Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,332,634 T→C E34E (GAA→GAG yhbE ← EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,332,6340TC100.0% 365.0 / NA 100E34E (GAA→GAGyhbEEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/45);  total (55/45)

CAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATG  >  NC_000913/3332486‑3332781
                                                                                                                                                    |                                                                                                                                                   
cAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTc                                                                                                                                                   >  2:63214/1‑151 (MQ=255)
    cAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATc                                                                                                                                               <  4:58260/151‑1 (MQ=255)
      gCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCAc                                                                                                                                             <  4:122875/151‑1 (MQ=255)
       cccGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCAcc                                                                                                                                            <  4:1205550/151‑1 (MQ=255)
        ccGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGGAACGGTAAAACACGATTGTCGGAGGCTCCATCAcc                                                                                                                                            >  2:42444/1‑150 (MQ=255)
           gcggTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTcc                                                                                                                                         >  3:430879/1‑150 (MQ=255)
            cggTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTcc                                                                                                                                         >  4:1590117/1‑149 (MQ=255)
                 gCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCt                                                                                                                                  <  2:643971/151‑1 (MQ=255)
                  ccACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTg                                                                                                                                 <  4:1127355/151‑1 (MQ=255)
                   cACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCCCCGCAAGAATAGCACCCAGGCCAATACTCGCCATCAAGGAACGGTAAAACACGATTGTCGGGGGCTCCATCCCCTCCAGCACCTg                                                                                                                                 >  2:1122119/1‑150 (MQ=255)
                   cACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGc                                                                                                                                >  2:1556923/1‑151 (MQ=255)
                      tGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCtt                                                                                                                              >  2:477696/1‑150 (MQ=255)
                       gCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCtt                                                                                                                              <  2:233132/149‑1 (MQ=255)
                         caacaaAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCAtt                                                                                                                          >  2:168800/1‑151 (MQ=255)
                          aacaaAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTg                                                                                                                         <  4:686849/151‑1 (MQ=255)
                           acaaAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGc                                                                                                                        <  2:221461/151‑1 (MQ=255)
                            caaAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCg                                                                                                                       >  1:444620/1‑151 (MQ=255)
                              aaaTCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGAt                                                                                                                     <  4:135022/151‑1 (MQ=255)
                                 tCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTgg                                                                                                                  >  4:829529/1‑151 (MQ=255)
                                 tCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTgg                                                                                                                  >  4:1248331/1‑151 (MQ=255)
                                  cAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTgg                                                                                                                  <  4:695038/150‑1 (MQ=255)
                                   aaCCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCa                                                                                                                <  2:1581528/151‑1 (MQ=255)
                                    aCCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCaa                                                                                                               <  2:609189/151‑1 (MQ=255)
                                     ccAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAAc                                                                                                              <  4:300775/151‑1 (MQ=255)
                                         cGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGccc                                                                                                          >  2:1578694/1‑151 (MQ=255)
                                            ggCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGccccc                                                                                                        <  1:356532/150‑1 (MQ=255)
                                             gCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGcccccc                                                                                                       >  2:786808/1‑150 (MQ=255)
                                             gCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCa                                                                                                      <  2:987030/151‑1 (MQ=255)
                                               ttACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGc                                                                                                    >  4:642761/1‑151 (MQ=255)
                                                tACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAg                                                                                                     <  2:849982/149‑1 (MQ=255)
                                                tACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAg                                                                                                     <  4:1677336/149‑1 (MQ=255)
                                                tACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAAAGCAACCCAGGCCAAAACTCGCCATCAAGAAACGGTAAAACACGATTGTCCGAGGCGCCATAACCTCCAGCACCTGCTTTAATTCGATTGGGAACGcccccc                                                                                                       >  2:675685/1‑147 (MQ=255)
                                                 aCGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCa                                                                                                   >  2:1495788/1‑150 (MQ=255)
                                                   gAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAAt                                                                                                <  2:603691/151‑1 (MQ=255)
                                                   gAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAAt                                                                                                >  2:517437/1‑151 (MQ=255)
                                                    aaaCACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAAtt                                                                                               <  4:171290/151‑1 (MQ=255)
                                                    aaaCACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAAtt                                                                                               <  4:175010/151‑1 (MQ=255)
                                                       cacGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGc                                                                                             <  2:57383/150‑1 (MQ=255)
                                                        acGCGTAATGGCGGCAACCTCCTCTTCACCGCAAGAATGGCACCCAGGCCAAGACTCGCCATCAAGAAACGGAAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCAGCATCTTCATTGCGATTGGCAACGCCCCCCAGCACATTGc                                                                                             >  2:688285/1‑149 (MQ=255)
                                                           cttccgattggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTgtgg                                                                                        >  2:165895/10‑151 (MQ=255)
                                                              aaTGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTgtggtg                                                                                      >  4:725030/1‑150 (MQ=255)
                                                              aaTGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGa                                                                                     <  2:849695/151‑1 (MQ=255)
                                                               aTGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTgtggtg                                                                                      >  3:88049/1‑149 (MQ=255)
                                                                tGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAgc                                                                                   >  4:178497/1‑151 (MQ=255)
                                                                 ggcggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAgc                                                                                   <  4:1384574/150‑1 (MQ=255)
                                                                  gcggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAgcgc                                                                                 <  3:357945/151‑1 (MQ=255)
                                                                   cggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGcc                                                                                >  4:1271513/1‑151 (MQ=255)
                                                                    ggcAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCa                                                                               <  4:696773/151‑1 (MQ=255)
                                                                        aCCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCaaa                                                                             >  2:802022/1‑149 (MQ=255)
                                                                           tcttctTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaat                                                                        >  2:172970/1‑151 (MQ=255)
                                                                           tcttctTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaa                                                                         >  2:662562/1‑150 (MQ=255)
                                                                            cttcttCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaa                                                                         <  1:372001/149‑1 (MQ=255)
                                                                              tcttcACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaat                                                                        <  4:787842/148‑1 (MQ=255)
                                                                                  cACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaatgccaatg                                                                 <  3:83894/151‑1 (MQ=255)
                                                                                  cACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaatgccaatg                                                                 >  2:1232780/1‑151 (MQ=255)
                                                                                   aCCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGaatgccaa                                                                   >  4:1437877/1‑148 (MQ=255)
                                                                                      gCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctg                                                             >  4:513601/1‑151 (MQ=255)
                                                                                      gCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctg                                                             <  2:1412746/151‑1 (MQ=255)
                                                                                      gCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctg                                                             >  1:14858/1‑151 (MQ=255)
                                                                                       cAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctgc                                                            <  4:661928/151‑1 (MQ=255)
                                                                                        aaGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAAtgcctgcc                                                           >  4:670457/1‑151 (MQ=255)
                                                                                         aGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATgcctgcct                                                          >  4:602820/1‑151 (MQ=255)
                                                                                          gAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGcctgcctg                                                         <  1:355286/151‑1 (MQ=255)
                                                                                           aaTGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCctgcctgc                                                        >  4:967398/1‑151 (MQ=255)
                                                                                               gCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                    <  4:757823/151‑1 (MQ=255)
                                                                                               gCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTgctgct                                                    >  1:449589/1‑151 (MQ=255)
                                                                                                 aCCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTc                                                  >  2:1509260/1‑151 (MQ=255)
                                                                                                     aGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATAc                                              <  4:1307719/151‑1 (MQ=255)
                                                                                                       gCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCg                                            <  3:213328/151‑1 (MQ=255)
                                                                                                        ccAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGt                                           <  4:597275/151‑1 (MQ=255)
                                                                                                         cAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGt                                           >  2:1211203/1‑150 (MQ=255)
                                                                                                            tACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTc                                       >  4:1364396/1‑151 (MQ=255)
                                                                                                             aCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTcc                                      >  1:294382/1‑151 (MQ=255)
                                                                                                               tCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCt                                    >  2:534613/1‑151 (MQ=255)
                                                                                                                cGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCt                                    <  3:18558/150‑1 (MQ=255)
                                                                                                                 gCCATCAAGAAACGGTAAAACGCGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGc                                  >  2:713267/1‑151 (MQ=255)
                                                                                                                  ccATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTccc                                     >  4:348057/1‑147 (MQ=255)
                                                                                                                  ccATCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCa                                 <  2:966144/151‑1 (MQ=255)
                                                                                                                    aTCAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCaa                                >  2:1655760/1‑150 (MQ=255)
                                                                                                                      cAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgc                             >  3:201209/1‑151 (MQ=255)
                                                                                                                      cAAGAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgc                             >  1:136833/1‑151 (MQ=255)
                                                                                                                         gAAACGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAgcgc                           >  2:297403/1‑150 (MQ=255)
                                                                                                                          aaaCGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCa                          <  1:39607/150‑1 (MQ=255)
                                                                                                                          aaaCGGTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCAt                         >  2:1240215/1‑151 (MQ=255)
                                                                                                                              ggTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGc                     >  2:222730/1‑151 (MQ=255)
                                                                                                                               gTAAAACACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg                    >  2:1098800/1‑151 (MQ=255)
                                                                                                                                 aaaaCACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCgg                   <  2:6483/150‑1 (MQ=255)
                                                                                                                                  aaaCACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                 >  4:134786/1‑151 (MQ=255)
                                                                                                                                   aaCACGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAt                 <  3:185415/150‑1 (MQ=255)
                                                                                                                                       cGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACg            <  2:724054/151‑1 (MQ=255)
                                                                                                                                       cGATTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACg            >  1:292801/1‑151 (MQ=255)
                                                                                                                                         aTTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc          >  3:38999/1‑151 (MQ=255)
                                                                                                                                         aTTGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc          <  4:859924/151‑1 (MQ=255)
                                                                                                                                           tGTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGc        >  4:773508/1‑151 (MQ=255)
                                                                                                                                            gTCGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGc        >  2:1609903/1‑150 (MQ=255)
                                                                                                                                              cGGAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAc      <  4:173254/150‑1 (MQ=255)
                                                                                                                                               ggAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc     >  3:147792/1‑150 (MQ=255)
                                                                                                                                                gAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt   <  2:178198/151‑1 (MQ=255)
                                                                                                                                                gAGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAt   <  3:213418/151‑1 (MQ=255)
                                                                                                                                                 aGGCTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATg  >  4:514948/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGATTGGCAACGCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATG  >  NC_000913/3332486‑3332781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: