                               aaCCCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCt  <  4:661195/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTGCTACCGCCATCGCCACCACCCACATCAGGCT  >  NC_000913/3297068‑3297361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑