Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 322,322 C→T R239C (CGC→TGC)  ykgE → cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913322,3220CT99.0% 385.6 / ‑5.2 99R239C (CGC→TGC) ykgEcysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (57/41);  total (57/42)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGA  >  NC_000913/322174‑322468
                                                                                                                                                    |                                                                                                                                                  
ggTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGc                                                                                                                                                  >  3:428394/1‑151 (MQ=255)
ggTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGc                                                                                                                                                  >  4:345403/1‑151 (MQ=255)
     aagaaaAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAgg                                                                                                                                             >  2:1634066/1‑151 (MQ=255)
      agaaaAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAgg                                                                                                                                             >  4:1317177/1‑150 (MQ=255)
      agaaaAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGa                                                                                                                                            <  4:1652833/151‑1 (MQ=255)
          aaGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGAtata                                                                                                                                        <  2:1303600/151‑1 (MQ=255)
           aGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATaa                                                                                                                                       <  4:1627671/151‑1 (MQ=255)
                gcgcACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATg                                                                                                                                  <  2:578276/151‑1 (MQ=255)
                gcgcACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATg                                                                                                                                  <  2:281495/151‑1 (MQ=255)
                 cgcACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGt                                                                                                                                 >  2:226289/1‑151 (MQ=255)
                  gcACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTc                                                                                                                                >  2:1667419/1‑151 (MQ=255)
                   cACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCg                                                                                                                               >  4:1121268/1‑151 (MQ=255)
                    aCCTGATGGAAGTCCGCGCTGAGTATTTAATTGGTGCTGACGTGCGTTGCCTGCTAAACATCAGTGGGTGATTTTATCGATAAGAGCAGAAACCCCAAGTGCTGCATATTGCTCAAATGTTTTTCCCCCGCTGAggcttttacgattc                                                                                                                                 <  2:532200/148‑13 (MQ=255)
                     ccTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGAt                                                                                                                             >  4:1156348/1‑151 (MQ=255)
                     ccTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGAt                                                                                                                             >  2:105768/1‑151 (MQ=255)
                        gATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATc                                                                                                                            <  2:726654/149‑1 (MQ=255)
                         aTGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCaa                                                                                                                          >  2:1635633/1‑150 (MQ=255)
                         aTGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCaa                                                                                                                          >  3:294486/1‑150 (MQ=255)
                            gAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAAcc                                                                                                                      >  4:362504/1‑151 (MQ=255)
                             aaGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCa                                                                                                                     <  4:135601/151‑1 (MQ=255)
                                tCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGt                                                                                                                   >  2:195148/1‑150 (MQ=255)
                                 ccGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTaa                                                                                                                 <  2:1459410/151‑1 (MQ=255)
                                    cccTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATAc                                                                                                              <  4:853799/151‑1 (MQ=255)
                                     ccTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATAc                                                                                                              >  2:1382759/1‑150 (MQ=255)
                                     ccTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACa                                                                                                             <  3:170471/151‑1 (MQ=255)
                                        gAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATAc                                                                                                              >  4:1510953/1‑147 (MQ=255)
                                        gAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAg                                                                                                            <  4:816147/149‑1 (MQ=255)
                                            aTTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGAtttt                                                                                                       >  2:647958/1‑150 (MQ=255)
                                                aaTTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGac                                                                                                  >  2:147706/1‑151 (MQ=255)
                                                aaTTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGac                                                                                                  >  2:1628762/1‑151 (MQ=255)
                                                aaTTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGac                                                                                                  <  1:442946/151‑1 (MQ=255)
                                                 aTTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGaca                                                                                                 <  4:664405/151‑1 (MQ=255)
                                                  ttGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGacac                                                                                                >  4:364365/1‑151 (MQ=255)
                                                   tGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGacac                                                                                                <  2:365681/150‑1 (MQ=255)
                                                    ggTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGc                                                                                              >  2:596647/1‑151 (MQ=255)
                                                      tGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCAt                                                                                            >  2:477220/1‑151 (MQ=255)
                                                       gCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATc                                                                                           >  3:438920/1‑151 (MQ=255)
                                                         tGACGTGAGTTGCCTGCTAAACATCACTGGGCGATTACAACGGGAAGGGCAGCAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCTTCCg                                                                                         >  1:244107/1‑151 (MQ=255)
                                                          gACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATcc                                                                                          <  1:457753/149‑1 (MQ=255)
                                                           aCGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTc                                                                                       <  2:795588/151‑1 (MQ=255)
                                                            cGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCa                                                                                      <  2:681724/151‑1 (MQ=255)
                                                             gTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTc                                                                                       >  4:1297850/1‑149 (MQ=255)
                                                             gTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTc                                                                                       >  2:162114/1‑149 (MQ=255)
                                                             gTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTc                                                                                       >  4:1655319/1‑149 (MQ=255)
                                                               gAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGc                                                                                    <  4:227324/150‑1 (MQ=255)
                                                                    gCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTg                                                                              >  1:260298/1‑151 (MQ=255)
                                                                    gCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTg                                                                              >  2:802101/1‑151 (MQ=255)
                                                                    gCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTg                                                                              >  2:148354/1‑151 (MQ=255)
                                                                     ccTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTg                                                                              >  2:344659/1‑150 (MQ=255)
                                                                     ccTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGa                                                                             <  2:1484659/151‑1 (MQ=255)
                                                                          tAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGa                                                                             >  2:1336158/1‑146 (MQ=255)
                                                                           aaaCATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATca                                                                       <  2:899717/151‑2 (MQ=255)
                                                                            aaCATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCg                                                                      >  4:1488/1‑151 (MQ=255)
                                                                             aCATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGa                                                                     <  3:72587/151‑1 (MQ=255)
                                                                               aTCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATc                                                                   >  3:295893/1‑151 (MQ=255)
                                                                                 cAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCAt                                                                 <  4:1671489/151‑1 (MQ=255)
                                                                                  aGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATg                                                                <  4:1089338/151‑1 (MQ=255)
                                                                                  aGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATg                                                                >  4:185920/1‑151 (MQ=255)
                                                                                     gggCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATgcgc                                                             >  2:172232/1‑151 (MQ=255)
                                                                                     gggCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATgcgc                                                             >  4:403703/1‑151 (MQ=255)
                                                                                       gCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCa                                                            >  4:1473075/1‑150 (MQ=255)
                                                                                         gATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCaa                                                           >  3:288067/1‑149 (MQ=255)
                                                                                          aTTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGc                                                        >  2:109438/1‑151 (MQ=255)
                                                                                          aTTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGc                                                        >  4:729795/1‑151 (MQ=255)
                                                                                           ttACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCg                                                       >  2:1236049/1‑151 (MQ=255)
                                                                                            tACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCgg                                                      >  4:1120337/1‑151 (MQ=255)
                                                                                            tACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCgg                                                      >  4:1301593/1‑151 (MQ=255)
                                                                                            tACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCgg                                                      >  4:1606073/1‑151 (MQ=255)
                                                                                              cAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTg                                                    <  3:294394/151‑1 (MQ=255)
                                                                                               aaCGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTgg                                                   >  2:756494/1‑151 (MQ=255)
                                                                                                 cGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCa                                                 <  4:417849/151‑1 (MQ=255)
                                                                                                  gggAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCaa                                                <  1:100191/151‑1 (MQ=255)
                                                                                                       gggCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAAcgcg                                           >  4:545415/1‑151 (MQ=255)
                                                                                                       gggCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAAcgcg                                           <  2:1412913/151‑1 (MQ=255)
                                                                                                         gCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACgcgc                                          >  2:180632/1‑150 (MQ=255)
                                                                                                           aGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCgcagc                                       <  4:105946/151‑1 (MQ=255)
                                                                                                            gAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCgcagca                                      <  3:275491/151‑1 (MQ=255)
                                                                                                             aaaGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGcagcag                                     <  2:1315531/151‑1 (MQ=255)
                                                                                                               aGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCg                                   <  1:260727/151‑1 (MQ=255)
                                                                                                               aGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCg                                   <  4:40760/151‑1 (MQ=255)
                                                                                                                gTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGt                                  >  4:567846/1‑151 (MQ=255)
                                                                                                                  cAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTa                                 <  4:1642433/150‑1 (MQ=255)
                                                                                                                  cAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTAt                                >  4:670454/1‑151 (MQ=255)
                                                                                                                  cAAAGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGAAt                                >  1:22166/1‑151 (MQ=255)
                                                                                                                     aGTGATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTgg                             >  2:83669/1‑151 (MQ=255)
                                                                                                                        gATGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTgggg                           <  4:1005286/150‑1 (MQ=255)
                                                                                                                         aTGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGc                          >  3:345547/1‑150 (MQ=255)
                                                                                                                         aTGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGc                          >  2:858233/1‑150 (MQ=255)
                                                                                                                          tGCATATTGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCa                         >  4:596656/1‑150 (MQ=255)
                                                                                                                                 tGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGc                  >  2:1050806/1‑150 (MQ=255)
                                                                                                                                 tGCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGc                  <  2:1168712/150‑1 (MQ=255)
                                                                                                                                  gCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCa                 >  2:1107233/1‑150 (MQ=255)
                                                                                                                                  gCTGAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCa                 <  1:117455/150‑1 (MQ=255)
                                                                                                                                     gAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCaaaa              >  3:222349/1‑150 (MQ=255)
                                                                                                                                     gAAGTGTTGATGAGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCaaa               <  3:309776/149‑1 (MQ=255)
                                                                                                                                             gatgaGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCg      <  2:895264/150‑1 (MQ=255)
                                                                                                                                             gatgaGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCg      <  4:1637625/150‑1 (MQ=255)
                                                                                                                                             gatgaGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGa     <  2:1253105/151‑1 (MQ=255)
                                                                                                                                                gaGCTGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGa  <  4:1384489/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGTGAAAGAAAAGGTTGCGCACCTGATGGAAGTCCGCCCTGAGTATTTAATTGGTGCTGACGTGAGTTGCCTGCTAAACATCAGTGGGCGATTACAACGGGAAGGGCAGAAAGTCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGA  >  NC_000913/322174‑322468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: